Optimization and Comparison of Ultrasound and Microwave-Assisted Extraction of Phenolic Compounds from Cotton-Lavender (<i>Santolina chamaecyparissus</i> L.)
The interest in natural phenolic compounds has increased because of their attractive use especially as antioxidant and antimicrobial agents in foods. The large content in phenolic compounds of interest in <i>Santolina chamaecyparissus</i> L. (<i>S. chamaecyparissus</i>) makes...
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MDPI AG
2021-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/1/84 |
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| author | Mohammed Aourach Ana V. González-de-Peredo Mercedes Vázquez-Espinosa Haiat Essalmani Miguel Palma Gerardo F. Barbero |
| author_facet | Mohammed Aourach Ana V. González-de-Peredo Mercedes Vázquez-Espinosa Haiat Essalmani Miguel Palma Gerardo F. Barbero |
| author_sort | Mohammed Aourach |
| collection | DOAJ |
| description | The interest in natural phenolic compounds has increased because of their attractive use especially as antioxidant and antimicrobial agents in foods. The large content in phenolic compounds of interest in <i>Santolina chamaecyparissus</i> L. (<i>S. chamaecyparissus</i>) makes this plant a target source that is worthy of note. In this work, new extraction technologies comprising ultrasound (UAE) and microwave (MAE) assisted extraction of the phenolic compounds in <i>S. chamaecyparissus</i> have been developed, optimized, and compared. Several extraction factors have been optimized based on a Box-Behnken design. Such optimized factors include the percentage of methanol in water (25–75%), the temperature (10–70 °C), the ultrasound amplitude (20–80%), the ultrasound cycle (0.2–1 s), the solvent pH (2–7) and the solvent-sample ratio (5/0.2–15/0.2 mL/g) with regard to UAE, while the percentage of methanol in water (50–100%), the temperature (50–100 °C), the pH (2–7) and the solvent-sample ratio (5/0.2–15/0.2 mL/g) were optimized for MAE. The solvent composition was the most influential parameter both on MAEs (64%) and UAEs (74%). The extraction optimum time was established as 15 min for MAE and 25 min for UAE. Five major phenolic compounds were detected and identified by Ultra-High-Performance Liquid Chromatography—Quadrupole Time of Flight—Mass Spectrometry (UHPLC-QToF-MS) in the extracts: chlorogenic acid, quercetin 3-<i>O</i>-galactoside, quercetin 3-<i>O</i>-glucoside, isoorientin, and cynarin. With the exception of chlorogenic acid, the other four compounds have been identified for the first time in <i>S. chamaecyparissus</i>. The findings have confirmed that MAE is a significantly more efficient extraction method than UAE to extract phenolic compounds from <i>S. chamaecyparissus.</i> |
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| language | English |
| last_indexed | 2024-03-10T13:30:19Z |
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| spelling | doaj.art-bd593372552e436fa055b99a8a29041d2023-11-21T08:05:05ZengMDPI AGAgronomy2073-43952021-01-011118410.3390/agronomy11010084Optimization and Comparison of Ultrasound and Microwave-Assisted Extraction of Phenolic Compounds from Cotton-Lavender (<i>Santolina chamaecyparissus</i> L.)Mohammed Aourach0Ana V. González-de-Peredo1Mercedes Vázquez-Espinosa2Haiat Essalmani3Miguel Palma4Gerardo F. Barbero5Team of Biotechnological Valorization of Microorganism, Genomics and Bioinformatics, Department of Biology, Faculty of Sciences and Techniques, University Abdelmalek Essaâdi, B.P. 415 Tangier, MoroccoDepartment of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, 11510 Puerto Real, Cadiz, SpainDepartment of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, 11510 Puerto Real, Cadiz, SpainTeam of Biotechnological Valorization of Microorganism, Genomics and Bioinformatics, Department of Biology, Faculty of Sciences and Techniques, University Abdelmalek Essaâdi, B.P. 415 Tangier, MoroccoDepartment