Cytomorphometry of normal exfoliated gingival cells: An analytical study
Introduction: Gingiva is lined by stratified squamous epithelium. One of the most common types of carcinoma in the world is oral cancer. Gingival cancers are a common type of oral neoplasm. In order to compare normal tissue with pathological smears using exfoliative oral cytology with morphometry,...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | National Journal of Clinical Anatomy |
| Subjects: | |
| Online Access: | http://www.njca.info/article.asp?issn=2277-4025;year=2023;volume=12;issue=2;spage=87;epage=93;aulast=Patel |
| _version_ | 1827894702758690816 |
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| author | Ridhdhish Kantilal Patel Ritika Mohanbhai Patel Rashvaita Kantilal Patel |
| author_facet | Ridhdhish Kantilal Patel Ritika Mohanbhai Patel Rashvaita Kantilal Patel |
| author_sort | Ridhdhish Kantilal Patel |
| collection | DOAJ |
| description | Introduction: Gingiva is lined by stratified squamous epithelium. One of the most common types of carcinoma in the world is oral cancer. Gingival cancers are a common type of oral neoplasm. In order to compare normal tissue with pathological smears using exfoliative oral cytology with morphometry, a baseline must be established. A study was conducted to obtain nuclear area (NA), cytoplasmic area (CA), and N:C ratio-nuclear: Cytoplasmic ratio of exfoliated normal gingival cells and to correlate it with healthy individuals by their age and gender. Methodology: Exfoliated gingival squamous cell smears were obtained from 200 subjects (100 males, 100 females) belonging to age groups <20 years, 21–40 years, 41–60 years, and >60 years. Smears were fixed and stained by routine Hematoxylin and Eosin staining procedure. With the use of both ocular micrometer and stage micrometer different cellular parameters were measured. Student's t-test was used for statistical analysis. Results: There were significant differences in NA, CA, and N:C ratio with age regardless of gender. Different age groups of males showed significant variations (P < 0.0001) in all the above parameters. The same was true for different age groups of females. In terms of NA, CA, and N:C ratio, the difference between both genders was significant regardless of age. Conclusion: Gingival smears showed age and sex-linked changes in NA, CA, and nuclear: Cytoplasmic ratio. It might serve as a standard for these variables when comparing similar measurements based on pathologic smears of malignant or premalignant nature. |
| first_indexed | 2024-03-12T22:10:18Z |
| format | Article |
| id | doaj.art-bd565ac98c6d4ce99629fb198191d3e6 |
| institution | Directory Open Access Journal |
| issn | 2277-4025 2321-2780 |
| language | English |
| last_indexed | 2024-03-12T22:10:18Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | National Journal of Clinical Anatomy |
| spelling | doaj.art-bd565ac98c6d4ce99629fb198191d3e62023-07-23T16:10:22ZengWolters Kluwer Medknow PublicationsNational Journal of Clinical Anatomy2277-40252321-27802023-01-01122879310.4103/NJCA.NJCA_245_22Cytomorphometry of normal exfoliated gingival cells: An analytical studyRidhdhish Kantilal PatelRitika Mohanbhai PatelRashvaita Kantilal PatelIntroduction: Gingiva is lined by stratified squamous epithelium. One of the most common types of carcinoma in the world is oral cancer. Gingival cancers are a common type of oral neoplasm. In order to compare normal tissue with pathological smears using exfoliative oral cytology with morphometry, a baseline must be established. A study was conducted to obtain nuclear area (NA), cytoplasmic area (CA), and N:C ratio-nuclear: Cytoplasmic ratio of exfoliated normal gingival cells and to correlate it with healthy individuals by their age and gender. Methodology: Exfoliated gingival squamous cell smears were obtained from 200 subjects (100 males, 100 females) belonging to age groups <20 years, 21–40 years, 41–60 years, and >60 years. Smears were fixed and stained by routine Hematoxylin and Eosin staining procedure. With the use of both ocular micrometer and stage micrometer different cellular parameters were measured. Student's t-test was used for statistical analysis. Results: There were significant differences in NA, CA, and N:C ratio with age regardless of gender. Different age groups of males showed significant variations (P < 0.0001) in all the above parameters. The same was true for different age groups of females. In terms of NA, CA, and N:C ratio, the difference between both genders was significant regardless of age. Conclusion: Gingival smears showed age and sex-linked changes in NA, CA, and nuclear: Cytoplasmic ratio. It might serve as a standard for these variables when comparing similar measurements based on pathologic smears of malignant or premalignant nature.http://www.njca.info/article.asp?issn=2277-4025;year=2023;volume=12;issue=2;spage=87;epage=93;aulast=Patelbaselinecytomorphometrycytoplasmic areagingival smearnuclear areanuclear: cytoplasmic ratiooral cancer |
| spellingShingle | Ridhdhish Kantilal Patel Ritika Mohanbhai Patel Rashvaita Kantilal Patel Cytomorphometry of normal exfoliated gingival cells: An analytical study National Journal of Clinical Anatomy baseline cytomorphometry cytoplasmic area gingival smear nuclear area nuclear: cytoplasmic ratio oral cancer |
| title | Cytomorphometry of normal exfoliated gingival cells: An analytical study |
| title_full | Cytomorphometry of normal exfoliated gingival cells: An analytical study |
| title_fullStr | Cytomorphometry of normal exfoliated gingival cells: An analytical study |
| title_full_unstemmed | Cytomorphometry of normal exfoliated gingival cells: An analytical study |
| title_short | Cytomorphometry of normal exfoliated gingival cells: An analytical study |
| title_sort | cytomorphometry of normal exfoliated gingival cells an analytical study |
| topic | baseline cytomorphometry cytoplasmic area gingival smear nuclear area nuclear: cytoplasmic ratio oral cancer |
| url | http://www.njca.info/article.asp?issn=2277-4025;year=2023;volume=12;issue=2;spage=87;epage=93;aulast=Patel |
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