<it>TaMSH7</it>: A cereal mismatch repair gene that affects fertility in transgenic barley (<it>Hordeum vulgare </it>L.)
<p>Abstract</p> <p>Background</p> <p>Chromosome pairing, recombination and DNA repair are essential processes during meiosis in sexually reproducing organisms. Investigating the bread wheat (<it>Triticum aestivum </it>L.) <it>Ph2 </it>(<it>...
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| Format: | Article |
| Language: | English |
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BMC
2007-12-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/7/67 |
| _version_ | 1818833723689271296 |
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| author | Langridge Peter Milligan Andrew S Lloyd Andrew H Able Jason A |
| author_facet | Langridge Peter Milligan Andrew S Lloyd Andrew H Able Jason A |
| author_sort | Langridge Peter |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Chromosome pairing, recombination and DNA repair are essential processes during meiosis in sexually reproducing organisms. Investigating the bread wheat (<it>Triticum aestivum </it>L.) <it>Ph2 </it>(<it>P</it>airing <it>h</it>omoeologous) locus has identified numerous candidate genes that may have a role in controlling such processes, including <it>TaMSH7</it>, a plant specific member of the DNA mismatch repair family.</p> <p>Results</p> <p>Sequencing of the three <it>MSH7 </it>genes, located on the short arms of wheat chromosomes 3A, 3B and 3D, has revealed no significant sequence divergence at the amino acid level suggesting conservation of function across the homoeogroups. Functional analysis of <it>MSH7 </it>through the use of RNAi loss-of-function transgenics was undertaken in diploid barley (<it>Hordeum vulgare </it>L.). Quantitative real-time PCR revealed several T<sub>0 </sub>lines with reduced <it>MSH7 </it>expression. Positive segregants from two T<sub>1 </sub>lines studied in detail showed reduced <it>MSH7 </it>expression when compared to transformed controls and null segregants. Expression of <it>MSH6</it>, another member of the mismatch repair family which is most closely related to the <it>MSH7 </it>gene, was not significantly reduced in these lines. In both T<sub>1 </sub>lines, reduced seed set in positive segregants was observed.</p> <p>Conclusion</p> <p>Results presented here indicate, for the first time, a distinct functional role for <it>MSH7 in vivo </it>and show that expression of this gene is necessary for wild-type levels of fertility. These observations suggest that <it>MSH7 </it>has an important function during meiosis and as such remains a candidate for <it>Ph2</it>.</p> |
| first_indexed | 2024-12-19T02:23:28Z |
| format | Article |
| id | doaj.art-bd6793a989c846bcb6cd6fcdfbcfa099 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-19T02:23:28Z |
| publishDate | 2007-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-bd6793a989c846bcb6cd6fcdfbcfa0992022-12-21T20:40:03ZengBMCBMC Plant Biology1471-22292007-12-01716710.1186/1471-2229-7-67<it>TaMSH7</it>: A cereal mismatch repair gene that affects fertility in transgenic barley (<it>Hordeum vulgare </it>L.)Langridge PeterMilligan Andrew SLloyd Andrew HAble Jason A<p>Abstract</p> <p>Background</p> <p>Chromosome pairing, recombination and DNA repair are essential processes during meiosis in sexually reproducing organisms. Investigating the bread wheat (<it>Triticum aestivum </it>L.) <it>Ph2 </it>(<it>P</it>airing <it>h</it>omoeologous) locus has identified numerous candidate genes that may have a role in controlling such processes, including <it>TaMSH7</it>, a plant specific member of the DNA mismatch repair family.</p> <p>Results</p> <p>Sequencing of the three <it>MSH7 </it>genes, located on the short arms of wheat chromosomes 3A, 3B and 3D, has revealed no significant sequence divergence at the amino acid level suggesting conservation of function across the homoeogroups. Functional analysis of <it>MSH7 </it>through the use of RNAi loss-of-function transgenics was undertaken in diploid barley (<it>Hordeum vulgare </it>L.). Quantitative real-time PCR revealed several T<sub>0 </sub>lines with reduced <it>MSH7 </it>expression. Positive segregants from two T<sub>1 </sub>lines studied in detail showed reduced <it>MSH7 </it>expression when compared to transformed controls and null segregants. Expression of <it>MSH6</it>, another member of the mismatch repair family which is most closely related to the <it>MSH7 </it>gene, was not significantly reduced in these lines. In both T<sub>1 </sub>lines, reduced seed set in positive segregants was observed.</p> <p>Conclusion</p> <p>Results presented here indicate, for the first time, a distinct functional role for <it>MSH7 in vivo </it>and show that expression of this gene is necessary for wild-type levels of fertility. These observations suggest that <it>MSH7 </it>has an important function during meiosis and as such remains a candidate for <it>Ph2</it>.</p>http://www.biomedcentral.com/1471-2229/7/67 |
| spellingShingle | Langridge Peter Milligan Andrew S Lloyd Andrew H Able Jason A <it>TaMSH7</it>: A cereal mismatch repair gene that affects fertility in transgenic barley (<it>Hordeum vulgare </it>L.) BMC Plant Biology |
| title | <it>TaMSH7</it>: A cereal mismatch repair gene that affects fertility in transgenic barley (<it>Hordeum vulgare </it>L.) |
| title_full | <it>TaMSH7</it>: A cereal mismatch repair gene that affects fertility in transgenic barley (<it>Hordeum vulgare </it>L.) |
| title_fullStr | <it>TaMSH7</it>: A cereal mismatch repair gene that affects fertility in transgenic barley (<it>Hordeum vulgare </it>L.) |
| title_full_unstemmed | <it>TaMSH7</it>: A cereal mismatch repair gene that affects fertility in transgenic barley (<it>Hordeum vulgare </it>L.) |
| title_short | <it>TaMSH7</it>: A cereal mismatch repair gene that affects fertility in transgenic barley (<it>Hordeum vulgare </it>L.) |
| title_sort | it tamsh7 it a cereal mismatch repair gene that affects fertility in transgenic barley it hordeum vulgare it l |
| url | http://www.biomedcentral.com/1471-2229/7/67 |
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