Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification

Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification

Abstract Background 5-Aminolevulinic acid (ALA) recently received much attention due to its potential application in many fields. In this study, an ALA production strain of Escherichia coli was constructed by rational metabolic engineering and stepwise improvement based on known regulatory and metab...

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Bibliographic Details
Main Authors: Changchuan Ye, Yuting Yang, Xi Chen, Lijie Yang, Xia Hua, Mengjie Yang, Xiangfang Zeng, Shiyan Qiao
Format: Article
Language:English
Published: BMC 2022-10-01
Series:Journal of Biological Engineering
Subjects:
E. coli
5-Aminolevulinic Acid
CRISPR/cas9
T7 Expression System
Metabolic engineering
Online Access:https://doi.org/10.1186/s13036-022-00307-7

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