of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, 11510 Puerto Real, Cadiz, SpainDepartment of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, 11510 Puerto Real, Cadiz, SpainThe interest in natural phenolic compounds has increased because of their attractive use especially as antioxidant and antimicrobial agents in foods. The large content in phenolic compounds of interest in <i>Santolina chamaecyparissus</i> L. (<i>S. chamaecyparissus</i>) makes this plant a target source that is worthy of note. In this work, new extraction technologies comprising ultrasound (UAE) and microwave (MAE) assisted extraction of the phenolic compounds in <i>S. chamaecyparissus</i> have been developed, optimized, and compared. Several extraction factors have been optimized based on a Box-Behnken design. Such optimized factors include the percentage of methanol in water (25–75%), the temperature (10–70 °C), the ultrasound amplitude (20–80%), the ultrasound cycle (0.2–1 s), the solvent pH (2–7) and the solvent-sample ratio (5/0.2–15/0.2 mL/g) with regard to UAE, while the percentage of methanol in water (50–100%), the temperature (50–100 °C), the pH (2–7) and the solvent-sample ratio (5/0.2–15/0.2 mL/g) were optimized for MAE. The solvent composition was the most influential parameter both on MAEs (64%) and UAEs (74%). The extraction optimum time was established as 15 min for MAE and 25 min for UAE. Five major phenolic compounds were detected and identified by Ultra-High-Performance Liquid Chromatography—Quadrupole Time of Flight—Mass Spectrometry (UHPLC-QToF-MS) in the extracts: chlorogenic acid, quercetin 3-<i>O</i>-galactoside, quercetin 3-<i>O</i>-glucoside, isoorientin, and cynarin. With the exception of chlorogenic acid, the other four compounds have been identified for the first time in <i>S. chamaecyparissus</i>. The findings have confirmed that MAE is a significantly more efficient extraction method than UAE to extract phenolic compounds from <i>S. chamaecyparissus.</i>https://www.mdpi.com/2073-4395/11/1/84cotton-lavender<i>Santolina chamaecyparissus</i> L.ultrasound-assisted extractionmicrowave-assisted extractionBox-Behnken designphenolic compounds |
| spellingShingle | Mohammed Aourach Ana V. González-de-Peredo Mercedes Vázquez-Espinosa Haiat Essalmani Miguel Palma Gerardo F. Barbero Optimization and Comparison of Ultrasound and Microwave-Assisted Extraction of Phenolic Compounds from Cotton-Lavender (<i>Santolina chamaecyparissus</i> L.) Agronomy cotton-lavender <i>Santolina chamaecyparissus</i> L. ultrasound-assisted extraction microwave-assisted extraction Box-Behnken design phenolic compounds |
| title | Optimization and Comparison of Ultrasound and Microwave-Assisted Extraction of Phenolic Compounds from Cotton-Lavender (<i>Santolina chamaecyparissus</i> L.) |
| title_full | Optimization and Comparison of Ultrasound and Microwave-Assisted Extraction of Phenolic Compounds from Cotton-Lavender (<i>Santolina chamaecyparissus</i> L.) |
| title_fullStr | Optimization and Comparison of Ultrasound and Microwave-Assisted Extraction of Phenolic Compounds from Cotton-Lavender (<i>Santolina chamaecyparissus</i> L.) |
| title_full_unstemmed | Optimization and Comparison of Ultrasound and Microwave-Assisted Extraction of Phenolic Compounds from Cotton-Lavender (<i>Santolina chamaecyparissus</i> L.) |
| title_short | Optimization and Comparison of Ultrasound and Microwave-Assisted Extraction of Phenolic Compounds from Cotton-Lavender (<i>Santolina chamaecyparissus</i> L.) |
| title_sort | optimization and comparison of ultrasound and microwave assisted extraction of phenolic compounds from cotton lavender i santolina chamaecyparissus i l |
| topic | cotton-lavender <i>Santolina chamaecyparissus</i> L. ultrasound-assisted extraction microwave-assisted extraction Box-Behnken design phenolic compounds |
| url | https://www.mdpi.com/2073-4395/11/1/84 |
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