Efficacy of One-step Nucleic Acid Amplification Assay for Evaluation of Sentinel Lymph Node Metastasis of Breast Cancer in Songklanagarind Hospital
Objective: To compare the sensitivity, specificity and accuracy of the one-step nucleic acid amplification (OSNA) assay and frozen sections. Material and Method: One hundred and twenty patients with early breast cancer and no clinical nodal involvement underwent intraoperative sentinel lymph nodes (...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2017-01-01
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| Series: | Journal of Health Science and Medical Research (JHSMR) |
| Subjects: | |
| Online Access: | https://www.jhsmr.org/index.php/jhsmr/article/view/128 |
| _version_ | 1811340686437384192 |
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| author | Suphawat Laohawiriyakamol Puttisak Puttawibul Somrit Mahattanobon |
| author_facet | Suphawat Laohawiriyakamol Puttisak Puttawibul Somrit Mahattanobon |
| author_sort | Suphawat Laohawiriyakamol |
| collection | DOAJ |
| description | Objective: To compare the sensitivity, specificity and accuracy of the one-step nucleic acid amplification (OSNA) assay and frozen sections.
Material and Method: One hundred and twenty patients with early breast cancer and no clinical nodal involvement underwent intraoperative sentinel lymph nodes (SLNs) evaluation and undertaken axillary lymph nodes dissection (ALND) at the Department of Surgery, Songklanagarind Hospital from March 1st, 2011-November 30th, 2014.
Results: A total of 213 SLNs were evaluated (mean 1.84 nodes). The accuracy of the OSNA assay compared to histological evaluation was 95.7%. The OSNA assay performance was: sensitivity 100% (25/25); specificity 95.2% (179/188); positive predictive value (PPV) 73.5% (25/34) and negative predictive value (NPV) 100% (179/179). Turnaround time was an average 39.6 minutes. The accuracy of frozen sections compared to histological evaluation was 99.1%. The frozen sections performance was: sensitivity 92% (23/25); specificity 100% (188/188); PPV 100% (23/23) and NPV 98.9% (188/190). After discordant analysis, the PPV of the OSNA assay on nodes with macrometastases (++) was 100% (25/25), and the result on nodes with micrometastases (+) was 75% (3/4). The accuracy of OSNA after review was thus 99.5%.
Conclusion: The OSNA assay is accurate and rapid for SLN evaluation and it can replace the frozen section in general practice. |
| first_indexed | 2024-04-13T18:45:35Z |
| format | Article |
| id | doaj.art-bd42d69eef904c829ff8f30ac1018dbf |
| institution | Directory Open Access Journal |
| issn | 2586-9981 2630-0559 |
| language | English |
| last_indexed | 2024-04-13T18:45:35Z |
| publishDate | 2017-01-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Journal of Health Science and Medical Research (JHSMR) |
| spelling | doaj.art-bd42d69eef904c829ff8f30ac1018dbf2022-12-22T02:34:35ZengPrince of Songkla UniversityJournal of Health Science and Medical Research (JHSMR)2586-99812630-05592017-01-013511724142Efficacy of One-step Nucleic Acid Amplification Assay for Evaluation of Sentinel Lymph Node Metastasis of Breast Cancer in Songklanagarind HospitalSuphawat Laohawiriyakamol0Puttisak Puttawibul1Somrit Mahattanobon2Division of General Surgery, Department of Surgery, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110,Division of General Surgery, Department of Surgery, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110,Division of General Surgery, Department of Surgery, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110,Objective: To compare the sensitivity, specificity and accuracy of the one-step nucleic acid amplification (OSNA) assay and frozen sections. Material and Method: One hundred and twenty patients with early breast cancer and no clinical nodal involvement underwent intraoperative sentinel lymph nodes (SLNs) evaluation and undertaken axillary lymph nodes dissection (ALND) at the Department of Surgery, Songklanagarind Hospital from March 1st, 2011-November 30th, 2014. Results: A total of 213 SLNs were evaluated (mean 1.84 nodes). The accuracy of the OSNA assay compared to histological evaluation was 95.7%. The OSNA assay performance was: sensitivity 100% (25/25); specificity 95.2% (179/188); positive predictive value (PPV) 73.5% (25/34) and negative predictive value (NPV) 100% (179/179). Turnaround time was an average 39.6 minutes. The accuracy of frozen sections compared to histological evaluation was 99.1%. The frozen sections performance was: sensitivity 92% (23/25); specificity 100% (188/188); PPV 100% (23/23) and NPV 98.9% (188/190). After discordant analysis, the PPV of the OSNA assay on nodes with macrometastases (++) was 100% (25/25), and the result on nodes with micrometastases (+) was 75% (3/4). The accuracy of OSNA after review was thus 99.5%. Conclusion: The OSNA assay is accurate and rapid for SLN evaluation and it can replace the frozen section in general practice.https://www.jhsmr.org/index.php/jhsmr/article/view/128accuracyone-step nucleic acid amplificationosnasensitivityspecificity |
| spellingShingle | Suphawat Laohawiriyakamol Puttisak Puttawibul Somrit Mahattanobon Efficacy of One-step Nucleic Acid Amplification Assay for Evaluation of Sentinel Lymph Node Metastasis of Breast Cancer in Songklanagarind Hospital Journal of Health Science and Medical Research (JHSMR) accuracy one-step nucleic acid amplification osna sensitivity specificity |
| title | Efficacy of One-step Nucleic Acid Amplification Assay for Evaluation of Sentinel Lymph Node Metastasis of Breast Cancer in Songklanagarind Hospital |
| title_full | Efficacy of One-step Nucleic Acid Amplification Assay for Evaluation of Sentinel Lymph Node Metastasis of Breast Cancer in Songklanagarind Hospital |
| title_fullStr | Efficacy of One-step Nucleic Acid Amplification Assay for Evaluation of Sentinel Lymph Node Metastasis of Breast Cancer in Songklanagarind Hospital |
| title_full_unstemmed | Efficacy of One-step Nucleic Acid Amplification Assay for Evaluation of Sentinel Lymph Node Metastasis of Breast Cancer in Songklanagarind Hospital |
| title_short | Efficacy of One-step Nucleic Acid Amplification Assay for Evaluation of Sentinel Lymph Node Metastasis of Breast Cancer in Songklanagarind Hospital |
| title_sort | efficacy of one step nucleic acid amplification assay for evaluation of sentinel lymph node metastasis of breast cancer in songklanagarind hospital |
| topic | accuracy one-step nucleic acid amplification osna sensitivity specificity |
| url | https://www.jhsmr.org/index.php/jhsmr/article/view/128 |
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