Enhanced Viability of Probiotics against Gastric Acid by One-Step Coating Process with Poly-L-Lysine: In Vitro and In Vivo Evaluation
Due to their low acid tolerance, a majority of probiotics face diculties with regard to<br />surviving in the gastric environment long enough to reach the intestinal surfaces where they colonize<br />and provide health benefits. We prepared a probiotic delivery system that can enhance th...
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MDPI AG
2020-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/12/7/662 |
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| author | Shwe Phyu Hlaing Jihyun Kim Juho Lee Dongmin Kwak Hyunwoo Kim Jin-Wook Yoo |
| author_facet | Shwe Phyu Hlaing Jihyun Kim Juho Lee Dongmin Kwak Hyunwoo Kim Jin-Wook Yoo |
| author_sort | Shwe Phyu Hlaing |
| collection | DOAJ |
| description | Due to their low acid tolerance, a majority of probiotics face diculties with regard to<br />surviving in the gastric environment long enough to reach the intestinal surfaces where they colonize<br />and provide health benefits. We prepared a probiotic delivery system that can enhance their viability<br />in acidic conditions by developing a one-step poly-L-lysine (PLL) coating process. We determined<br />whether the coating process was successful by measuring the zeta potential and observing it with<br />confocal scanning microscopy. PLL-coated L. plantarum (PLL-LP), incubated in a solution of pH 2 for<br />2 h, exhibited a higher viability (6.86 0.12 log CFU/mL of viable cells) than non-coated L. plantarum<br />(non-coated LP), which exhibited only 2.7 1.23 log CFU/mL of viable cells. In addition, a higher<br />amount of L. plantarum was detected in the feces of mice orally administered PLL-LP (6.2 0.4 log<br />CFU/g of feces) than in the feces of the control groups. In addition to enhancing probiotic viability in<br />pH 2 solution, the PLL coating showed no eect on the probiotic growth pattern and the viability of<br />either freeze-dried L. plantarum or L. plantarum, stored at ?20 C and 4 C, respectively. Overall, these<br />results indicated that the PLL coating is a promising potential probiotic delivery system. |
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| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T18:29:56Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-bd633e4caac746d8ae28cc1a502672cd2023-11-20T06:45:30ZengMDPI AGPharmaceutics1999-49232020-07-0112766210.3390/pharmaceutics12070662Enhanced Viability of Probiotics against Gastric Acid by One-Step Coating Process with Poly-L-Lysine: In Vitro and In Vivo EvaluationShwe Phyu Hlaing0Jihyun Kim1Juho Lee2Dongmin Kwak3Hyunwoo Kim4Jin-Wook Yoo5College of Pharmacy, Pusan National University, Busan 46241, KoreaCollege of Pharmacy, Pusan National University, Busan 46241, KoreaCollege of Pharmacy, Pusan National University, Busan 46241, KoreaCollege of Pharmacy, Pusan National University, Busan 46241, KoreaCollege of Pharmacy, Pusan National University, Busan 46241, KoreaCollege of Pharmacy, Pusan National University, Busan 46241, KoreaDue to their low acid tolerance, a majority of probiotics face diculties with regard to<br />surviving in the gastric environment long enough to reach the intestinal surfaces where they colonize<br />and provide health benefits. We prepared a probiotic delivery system that can enhance their viability<br />in acidic conditions by developing a one-step poly-L-lysine (PLL) coating process. We determined<br />whether the coating process was successful by measuring the zeta potential and observing it with<br />confocal scanning microscopy. PLL-coated L. plantarum (PLL-LP), incubated in a solution of pH 2 for<br />2 h, exhibited a higher viability (6.86 0.12 log CFU/mL of viable cells) than non-coated L. plantarum<br />(non-coated LP), which exhibited only 2.7 1.23 log CFU/mL of viable cells. In addition, a higher<br />amount of L. plantarum was detected in the feces of mice orally administered PLL-LP (6.2 0.4 log<br />CFU/g of feces) than in the feces of the control groups. In addition to enhancing probiotic viability in<br />pH 2 solution, the PLL coating showed no eect on the probiotic growth pattern and the viability of<br />either freeze-dried L. plantarum or L. plantarum, stored at ?20 C and 4 C, respectively. Overall, these<br />results indicated that the PLL coating is a promising potential probiotic delivery system.https://www.mdpi.com/1999-4923/12/7/662<i>Lactobacillus plantarum</i>poly-L-lysineprobioticsacid resistanceviability |
| spellingShingle | Shwe Phyu Hlaing Jihyun Kim Juho Lee Dongmin Kwak Hyunwoo Kim Jin-Wook Yoo Enhanced Viability of Probiotics against Gastric Acid by One-Step Coating Process with Poly-L-Lysine: In Vitro and In Vivo Evaluation Pharmaceutics <i>Lactobacillus plantarum</i> poly-L-lysine probiotics acid resistance viability |
| title | Enhanced Viability of Probiotics against Gastric Acid by One-Step Coating Process with Poly-L-Lysine: In Vitro and In Vivo Evaluation |
| title_full | Enhanced Viability of Probiotics against Gastric Acid by One-Step Coating Process with Poly-L-Lysine: In Vitro and In Vivo Evaluation |
| title_fullStr | Enhanced Viability of Probiotics against Gastric Acid by One-Step Coating Process with Poly-L-Lysine: In Vitro and In Vivo Evaluation |
| title_full_unstemmed | Enhanced Viability of Probiotics against Gastric Acid by One-Step Coating Process with Poly-L-Lysine: In Vitro and In Vivo Evaluation |
| title_short | Enhanced Viability of Probiotics against Gastric Acid by One-Step Coating Process with Poly-L-Lysine: In Vitro and In Vivo Evaluation |
| title_sort | enhanced viability of probiotics against gastric acid by one step coating process with poly l lysine in vitro and in vivo evaluation |
| topic | <i>Lactobacillus plantarum</i> poly-L-lysine probiotics acid resistance viability |
| url | https://www.mdpi.com/1999-4923/12/7/662 |
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