Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study
Abstract Background Indoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplac...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-023-02951-5 |
| _version_ | 1797752858946306048 |
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| author | Eva Konecna Petra Videnska Lucie Buresova Milan Urik Sona Smetanova Stanislav Smatana Roman Prokes Barbara Lanickova Eva Budinska Jana Klanova Petra Borilova Linhartova |
| author_facet | Eva Konecna Petra Videnska Lucie Buresova Milan Urik Sona Smetanova Stanislav Smatana Roman Prokes Barbara Lanickova Eva Budinska Jana Klanova Petra Borilova Linhartova |
| author_sort | Eva Konecna |
| collection | DOAJ |
| description | Abstract Background Indoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplace) environments. Methods In this pilot study, nasopharyngeal swabs were taken from 22 participants in the morning and after 8 h of their presence at the workplace. At the same time points, indoor dust samples were collected from the participants’ households (16 from flats and 6 from houses) and workplaces (8 from a maternity hospital – NEO, 6 from a pediatric hospital – ENT, and 8 from a research center – RCX). 16S rRNA sequencing analysis was performed on these human and environmental matrices. Results Staphylococcus and Corynebacterium were the most abundant genera in both indoor dust and nasopharyngeal samples. The analysis indicated lower bacterial diversity in indoor dust samples from flats compared to houses, NEO, ENT, and RCX (p < 0.05). Participants working in the NEO had the highest nasopharyngeal bacterial diversity of all groups (p < 0.05). After 8 h of exposure to the workplace environment, enrichment of the nasopharynx with several new bacterial genera present in the indoor dust was observed in 76% of study participants; however, no significant changes were observed at the level of the nasopharyngeal bacterial diversity (p > 0.05, Shannon index). These “enriching” bacterial genera overlapped between the hospital workplaces – NEO and ENT but differed from those in the research center – RCX. Conclusions The results suggest that although the composition of nasopharyngeal bacteriome is relatively stable during the day. Short-term exposure to the indoor environment can result in the enrichment of the nasopharynx with bacterial DNA from indoor dust; the bacterial composition, however, varies by the indoor workplace environment. |
| first_indexed | 2024-03-12T17:09:33Z |
| format | Article |
| id | doaj.art-bd508d9352cf4df98d051bf9173bd943 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-12T17:09:33Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-bd508d9352cf4df98d051bf9173bd9432023-08-06T11:08:29ZengBMCBMC Microbiology1471-21802023-07-0123111610.1186/s12866-023-02951-5Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot studyEva Konecna0Petra Videnska1Lucie Buresova2Milan Urik3Sona Smetanova4Stanislav Smatana5Roman Prokes6Barbara Lanickova7Eva Budinska8Jana Klanova9Petra Borilova Linhartova10RECETOX, Faculty of Science, Masaryk UniversityRECETOX, Faculty of Science, Masaryk UniversityRECETOX, Faculty of Science, Masaryk UniversityDepartment of Pediatric Otorhinolaryngology, University Hospital BrnoRECETOX, Faculty of Science, Masaryk UniversityRECETOX, Faculty of Science, Masaryk UniversityRECETOX, Faculty of Science, Masaryk UniversityRECETOX, Faculty of Science, Masaryk UniversityRECETOX, Faculty of Science, Masaryk UniversityRECETOX, Faculty of Science, Masaryk UniversityRECETOX, Faculty of Science, Masaryk UniversityAbstract Background Indoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplace) environments. Methods In this pilot study, nasopharyngeal swabs were taken from 22 participants in the morning and after 8 h of their presence at the workplace. At the same time points, indoor dust samples were collected from the participants’ households (16 from flats and 6 from houses) and workplaces (8 from a maternity hospital – NEO, 6 from a pediatric hospital – ENT, and 8 from a research center – RCX). 16S rRNA sequencing analysis was performed on these human and environmental matrices. Results Staphylococcus and Corynebacterium were the most abundant genera in both indoor dust and nasopharyngeal samples. The analysis indicated lower bacterial diversity in indoor dust samples from flats compared to houses, NEO, ENT, and RCX (p < 0.05). Participants working in the NEO had the highest nasopharyngeal bacterial diversity of all groups (p < 0.05). After 8 h of exposure to the workplace environment, enrichment of the nasopharynx with several new bacterial genera present in the indoor dust was observed in 76% of study participants; however, no significant changes were observed at the level of the nasopharyngeal bacterial diversity (p > 0.05, Shannon index). These “enriching” bacterial genera overlapped between the hospital workplaces – NEO and ENT but differed from those in the research center – RCX. Conclusions The results suggest that although the composition of nasopharyngeal bacteriome is relatively stable during the day. Short-term exposure to the indoor environment can result in the enrichment of the nasopharynx with bacterial DNA from indoor dust; the bacterial composition, however, varies by the indoor workplace environment.https://doi.org/10.1186/s12866-023-02951-5BacteriomeDustNasopharynxHouseholdHospitalSequencing |
| spellingShingle | Eva Konecna Petra Videnska Lucie Buresova Milan Urik Sona Smetanova Stanislav Smatana Roman Prokes Barbara Lanickova Eva Budinska Jana Klanova Petra Borilova Linhartova Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study BMC Microbiology Bacteriome Dust Nasopharynx Household Hospital Sequencing |
| title | Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study |
| title_full | Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study |
| title_fullStr | Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study |
| title_full_unstemmed | Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study |
| title_short | Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study |
| title_sort | enrichment of human nasopharyngeal bacteriome with bacteria from dust after short term exposure to indoor environment a pilot study |
| topic | Bacteriome Dust Nasopharynx Household Hospital Sequencing |
| url | https://doi.org/10.1186/s12866-023-02951-5 |
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