Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish
Obesity is a worldwide public health problem with increasing prevalence and affects 80% of diabetes mellitus type 2 cases. Zebrafish (<i>Danio rerio</i>) is an established model organism for studying obesity and diabetes including diabetic microvascular complications. We aimed to determi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/10/1581 |
| _version_ | 1797515449161744384 |
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| author | Franziska J. Heinkele Bowen Lou Vanessa Erben Katrin Bennewitz Gernot Poschet Carsten Sticht Jens Kroll |
| author_facet | Franziska J. Heinkele Bowen Lou Vanessa Erben Katrin Bennewitz Gernot Poschet Carsten Sticht Jens Kroll |
| author_sort | Franziska J. Heinkele |
| collection | DOAJ |
| description | Obesity is a worldwide public health problem with increasing prevalence and affects 80% of diabetes mellitus type 2 cases. Zebrafish (<i>Danio rerio</i>) is an established model organism for studying obesity and diabetes including diabetic microvascular complications. We aimed to determine whether physical activity is an appropriate tool to examine training effects in zebrafish and to analyse metabolic and transcriptional processes in trained zebrafish. A 2- and 8-week experimental training phase protocol with adult zebrafish in a swim tunnel system was established. We examined zebrafish basic characteristics before and after training such as body weight, body length and maximum speed and considered overfeeding as an additional parameter in the 8-weeks training protocol. Ultimately, the effects of training and overfeeding on blood glucose, muscle core metabolism and liver gene expression using RNA-Seq were investigated. Zebrafish maximum speed was correlated with body length and was significantly increased after 2 weeks of training. Maximum swim speed further increased after 8 weeks of training in both the normal-fed and the overfed groups, but training was found not to be sufficient in preventing weight gain in overfed fish. Metabolome and transcriptome profiling in trained fish exhibited increased blood glucose levels in the short-term and upregulated energy supply pathways as well as response to oxidative stress in the long-term. In conclusion, swim training is a valuable tool to study the effects of physical activity in zebrafish, which is accompanied by metabolic and transcriptional adaptations. |
| first_indexed | 2024-03-10T06:45:38Z |
| format | Article |
| id | doaj.art-bd4baf6049b74645af9628168b106a19 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T06:45:38Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-bd4baf6049b74645af9628168b106a192023-11-22T17:16:13ZengMDPI AGAntioxidants2076-39212021-10-011010158110.3390/antiox10101581Metabolic and Transcriptional Adaptations Improve Physical Performance of ZebrafishFranziska J. Heinkele0Bowen Lou1Vanessa Erben2Katrin Bennewitz3Gernot Poschet4Carsten Sticht5Jens Kroll6Department of Vascular Biology and Tumor Angiogenesis, European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, D-68167 Mannheim, GermanyDepartment of Vascular Biology and Tumor Angiogenesis, European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, D-68167 Mannheim, GermanyDepartment of Vascular Biology and Tumor Angiogenesis, European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, D-68167 Mannheim, GermanyDepartment of Vascular Biology and Tumor Angiogenesis, European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, D-68167 Mannheim, GermanyMetabolomics Core Technology Platform, Centre for Organismal Studies, Heidelberg University, D-69120 Heidelberg, GermanyNGS Core Facility, Medical Faculty Mannheim, Heidelberg University, D-68167 Mannheim, GermanyDepartment of Vascular Biology and Tumor Angiogenesis, European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, D-68167 Mannheim, GermanyObesity is a worldwide public health problem with increasing prevalence and affects 80% of diabetes mellitus type 2 cases. Zebrafish (<i>Danio rerio</i>) is an established model organism for studying obesity and diabetes including diabetic microvascular complications. We aimed to determine whether physical activity is an appropriate tool to examine training effects in zebrafish and to analyse metabolic and transcriptional processes in trained zebrafish. A 2- and 8-week experimental training phase protocol with adult zebrafish in a swim tunnel system was established. We examined zebrafish basic characteristics before and after training such as body weight, body length and maximum speed and considered overfeeding as an additional parameter in the 8-weeks training protocol. Ultimately, the effects of training and overfeeding on blood glucose, muscle core metabolism and liver gene expression using RNA-Seq were investigated. Zebrafish maximum speed was correlated with body length and was significantly increased after 2 weeks of training. Maximum swim speed further increased after 8 weeks of training in both the normal-fed and the overfed groups, but training was found not to be sufficient in preventing weight gain in overfed fish. Metabolome and transcriptome profiling in trained fish exhibited increased blood glucose levels in the short-term and upregulated energy supply pathways as well as response to oxidative stress in the long-term. In conclusion, swim training is a valuable tool to study the effects of physical activity in zebrafish, which is accompanied by metabolic and transcriptional adaptations.https://www.mdpi.com/2076-3921/10/10/1581danio reriodiabetesphysical activityblood glucosemetabolomicstranscriptional profile |
| spellingShingle | Franziska J. Heinkele Bowen Lou Vanessa Erben Katrin Bennewitz Gernot Poschet Carsten Sticht Jens Kroll Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish Antioxidants danio rerio diabetes physical activity blood glucose metabolomics transcriptional profile |
| title | Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish |
| title_full | Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish |
| title_fullStr | Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish |
| title_full_unstemmed | Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish |
| title_short | Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish |
| title_sort | metabolic and transcriptional adaptations improve physical performance of zebrafish |
| topic | danio rerio diabetes physical activity blood glucose metabolomics transcriptional profile |
| url | https://www.mdpi.com/2076-3921/10/10/1581 |
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