Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms
Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP...
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8339 |
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| author | Georgia Levidou Konstantinos Palamaris Alexandros G. Sykaras Georgios Andreadakis Christos Masaoutis Irene Theochari Penelope Korkolopoulou Dimitra Rontogianni Stamatios Theocharis |
| author_facet | Georgia Levidou Konstantinos Palamaris Alexandros G. Sykaras Georgios Andreadakis Christos Masaoutis Irene Theochari Penelope Korkolopoulou Dimitra Rontogianni Stamatios Theocharis |
| author_sort | Georgia Levidou |
| collection | DOAJ |
| description | Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i>p</i> = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i>p</i> = 0.007 and <i>p</i> = 0.002) and higher cytoplasmic HJURP H-score (<i>p</i> < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i>p</i> = 0.048 and <i>p</i> < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i>p</i> = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:35:14Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bd6356ee7ab84267b42d9a16ddef88dd2023-11-30T22:25:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012315833910.3390/ijms23158339Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial NeoplasmsGeorgia Levidou0Konstantinos Palamaris1Alexandros G. Sykaras2Georgios Andreadakis3Christos Masaoutis4Irene Theochari5Penelope Korkolopoulou6Dimitra Rontogianni7Stamatios Theocharis8First Department of Pathology, National and Kapodistrian University of Athens, 11527 Athens, GreeceFirst Department of Pathology, National and Kapodistrian University of Athens, 11527 Athens, GreeceFirst Department of Pathology, National and Kapodistrian University of Athens, 11527 Athens, GreeceFirst Department of Pathology, National and Kapodistrian University of Athens, 11527 Athens, GreeceFirst Department of Pathology, National and Kapodistrian University of Athens, 11527 Athens, GreeceFirst Department of Pathology, National and Kapodistrian University of Athens, 11527 Athens, GreeceFirst Department of Pathology, National and Kapodistrian University of Athens, 11527 Athens, GreeceFirst Department of Pathology, National and Kapodistrian University of Athens, 11527 Athens, GreeceFirst Department of Pathology, National and Kapodistrian University of Athens, 11527 Athens, GreeceBackground: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i>p</i> = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i>p</i> = 0.007 and <i>p</i> = 0.002) and higher cytoplasmic HJURP H-score (<i>p</i> < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i>p</i> = 0.048 and <i>p</i> < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i>p</i> = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern.https://www.mdpi.com/1422-0067/23/15/8339thymic epithelial neoplasmsthymomashistonesCENP-AHJURPDAXX |
| spellingShingle | Georgia Levidou Konstantinos Palamaris Alexandros G. Sykaras Georgios Andreadakis Christos Masaoutis Irene Theochari Penelope Korkolopoulou Dimitra Rontogianni Stamatios Theocharis Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms International Journal of Molecular Sciences thymic epithelial neoplasms thymomas histones CENP-A HJURP DAXX |
| title | Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms |
| title_full | Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms |
| title_fullStr | Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms |
| title_full_unstemmed | Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms |
| title_short | Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms |
| title_sort | unraveling the role of histone variant cenp a and chaperone hjurp expression in thymic epithelial neoplasms |
| topic | thymic epithelial neoplasms thymomas histones CENP-A HJURP DAXX |
| url | https://www.mdpi.com/1422-0067/23/15/8339 |
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