Genome-wide identification and characteristic analysis of ETS gene family in blood clam Tegillarca granosa
Abstract Background ETS transcription factors, known as the E26 transformation-specific factors, assume a critical role in the regulation of various vital biological processes in animals, including cell differentiation, the cell cycle, and cell apoptosis. However, their characterization in mollusks...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09731-5 |
| _version_ | 1827635499839258624 |
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| author | Hongyu Jin Weiwei Zhang Hongxing Liu Yongbo Bao |
| author_facet | Hongyu Jin Weiwei Zhang Hongxing Liu Yongbo Bao |
| author_sort | Hongyu Jin |
| collection | DOAJ |
| description | Abstract Background ETS transcription factors, known as the E26 transformation-specific factors, assume a critical role in the regulation of various vital biological processes in animals, including cell differentiation, the cell cycle, and cell apoptosis. However, their characterization in mollusks is currently lacking. Results The current study focused on a comprehensive analysis of the ETS genes in blood clam Tegillarca granosa and other mollusk genomes. Our phylogenetic analysis revealed the absence of the SPI and ETV subfamilies in mollusks compared to humans. Additionally, several ETS genes in mollusks were found to lack the PNT domain, potentially resulting in a diminished ability of ETS proteins to bind target genes. Interestingly, the bivalve ETS1 genes exhibited significantly high expression levels during the multicellular proliferation stage and in gill tissues. Furthermore, qRT-PCR results showed that Tg-ETS-14 (ETS1) is upregulated in the high total hemocyte counts (THC) population of T. granosa, suggesting it plays a significant role in stimulating hemocyte proliferation. Conclusion Our study significantly contributes to the comprehension of the evolutionary aspects concerning the ETS gene family, while also providing valuable insights into its role in fostering hemocyte proliferation across mollusks. |
| first_indexed | 2024-03-09T15:27:35Z |
| format | Article |
| id | doaj.art-bd60a71ee6a6483aa45b92cf5d16e733 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-09T15:27:35Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-bd60a71ee6a6483aa45b92cf5d16e7332023-11-26T12:25:53ZengBMCBMC Genomics1471-21642023-11-0124111110.1186/s12864-023-09731-5Genome-wide identification and characteristic analysis of ETS gene family in blood clam Tegillarca granosaHongyu Jin0Weiwei Zhang1Hongxing Liu2Yongbo Bao3School of Marine Sciences, Ningbo UniversitySchool of Marine Sciences, Ningbo UniversityZhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological & Environmental Sciences, Zhejiang Wanli UniversityZhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological & Environmental Sciences, Zhejiang Wanli UniversityAbstract Background ETS transcription factors, known as the E26 transformation-specific factors, assume a critical role in the regulation of various vital biological processes in animals, including cell differentiation, the cell cycle, and cell apoptosis. However, their characterization in mollusks is currently lacking. Results The current study focused on a comprehensive analysis of the ETS genes in blood clam Tegillarca granosa and other mollusk genomes. Our phylogenetic analysis revealed the absence of the SPI and ETV subfamilies in mollusks compared to humans. Additionally, several ETS genes in mollusks were found to lack the PNT domain, potentially resulting in a diminished ability of ETS proteins to bind target genes. Interestingly, the bivalve ETS1 genes exhibited significantly high expression levels during the multicellular proliferation stage and in gill tissues. Furthermore, qRT-PCR results showed that Tg-ETS-14 (ETS1) is upregulated in the high total hemocyte counts (THC) population of T. granosa, suggesting it plays a significant role in stimulating hemocyte proliferation. Conclusion Our study significantly contributes to the comprehension of the evolutionary aspects concerning the ETS gene family, while also providing valuable insights into its role in fostering hemocyte proliferation across mollusks.https://doi.org/10.1186/s12864-023-09731-5ETSMollusksPhylogenetic analysisExpression profilingTotal hemocyte counts |
| spellingShingle | Hongyu Jin Weiwei Zhang Hongxing Liu Yongbo Bao Genome-wide identification and characteristic analysis of ETS gene family in blood clam Tegillarca granosa BMC Genomics ETS Mollusks Phylogenetic analysis Expression profiling Total hemocyte counts |
| title | Genome-wide identification and characteristic analysis of ETS gene family in blood clam Tegillarca granosa |
| title_full | Genome-wide identification and characteristic analysis of ETS gene family in blood clam Tegillarca granosa |
| title_fullStr | Genome-wide identification and characteristic analysis of ETS gene family in blood clam Tegillarca granosa |
| title_full_unstemmed | Genome-wide identification and characteristic analysis of ETS gene family in blood clam Tegillarca granosa |
| title_short | Genome-wide identification and characteristic analysis of ETS gene family in blood clam Tegillarca granosa |
| title_sort | genome wide identification and characteristic analysis of ets gene family in blood clam tegillarca granosa |
| topic | ETS Mollusks Phylogenetic analysis Expression profiling Total hemocyte counts |
| url | https://doi.org/10.1186/s12864-023-09731-5 |
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