Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment
Summary: The nematode Caenorhabditis elegans has been developed as a valuable genetic model for research on aging and aging-related diseases. Here, we present a protocol for assessing the healthspan of C. elegans after treatment of a potential anti-aging drug. We describe steps for C. elegans synchr...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723002526 |
| _version_ | 1797832313154830336 |
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| author | Yi Xiao Li Zhang Yun Liu |
| author_facet | Yi Xiao Li Zhang Yun Liu |
| author_sort | Yi Xiao |
| collection | DOAJ |
| description | Summary: The nematode Caenorhabditis elegans has been developed as a valuable genetic model for research on aging and aging-related diseases. Here, we present a protocol for assessing the healthspan of C. elegans after treatment of a potential anti-aging drug. We describe steps for C. elegans synchronization, drug treatment, and lifespan determination from the survivorship curve. We also detail assessment of locomotory ability through body bend rate and measurement of lipofuscin fluorescence to quantify age pigment in the worm intestine.For complete details on the use and execution of this protocol, please refer to Xiao et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-09T14:05:49Z |
| format | Article |
| id | doaj.art-bd52d43471a74cc7a87028c6ffc9935b |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-09T14:05:49Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-bd52d43471a74cc7a87028c6ffc9935b2023-05-07T04:17:42ZengElsevierSTAR Protocols2666-16672023-06-0142102285Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatmentYi Xiao0Li Zhang1Yun Liu2Guizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines, Zunyi Medical University, Zunyi, Guizhou 563000, China; College of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou 563000, China; Corresponding authorGuizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines, Zunyi Medical University, Zunyi, Guizhou 563000, China; College of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou 563000, ChinaGuizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines, Zunyi Medical University, Zunyi, Guizhou 563000, China; College of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou 563000, China; Corresponding authorSummary: The nematode Caenorhabditis elegans has been developed as a valuable genetic model for research on aging and aging-related diseases. Here, we present a protocol for assessing the healthspan of C. elegans after treatment of a potential anti-aging drug. We describe steps for C. elegans synchronization, drug treatment, and lifespan determination from the survivorship curve. We also detail assessment of locomotory ability through body bend rate and measurement of lipofuscin fluorescence to quantify age pigment in the worm intestine.For complete details on the use and execution of this protocol, please refer to Xiao et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723002526Health SciencesMicrobiologyMicroscopyModel Organisms |
| spellingShingle | Yi Xiao Li Zhang Yun Liu Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment STAR Protocols Health Sciences Microbiology Microscopy Model Organisms |
| title | Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment |
| title_full | Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment |
| title_fullStr | Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment |
| title_full_unstemmed | Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment |
| title_short | Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment |
| title_sort | protocol for assessing the healthspan of caenorhabditis elegans after potential anti aging drug treatment |
| topic | Health Sciences Microbiology Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723002526 |
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