A conserved karyotype? Chromosomal rearrangements in Charadrius collaris detected by BAC-FISH.
Charadriidae comprise 142 valid species and the most recent checklist for the occurrence of this family in Brazil describes 11 species. There are few chromosomal studies in Charadriidae, most of them using a conventional approach. In Charadrius, only five species had their karyotypes described by cl...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0280164 |
| _version_ | 1797866051868819456 |
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| author | Paulo Victor de Moraes Ferreira Talita Fernanda Augusto Ribas Darren K Griffin Luyann André Rodrigues Correa Melquizedec Luiz Silva Pinheiro Lucas G Kiazim Rebecca E O'Connor Cleusa Yoshiko Nagamachi Julio Cesar Pieczarka |
| author_facet | Paulo Victor de Moraes Ferreira Talita Fernanda Augusto Ribas Darren K Griffin Luyann André Rodrigues Correa Melquizedec Luiz Silva Pinheiro Lucas G Kiazim Rebecca E O'Connor Cleusa Yoshiko Nagamachi Julio Cesar Pieczarka |
| author_sort | Paulo Victor de Moraes Ferreira |
| collection | DOAJ |
| description | Charadriidae comprise 142 valid species and the most recent checklist for the occurrence of this family in Brazil describes 11 species. There are few chromosomal studies in Charadriidae, most of them using a conventional approach. In Charadrius, only five species had their karyotypes described by classical cytogenetics, of which four have 2n = 76 (C. hiaticula, C. dubius, C. vociferou and C. collaris) and one 2n = 78 (C. alexandrinus alexandrinus). Among these species, only Charadrius collaris had the karyotype studied by chromosome painting, which allowed the identification of chromosomal homeologies with the karyotypes of Gallus gallus (GGA) and Burhinus oedicnemus (BOE). According to the literature, studies performed with BAC-FISH using probes from Gallus gallus and Taeniopygia guttata (TGU) libraries have shown interactions between macro and microchromosomes and micro inversions in chromosomes previously considered conserved. Other studies have shown the fusion of several microchromosomes, forming new macrochromosomes, leading to a decrease in the 2n of some species. The present study aims to deepen the chromosomal information in Charadrius collaris through the application of BAC-FISH with probes from the GGA and TGU libraries, in order to investigate possible rearrangements within the apparently conserved karyotype of this species, and thus better clarify the evolutionary history of the species. Charadrius collaris presented 2n = 76 and fundamental number (FN) equal to 94. Comparative mapping of BAC probes from GGA and TGU in Charadrius collaris revealed hybridization signals from 26 macrochromosome probes. Probes from microchromosomes 9 to 28 of GGA were also used and revealed 31 hybridization signals. The karyotype is well conserved, but it contains a paracentric and a pericentric inversion on the CCO1 chromosome, a paracentric and a pericentric inversion on the CCO4 and the separation of GGA4 into CCO4 and CCO8, demonstrating that the BAC-FISH approach allows for greater data resolution. More studies are needed to improve the understanding of chromosomal evolution within the order Charadriiformes and thus clarify whether these characteristics demonstrated here are specific traits for Charadrius collaris or if other species share these characteristics. |
| first_indexed | 2024-04-09T23:17:54Z |
| format | Article |
| id | doaj.art-bd6b7f630bc24123a134f7d5f9934b5d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T23:17:54Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-bd6b7f630bc24123a134f7d5f9934b5d2023-03-22T05:31:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01181e028016410.1371/journal.pone.0280164A conserved karyotype? Chromosomal rearrangements in Charadrius collaris detected by BAC-FISH.Paulo Victor de Moraes FerreiraTalita Fernanda Augusto RibasDarren K GriffinLuyann André Rodrigues CorreaMelquizedec Luiz Silva PinheiroLucas G KiazimRebecca E O'ConnorCleusa Yoshiko NagamachiJulio Cesar PieczarkaCharadriidae comprise 142 valid species and the most recent checklist for the occurrence of this family in Brazil describes 11 species. There are few chromosomal studies in Charadriidae, most of them using a conventional approach. In Charadrius, only five species had their karyotypes described by classical cytogenetics, of which four have 2n = 76 (C. hiaticula, C. dubius, C. vociferou and C. collaris) and one 2n = 78 (C. alexandrinus alexandrinus). Among these species, only Charadrius collaris had the karyotype studied by chromosome painting, which allowed the identification of chromosomal homeologies with the karyotypes of Gallus gallus (GGA) and Burhinus oedicnemus (BOE). According to the literature, studies performed with BAC-FISH using probes from Gallus gallus and Taeniopygia guttata (TGU) libraries have shown interactions between macro and microchromosomes and micro inversions in chromosomes previously considered conserved. Other studies have shown the fusion of several microchromosomes, forming new macrochromosomes, leading to a decrease in the 2n of some species. The present study aims to deepen the chromosomal information in Charadrius collaris through the application of BAC-FISH with probes from the GGA and TGU libraries, in order to investigate possible rearrangements within the apparently conserved karyotype of this species, and thus better clarify the evolutionary history of the species. Charadrius collaris presented 2n = 76 and fundamental number (FN) equal to 94. Comparative mapping of BAC probes from GGA and TGU in Charadrius collaris revealed hybridization signals from 26 macrochromosome probes. Probes from microchromosomes 9 to 28 of GGA were also used and revealed 31 hybridization signals. The karyotype is well conserved, but it contains a paracentric and a pericentric inversion on the CCO1 chromosome, a paracentric and a pericentric inversion on the CCO4 and the separation of GGA4 into CCO4 and CCO8, demonstrating that the BAC-FISH approach allows for greater data resolution. More studies are needed to improve the understanding of chromosomal evolution within the order Charadriiformes and thus clarify whether these characteristics demonstrated here are specific traits for Charadrius collaris or if other species share these characteristics.https://doi.org/10.1371/journal.pone.0280164 |
| spellingShingle | Paulo Victor de Moraes Ferreira Talita Fernanda Augusto Ribas Darren K Griffin Luyann André Rodrigues Correa Melquizedec Luiz Silva Pinheiro Lucas G Kiazim Rebecca E O'Connor Cleusa Yoshiko Nagamachi Julio Cesar Pieczarka A conserved karyotype? Chromosomal rearrangements in Charadrius collaris detected by BAC-FISH. PLoS ONE |
| title | A conserved karyotype? Chromosomal rearrangements in Charadrius collaris detected by BAC-FISH. |
| title_full | A conserved karyotype? Chromosomal rearrangements in Charadrius collaris detected by BAC-FISH. |
| title_fullStr | A conserved karyotype? Chromosomal rearrangements in Charadrius collaris detected by BAC-FISH. |
| title_full_unstemmed | A conserved karyotype? Chromosomal rearrangements in Charadrius collaris detected by BAC-FISH. |
| title_short | A conserved karyotype? Chromosomal rearrangements in Charadrius collaris detected by BAC-FISH. |
| title_sort | conserved karyotype chromosomal rearrangements in charadrius collaris detected by bac fish |
| url | https://doi.org/10.1371/journal.pone.0280164 |
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