Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry
Cervicitis is an exceedingly common gynecological disorder that puts women at high risk of sexually transmitted infections and induces a series of reproductive system diseases. This condition also has a significant impact on quality of life and is commonly misdiagnosed in clinical practice due to it...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2018.00282/full |
| _version_ | 1828735511307485184 |
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| author | Xiaoyong Zhang Junmao Li Junmao Li Bin Xie Bei Wu Shuangxia Lei Yun Yao Mingzhen He Mingzhen He Hui Ouyang Yulin Feng Yulin Feng Wen Xu Shilin Yang Shilin Yang |
| author_facet | Xiaoyong Zhang Junmao Li Junmao Li Bin Xie Bei Wu Shuangxia Lei Yun Yao Mingzhen He Mingzhen He Hui Ouyang Yulin Feng Yulin Feng Wen Xu Shilin Yang Shilin Yang |
| author_sort | Xiaoyong Zhang |
| collection | DOAJ |
| description | Cervicitis is an exceedingly common gynecological disorder that puts women at high risk of sexually transmitted infections and induces a series of reproductive system diseases. This condition also has a significant impact on quality of life and is commonly misdiagnosed in clinical practice due to its complicated pathogenesis. In the present study, we performed non-targeted plasma metabolomics analysis of cervicitis in both plasma samples obtained from human patients and plasma samples from a phenol mucilage induced rat model of cervicitis, using ultra-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry. In addition to differences in histopathology, we identified differences in the metabolic profile between the cervicitis and control groups using unsupervised principal component analysis and orthogonal projections to latent structures discriminant analysis. These results demonstrated changes in plasma metabolites, with 27 and 22 potential endogenous markers identified in rat and human samples, respectively. The metabolic pathway analysis showed that linoleic acid, arachidonic acid, ether lipid, and glycerophospholipid metabolism are key metabolic pathways involved in cervicitis. This study showed the rat model was successfully created and applied to understand the pathogenesis of cervicitis. |
| first_indexed | 2024-04-12T23:08:20Z |
| format | Article |
| id | doaj.art-bd51ad2c9d3c4e22995ca7c74e679339 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-12T23:08:20Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-bd51ad2c9d3c4e22995ca7c74e6793392022-12-22T03:12:52ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-04-01910.3389/fphar.2018.00282350922Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass SpectrometryXiaoyong Zhang0Junmao Li1Junmao Li2Bin Xie3Bei Wu4Shuangxia Lei5Yun Yao6Mingzhen He7Mingzhen He8Hui Ouyang9Yulin Feng10Yulin Feng11Wen Xu12Shilin Yang13Shilin Yang14Jiangxi University of Traditional Chinese Medicine, Nanchang, ChinaJiangxi University of Traditional Chinese Medicine, Nanchang, ChinaState Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, ChinaJiangxi University of Traditional Chinese Medicine, Nanchang, ChinaNanchang Institute for Food and Drug Control, Nanchang, ChinaJiangxi University of Traditional Chinese Medicine, Nanchang, ChinaJiangxi University of Traditional Chinese Medicine, Nanchang, ChinaJiangxi University of Traditional Chinese Medicine, Nanchang, ChinaState Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, ChinaJiangxi University of Traditional Chinese Medicine, Nanchang, ChinaJiangxi University of Traditional Chinese Medicine, Nanchang, ChinaState Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, ChinaSecond College of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, ChinaJiangxi University of Traditional Chinese Medicine, Nanchang, ChinaState Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, ChinaCervicitis is an exceedingly common gynecological disorder that puts women at high risk of sexually transmitted infections and induces a series of reproductive system diseases. This condition also has a significant impact on quality of life and is commonly misdiagnosed in clinical practice due to its complicated pathogenesis. In the present study, we performed non-targeted plasma metabolomics analysis of cervicitis in both plasma samples obtained from human patients and plasma samples from a phenol mucilage induced rat model of cervicitis, using ultra-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry. In addition to differences in histopathology, we identified differences in the metabolic profile between the cervicitis and control groups using unsupervised principal component analysis and orthogonal projections to latent structures discriminant analysis. These results demonstrated changes in plasma metabolites, with 27 and 22 potential endogenous markers identified in rat and human samples, respectively. The metabolic pathway analysis showed that linoleic acid, arachidonic acid, ether lipid, and glycerophospholipid metabolism are key metabolic pathways involved in cervicitis. This study showed the rat model was successfully created and applied to understand the pathogenesis of cervicitis.http://journal.frontiersin.org/article/10.3389/fphar.2018.00282/fullcervicitisUPLC-QTOF-MS/MSmetabolomicsmetabolic pathwaymultivariate analysis |
| spellingShingle | Xiaoyong Zhang Junmao Li Junmao Li Bin Xie Bei Wu Shuangxia Lei Yun Yao Mingzhen He Mingzhen He Hui Ouyang Yulin Feng Yulin Feng Wen Xu Shilin Yang Shilin Yang Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry Frontiers in Pharmacology cervicitis UPLC-QTOF-MS/MS metabolomics metabolic pathway multivariate analysis |
| title | Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry |
| title_full | Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry |
| title_fullStr | Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry |
| title_full_unstemmed | Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry |
| title_short | Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry |
| title_sort | comparative metabolomics analysis of cervicitis in human patients and a phenol mucilage induced rat model using liquid chromatography tandem mass spectrometry |
| topic | cervicitis UPLC-QTOF-MS/MS metabolomics metabolic pathway multivariate analysis |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2018.00282/full |
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