Effect of ethanol-conditioned dentine on sealer penetration into dentinal tubules
Aim: The aim of this study was to evaluate the effect of ethanol-conditioned dentin on endodontic sealer penetration into dentinal tubules by confocal laser scanning microscopy (CLSM). Methods: Forty human maxillary anterior teeth were instrumented and divided into four groups (n = 10) according to...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Campinas
2021-05-01
|
| Series: | Brazilian Journal of Oral Sciences |
| Subjects: | |
| Online Access: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8661194 |
| _version_ | 1818823371731763200 |
|---|---|
| author | Carlos de Morais Souto Pantoja Diogo Henrique da Silva Adriana de Jesus Soares Caio Cezar Randi Ferraz Brenda Paula Figueiredo de Almeida Gomes Alexandre Augusto Zaia José Flávio Affonso de Almeida |
| author_facet | Carlos de Morais Souto Pantoja Diogo Henrique da Silva Adriana de Jesus Soares Caio Cezar Randi Ferraz Brenda Paula Figueiredo de Almeida Gomes Alexandre Augusto Zaia José Flávio Affonso de Almeida |
| author_sort | Carlos de Morais Souto Pantoja |
| collection | DOAJ |
| description | Aim: The aim of this study was to evaluate the effect of ethanol-conditioned dentin on endodontic sealer penetration into dentinal tubules by confocal laser scanning microscopy (CLSM). Methods: Forty human maxillary anterior teeth were instrumented and divided into four groups (n = 10) according to the drying methods: 1) wet: vacuum only, 2) paper points: vacuum + absorbent paper points, (3) 70% ethanol: 70% ethanol (1 min) + vacuum + absorbent paper points, and (4) 100% ethanol: 100% ethanol (1 min) + vacuum + absorbent paper points. All root canals were filled with resin-based endodontic sealer. Four sections from each third (cervical, middle, and apical) were examined by CLSM. Root canal wall perimeter infiltrated by sealer, maximum depth of sealer penetration, percentage of penetrated area, and fluorescence intensity of rhodamine B were measured. Statistical analysis was performed by analysis of variance and Tukey’s tests (α = 0.05). Results: No statistical difference was found when percentage of root canal wall coverage infiltrated by sealer were compared. The groups in which ethanol solutions were used presented greater depth of sealer penetration, higher percentage of penetrated area, and higher fluorescence intensity of rhodamine B (p< 0.05) when compared with the wet and paper point groups. Overall, 100% ethanol produced better results than 70% ethanol, except for rhodamine B intensity (cervical third). In addition, the absorbent paper points drying method behaved better than did vacuum only group, except for rhodamine B intensity (apical third). Conclusion: Ethanol-conditioned dentin improved the penetration of resin-based sealer into dentinal tubules, especially at the concentration of 100%. |
| first_indexed | 2024-12-18T23:38:55Z |
| format | Article |
| id | doaj.art-bd4119ff495a46dabf53ac650f053038 |
| institution | Directory Open Access Journal |
| issn | 1677-3225 |
| language | English |
| last_indexed | 2024-12-18T23:38:55Z |
| publishDate | 2021-05-01 |
| publisher | Universidade Estadual de Campinas |
| record_format | Article |
| series | Brazilian Journal of Oral Sciences |
| spelling | doaj.art-bd4119ff495a46dabf53ac650f0530382022-12-21T20:47:28ZengUniversidade Estadual de CampinasBrazilian Journal of Oral Sciences1677-32252021-05-01200010.20396/bjos.v20i00.8661194Effect of ethanol-conditioned dentine on sealer penetration into dentinal tubulesCarlos de Morais Souto Pantoja0Diogo Henrique da Silva1Adriana de Jesus Soares2Caio Cezar Randi Ferraz3Brenda Paula Figueiredo de Almeida Gomes4Alexandre Augusto Zaia5José Flávio Affonso de Almeida6University of CampinasUniversity of CampinasUniversity of CampinasUniversity of CampinasUniversity of CampinasUniversity of CampinasUniversity of CampinasAim: The aim of this study was to evaluate the effect of ethanol-conditioned dentin on endodontic sealer penetration into dentinal tubules by confocal laser scanning microscopy (CLSM). Methods: Forty human maxillary anterior teeth were instrumented and divided into four groups (n = 10) according to the drying methods: 1) wet: vacuum only, 2) paper points: vacuum + absorbent paper points, (3) 70% ethanol: 70% ethanol (1 min) + vacuum + absorbent paper points, and (4) 100% ethanol: 100% ethanol (1 min) + vacuum + absorbent paper points. All root canals were filled with resin-based endodontic sealer. Four sections from each third (cervical, middle, and apical) were examined by CLSM. Root canal wall perimeter infiltrated by sealer, maximum depth of sealer penetration, percentage of penetrated area, and fluorescence intensity of rhodamine B were measured. Statistical analysis was performed by analysis of variance and Tukey’s tests (α = 0.05). Results: No statistical difference was found when percentage of root canal wall coverage infiltrated by sealer were compared. The groups in which ethanol solutions were used presented greater depth of sealer penetration, higher percentage of penetrated area, and higher fluorescence intensity of rhodamine B (p< 0.05) when compared with the wet and paper point groups. Overall, 100% ethanol produced better results than 70% ethanol, except for rhodamine B intensity (cervical third). In addition, the absorbent paper points drying method behaved better than did vacuum only group, except for rhodamine B intensity (apical third). Conclusion: Ethanol-conditioned dentin improved the penetration of resin-based sealer into dentinal tubules, especially at the concentration of 100%.https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8661194Microscopy, confocalDentinEndodonticsEthanolWettabilityResin cements |
| spellingShingle | Carlos de Morais Souto Pantoja Diogo Henrique da Silva Adriana de Jesus Soares Caio Cezar Randi Ferraz Brenda Paula Figueiredo de Almeida Gomes Alexandre Augusto Zaia José Flávio Affonso de Almeida Effect of ethanol-conditioned dentine on sealer penetration into dentinal tubules Brazilian Journal of Oral Sciences Microscopy, confocal Dentin Endodontics Ethanol Wettability Resin cements |
| title | Effect of ethanol-conditioned dentine on sealer penetration into dentinal tubules |
| title_full | Effect of ethanol-conditioned dentine on sealer penetration into dentinal tubules |
| title_fullStr | Effect of ethanol-conditioned dentine on sealer penetration into dentinal tubules |
| title_full_unstemmed | Effect of ethanol-conditioned dentine on sealer penetration into dentinal tubules |
| title_short | Effect of ethanol-conditioned dentine on sealer penetration into dentinal tubules |
| title_sort | effect of ethanol conditioned dentine on sealer penetration into dentinal tubules |
| topic | Microscopy, confocal Dentin Endodontics Ethanol Wettability Resin cements |
| url | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8661194 |
| work_keys_str_mv | AT carlosdemoraissoutopantoja effectofethanolconditioneddentineonsealerpenetrationintodentinaltubules AT diogohenriquedasilva effectofethanolconditioneddentineonsealerpenetrationintodentinaltubules AT adrianadejesussoares effectofethanolconditioneddentineonsealerpenetrationintodentinaltubules AT caiocezarrandiferraz effectofethanolconditioneddentineonsealerpenetrationintodentinaltubules AT brendapaulafigueiredodealmeidagomes effectofethanolconditioneddentineonsealerpenetrationintodentinaltubules AT alexandreaugustozaia effectofethanolconditioneddentineonsealerpenetrationintodentinaltubules AT joseflavioaffonsodealmeida effectofethanolconditioneddentineonsealerpenetrationintodentinaltubules |