Assessment of Selected Morphological, Physical and Chemical Parameters of the Teeth of the Offspring of Female Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD), Taking into Account the Protective Role of Selected Antioxidants—Preliminary Study
The studies conducted so far indicate a negative effect of dioxins on the structure of the alveolar bone and teeth, especially in the developmental period in rats. The research aimed to analyze the indirect effect of dioxins contained in the body of female rats on the structure of the dental organ i...
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MDPI AG
2022-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/4/484 |
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| author | Maciej Dobrzyński Anna Nikodem Joanna Klećkowska-Nawrot Karolina Goździewska-Harłajczuk Maciej Janeczek Marzena Styczyńska Piotr Kuropka |
| author_facet | Maciej Dobrzyński Anna Nikodem Joanna Klećkowska-Nawrot Karolina Goździewska-Harłajczuk Maciej Janeczek Marzena Styczyńska Piotr Kuropka |
| author_sort | Maciej Dobrzyński |
| collection | DOAJ |
| description | The studies conducted so far indicate a negative effect of dioxins on the structure of the alveolar bone and teeth, especially in the developmental period in rats. The research aimed to analyze the indirect effect of dioxins contained in the body of female rats on the structure of the dental organ in their offspring in the neonatal period and to determine the possibility of reducing potential dioxin disorders of the structure of hard tissues in the offspring of intoxicated mothers by simultaneous administration of vitamin E or acetylsalicylic acid (ASA). Another goal of the research was to determine the level of magnesium, calcium and phosphorus contained in bone tissue as indicators of the mineralization process of hard tissues in rats, in the case of using 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) and acetylsalicylic acid or α-tocopherol. The experiment was carried out on eight female rats of the <i>Buffalo</i> strain divided into four groups. From the offspring of eight females, the mandibles were removed from the mandibular joints, and then, after the removal of soft tissues, they were prepared for individual tests. Selected morphological, chemical and physical parameters of the teeth of the offspring of female rats from the experimental groups were analyzed. The analysis showed the effect of vitamin E and ASA on the content of Mg, Ca and P. In combination with TCDD, vitamin E and ASA, they positively inhibit the inflammatory process, preventing the leaching of Ca and Mg from the bones. ASA counteracted this phenomenon much more effectively than vitamin E. Detailed analysis of the tooth morphology showed that the molars’ crowns exhibit shape disturbances under the influence of TCDD. Individual nodules in teeth T1, T2, T3 did not fuse, and the roots showed signs of hypertrophy. The study confirmed the negative effect of TCDD on tooth development. Teeth arising early in development are the most sensitive to the disorders, while the later ones are less exposed to the toxic effects of TCDD transmitted by the mother. |
| first_indexed | 2024-03-09T22:47:43Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:47:43Z |
| publishDate | 2022-02-01 |
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| spelling | doaj.art-bd5b92a0f85b4abcaf403de84a6225fe2023-11-23T18:25:50ZengMDPI AGAnimals2076-26152022-02-0112448410.3390/ani12040484Assessment of Selected Morphological, Physical and Chemical Parameters of the Teeth of the Offspring of Female Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD), Taking into Account the Protective Role of Selected Antioxidants—Preliminary StudyMaciej Dobrzyński0Anna Nikodem1Joanna Klećkowska-Nawrot2Karolina Goździewska-Harłajczuk3Maciej Janeczek4Marzena Styczyńska5Piotr Kuropka6Department of Pediatric Dentistry and Preclinical Dentistry, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, PolandDepartment of Mechanics, Materials and Biomedical Engineering, Faculty of Mechanical Engineering, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, PolandDepartment of Biostructure and Animal Physiology, Division of Animal Anatomy, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Kożuchowska 1, 51-631 Wroclaw, PolandDepartment of Biostructure and Animal Physiology, Division of Animal Anatomy, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Kożuchowska 1, 51-631 Wroclaw, PolandDepartment of Biostructure and Animal Physiology, Division of Animal Anatomy, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Kożuchowska 1, 51-631 Wroclaw, PolandFaculty of Biotechnology and Food Sciences, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wroclaw, PolandDepartment