Evaluation of P53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methods
Abstract Objective Periodontitis is one of the most important periodontal diseases that can be affected by many factors. Although the mechanism of periodontitis development is not yet fully understood, previous studies suggest that apoptosis may be one of the pathological factors that can affect the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-12-01
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| Series: | Clinical and Experimental Dental Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cre2.668 |
| _version_ | 1811291348902346752 |
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| author | Samaneh Minabian Shima Soleimani S. Molook Torabi Mohammad Mohammadi Hadi Ranjbar |
| author_facet | Samaneh Minabian Shima Soleimani S. Molook Torabi Mohammad Mohammadi Hadi Ranjbar |
| author_sort | Samaneh Minabian |
| collection | DOAJ |
| description | Abstract Objective Periodontitis is one of the most important periodontal diseases that can be affected by many factors. Although the mechanism of periodontitis development is not yet fully understood, previous studies suggest that apoptosis may be one of the pathological factors that can affect the process of the disease by destroying old and damaged cells. Low expression of P53 protein is one of the reasons for delaying cell death that allows damaged cells to survive longer and gives more time for the chance of mutations and pathogenesis. Because of the important role of P53 in gingival cells of patients with chronic periodontitis, the objective of our study is to evaluate the P53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methods. Materials and Methods In this cross‐sectional study, 35 patients with severe to moderate chronic periodontitis (loss of attachment ≥3 mm, probing depth ≥5 mm) with no treatment and 25 people who were healthy for periodontal problems were examined. Gingival biopsies from marginal and attached gingiva were obtained, prepared, and mounted on slides. Then, the expression of P53 on each slide was evaluated by optic microscopy after using P53 antibodies and staining with hematoxylin‐eosin (immunohistochemistry method). Data were analyzed using independent t‐test, Mann–Whitney U‐test, and Spearman correlation test using SPSS Statistics version 18.0. Results The mean ages of participants in the case and control groups were 37.58 and 32.09, respectively. Our results showed that the expression of P53 was not significant in periodontitis compared to the control group (p > .05). Also, gender could not affect the expression of P53 in both groups (p > .05), and there was no significant relationship between age and P53 gene incidence. Conclusion Chronic periodontitis has no significant effect on P53 expression, so changes in apoptosis due to P53 expression in periodontitis are not significant. |
| first_indexed | 2024-04-13T04:27:56Z |
| format | Article |
| id | doaj.art-bd5885c2a830404eb38bc32bf011eeb5 |
| institution | Directory Open Access Journal |
| issn | 2057-4347 |
| language | English |
| last_indexed | 2024-04-13T04:27:56Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Experimental Dental Research |
| spelling | doaj.art-bd5885c2a830404eb38bc32bf011eeb52022-12-22T03:02:27ZengWileyClinical and Experimental Dental Research2057-43472022-12-01861348135310.1002/cre2.668Evaluation of P53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methodsSamaneh Minabian0Shima Soleimani S.1Molook Torabi2Mohammad Mohammadi3Hadi Ranjbar4Oral and Dental Diseases Research Center Kerman University of Medical Sciences Kerman IranOral and Dental Diseases Research Center Kerman University of Medical Sciences Kerman IranOral and Dental Diseases Research Center Kerman University of Medical Sciences Kerman IranOral and Dental Diseases Research Center Kerman University of Medical Sciences Kerman IranMental Health Research Center, Psychological Health Research Institute Iran University of Medical Sciences Tehran IranAbstract Objective Periodontitis is one of the most important periodontal diseases that can be affected by many factors. Although the mechanism of periodontitis development is not yet fully understood, previous studies suggest that apoptosis may be one of the pathological factors that can affect the process of the disease by destroying old and damaged cells. Low expression of P53 protein is one of the reasons for delaying cell death that allows damaged cells to survive longer and gives more time for the chance of mutations and pathogenesis. Because of the important role of P53 in gingival cells of patients with chronic periodontitis, the objective of our study is to evaluate the P53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methods. Materials and Methods In this cross‐sectional study, 35 patients with severe to moderate chronic periodontitis (loss of attachment ≥3 mm, probing depth ≥5 mm) with no treatment and 25 people who were healthy for periodontal problems were examined. Gingival biopsies from marginal and attached gingiva were obtained, prepared, and mounted on slides. Then, the expression of P53 on each slide was evaluated by optic microscopy after using P53 antibodies and staining with hematoxylin‐eosin (immunohistochemistry method). Data were analyzed using independent t‐test, Mann–Whitney U‐test, and Spearman correlation test using SPSS Statistics version 18.0. Results The mean ages of participants in the case and control groups were 37.58 and 32.09, respectively. Our results showed that the expression of P53 was not significant in periodontitis compared to the control group (p > .05). Also, gender could not affect the expression of P53 in both groups (p > .05), and there was no significant relationship between age and P53 gene incidence. Conclusion Chronic periodontitis has no significant effect on P53 expression, so changes in apoptosis due to P53 expression in periodontitis are not significant.https://doi.org/10.1002/cre2.668apoptosischronic periodontitisexpressionP53 |
| spellingShingle | Samaneh Minabian Shima Soleimani S. Molook Torabi Mohammad Mohammadi Hadi Ranjbar Evaluation of P53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methods Clinical and Experimental Dental Research apoptosis chronic periodontitis expression P53 |
| title | Evaluation of P53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methods |
| title_full | Evaluation of P53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methods |
| title_fullStr | Evaluation of P53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methods |
| title_full_unstemmed | Evaluation of P53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methods |
| title_short | Evaluation of P53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methods |
| title_sort | evaluation of p53 protein expression in gingival tissues of patients with chronic periodontitis by immunohistochemistry methods |
| topic | apoptosis chronic periodontitis expression P53 |
| url | https://doi.org/10.1002/cre2.668 |
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