Fermented Beetroot (Beta vulgaris L.) Extract by Kombucha as Energy Booster Produced via Cross-Flow Microfiltration Membrane

Beetroot extract (permeate) fermented by kombucha culture separated through cross-flow microfiltration (CFMF) with pore size 0.15 μm at TMP 2 and 6 bar for 0, 5, 15, 25, and 35 minutes, with a flow rate of 7.5 L/m3.hour, at room temperature potentially serves as a natural energy booster. The researc...

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Main Authors: Susilowati Agustine, Aspiyanto, Melanie Hakiki, Maryati Yati, Filailla Euis
Format: Article
Language:English
Published: EDP Sciences 2024-01-01
Series:E3S Web of Conferences
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/33/e3sconf_isac-iccme2023_05006.pdf
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author Susilowati Agustine
Aspiyanto
Melanie Hakiki
Maryati Yati
Filailla Euis
author_facet Susilowati Agustine
Aspiyanto
Melanie Hakiki
Maryati Yati
Filailla Euis
author_sort Susilowati Agustine
collection DOAJ
description Beetroot extract (permeate) fermented by kombucha culture separated through cross-flow microfiltration (CFMF) with pore size 0.15 μm at TMP 2 and 6 bar for 0, 5, 15, 25, and 35 minutes, with a flow rate of 7.5 L/m3.hour, at room temperature potentially serves as a natural energy booster. The research results based on soluble protein show that the optimization process at TMP 2 and 6 bar was achieved at 15 minutes with permeate flux of 32.52 and 28.05 L/m3.h, respectively, and soluble protein content of 0.72 and 0.38 mg/mL, total solids of 3.40% and 3.36%, total sugar of 45.91 and 47.47 mg/mL, and acetic acid of 0.93 and 0.97%. Under these conditions, the CFMF system increased the soluble protein in permeate by 123.53% or 1.23 times, indicating partial rejection, and 22.58% or complete rejection. The identification of amino acids in the optimum condition was dominated by three threonine monomers with molecular weights of 120.11, 120.37, and 120.90 Da (M+), and six tryptophan monomers with molecular weights of 205.11, 205.21, 205.44, 205.60, 205.88, and 205.94 Da (M+). The permeate under optimum conditions had average particle sizes of 2788.0 nm and 2922.0 nm, with particle index of 1.523 and 1.795. The particle distribution in permeate at TMP 2 and 6 bar was less than 10,000 nm in size
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spelling doaj.art-bd6847401cbd47dbae285bacfe0f60a72024-03-22T07:56:07ZengEDP SciencesE3S Web of Conferences2267-12422024-01-015030500610.1051/e3sconf/202450305006e3sconf_isac-iccme2023_05006Fermented Beetroot (Beta vulgaris L.) Extract by Kombucha as Energy Booster Produced via Cross-Flow Microfiltration MembraneSusilowati Agustine0Aspiyanto1Melanie Hakiki2Maryati Yati3Filailla Euis4Research Centre for Chemistry, National Research and Innovation AgencyResearch Centre for Chemistry, National Research and Innovation AgencyResearch Centre for Chemistry, National Research and Innovation AgencyResearch Centre for Chemistry, National Research and Innovation AgencyResearch Centre for Chemistry, National Research and Innovation AgencyBeetroot extract (permeate) fermented by kombucha culture separated through cross-flow microfiltration (CFMF) with pore size 0.15 μm at TMP 2 and 6 bar for 0, 5, 15, 25, and 35 minutes, with a flow rate of 7.5 L/m3.hour, at room temperature potentially serves as a natural energy booster. The research results based on soluble protein show that the optimization process at TMP 2 and 6 bar was achieved at 15 minutes with permeate flux of 32.52 and 28.05 L/m3.h, respectively, and soluble protein content of 0.72 and 0.38 mg/mL, total solids of 3.40% and 3.36%, total sugar of 45.91 and 47.47 mg/mL, and acetic acid of 0.93 and 0.97%. Under these conditions, the CFMF system increased the soluble protein in permeate by 123.53% or 1.23 times, indicating partial rejection, and 22.58% or complete rejection. The identification of amino acids in the optimum condition was dominated by three threonine monomers with molecular weights of 120.11, 120.37, and 120.90 Da (M+), and six tryptophan monomers with molecular weights of 205.11, 205.21, 205.44, 205.60, 205.88, and 205.94 Da (M+). The permeate under optimum conditions had average particle sizes of 2788.0 nm and 2922.0 nm, with particle index of 1.523 and 1.795. The particle distribution in permeate at TMP 2 and 6 bar was less than 10,000 nm in sizehttps://www.e3s-conferences.org/articles/e3sconf/pdf/2024/33/e3sconf_isac-iccme2023_05006.pdf
spellingShingle Susilowati Agustine
Aspiyanto
Melanie Hakiki
Maryati Yati
Filailla Euis
Fermented Beetroot (Beta vulgaris L.) Extract by Kombucha as Energy Booster Produced via Cross-Flow Microfiltration Membrane
E3S Web of Conferences
title Fermented Beetroot (Beta vulgaris L.) Extract by Kombucha as Energy Booster Produced via Cross-Flow Microfiltration Membrane
title_full Fermented Beetroot (Beta vulgaris L.) Extract by Kombucha as Energy Booster Produced via Cross-Flow Microfiltration Membrane
title_fullStr Fermented Beetroot (Beta vulgaris L.) Extract by Kombucha as Energy Booster Produced via Cross-Flow Microfiltration Membrane
title_full_unstemmed Fermented Beetroot (Beta vulgaris L.) Extract by Kombucha as Energy Booster Produced via Cross-Flow Microfiltration Membrane
title_short Fermented Beetroot (Beta vulgaris L.) Extract by Kombucha as Energy Booster Produced via Cross-Flow Microfiltration Membrane
title_sort fermented beetroot beta vulgaris l extract by kombucha as energy booster produced via cross flow microfiltration membrane
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/33/e3sconf_isac-iccme2023_05006.pdf
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