of Biostructure and Animal Physiology, Division of Histology and Embryology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-635 Wroclaw, PolandThe studies conducted so far indicate a negative effect of dioxins on the structure of the alveolar bone and teeth, especially in the developmental period in rats. The research aimed to analyze the indirect effect of dioxins contained in the body of female rats on the structure of the dental organ in their offspring in the neonatal period and to determine the possibility of reducing potential dioxin disorders of the structure of hard tissues in the offspring of intoxicated mothers by simultaneous administration of vitamin E or acetylsalicylic acid (ASA). Another goal of the research was to determine the level of magnesium, calcium and phosphorus contained in bone tissue as indicators of the mineralization process of hard tissues in rats, in the case of using 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) and acetylsalicylic acid or α-tocopherol. The experiment was carried out on eight female rats of the <i>Buffalo</i> strain divided into four groups. From the offspring of eight females, the mandibles were removed from the mandibular joints, and then, after the removal of soft tissues, they were prepared for individual tests. Selected morphological, chemical and physical parameters of the teeth of the offspring of female rats from the experimental groups were analyzed. The analysis showed the effect of vitamin E and ASA on the content of Mg, Ca and P. In combination with TCDD, vitamin E and ASA, they positively inhibit the inflammatory process, preventing the leaching of Ca and Mg from the bones. ASA counteracted this phenomenon much more effectively than vitamin E. Detailed analysis of the tooth morphology showed that the molars’ crowns exhibit shape disturbances under the influence of TCDD. Individual nodules in teeth T1, T2, T3 did not fuse, and the roots showed signs of hypertrophy. The study confirmed the negative effect of TCDD on tooth development. Teeth arising early in development are the most sensitive to the disorders, while the later ones are less exposed to the toxic effects of TCDD transmitted by the mother.https://www.mdpi.com/2076-2615/12/4/484dental structure2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD)AhR receptor<i>Buffalo</i> rathistologyα-tocopherol |
| spellingShingle | Maciej Dobrzyński Anna Nikodem Joanna Klećkowska-Nawrot Karolina Goździewska-Harłajczuk Maciej Janeczek Marzena Styczyńska Piotr Kuropka Assessment of Selected Morphological, Physical and Chemical Parameters of the Teeth of the Offspring of Female Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD), Taking into Account the Protective Role of Selected Antioxidants—Preliminary Study Animals dental structure 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) AhR receptor <i>Buffalo</i> rat histology α-tocopherol |
| title | Assessment of Selected Morphological, Physical and Chemical Parameters of the Teeth of the Offspring of Female Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD), Taking into Account the Protective Role of Selected Antioxidants—Preliminary Study |
| title_full | Assessment of Selected Morphological, Physical and Chemical Parameters of the Teeth of the Offspring of Female Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD), Taking into Account the Protective Role of Selected Antioxidants—Preliminary Study |
| title_fullStr | Assessment of Selected Morphological, Physical and Chemical Parameters of the Teeth of the Offspring of Female Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD), Taking into Account the Protective Role of Selected Antioxidants—Preliminary Study |
| title_full_unstemmed | Assessment of Selected Morphological, Physical and Chemical Parameters of the Teeth of the Offspring of Female Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD), Taking into Account the Protective Role of Selected Antioxidants—Preliminary Study |
| title_short | Assessment of Selected Morphological, Physical and Chemical Parameters of the Teeth of the Offspring of Female Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD), Taking into Account the Protective Role of Selected Antioxidants—Preliminary Study |
| title_sort | assessment of selected morphological physical and chemical parameters of the teeth of the offspring of female rats exposed to 2 3 7 8 tetrachlorodibenzo i p i dioxin tcdd taking into account the protective role of selected antioxidants preliminary study |
| topic | dental structure 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) AhR receptor <i>Buffalo</i> rat histology α-tocopherol |
| url | https://www.mdpi.com/2076-2615/12/4/484 |
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