Three-Dimensional Culture Systems for Dissecting Notch Signalling in Health and Disease
Three-dimensional (3D) culture systems opened up new horizons in studying the biology of tissues and organs, modelling various diseases, and screening drugs. Producing accurate in vitro models increases the possibilities for studying molecular control of cell–cell and cell–microenvironment interacti...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12473 |
| _version_ | 1797510015662161920 |
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| author | Guya Diletta Marconi Cristina Porcheri Oriana Trubiani Thimios A. Mitsiadis |
| author_facet | Guya Diletta Marconi Cristina Porcheri Oriana Trubiani Thimios A. Mitsiadis |
| author_sort | Guya Diletta Marconi |
| collection | DOAJ |
| description | Three-dimensional (3D) culture systems opened up new horizons in studying the biology of tissues and organs, modelling various diseases, and screening drugs. Producing accurate in vitro models increases the possibilities for studying molecular control of cell–cell and cell–microenvironment interactions in detail. The Notch signalling is linked to cell fate determination, tissue definition, and maintenance in both physiological and pathological conditions. Hence, 3D cultures provide new accessible platforms for studying activation and modulation of the Notch pathway. In this review, we provide an overview of the recent advances in different 3D culture systems, including spheroids, organoids, and “organ-on-a-chip” models, and their use in analysing the crucial role of Notch signalling in the maintenance of tissue homeostasis, pathology, and regeneration. |
| first_indexed | 2024-03-10T05:25:56Z |
| format | Article |
| id | doaj.art-bd6c8ed8282d4226a65816237f97f42b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T05:25:56Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bd6c8ed8282d4226a65816237f97f42b2023-11-22T23:42:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221247310.3390/ijms222212473Three-Dimensional Culture Systems for Dissecting Notch Signalling in Health and DiseaseGuya Diletta Marconi0Cristina Porcheri1Oriana Trubiani2Thimios A. Mitsiadis3Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, 8032 Zurich, SwitzerlandOrofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, 8032 Zurich, SwitzerlandDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyOrofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, 8032 Zurich, SwitzerlandThree-dimensional (3D) culture systems opened up new horizons in studying the biology of tissues and organs, modelling various diseases, and screening drugs. Producing accurate in vitro models increases the possibilities for studying molecular control of cell–cell and cell–microenvironment interactions in detail. The Notch signalling is linked to cell fate determination, tissue definition, and maintenance in both physiological and pathological conditions. Hence, 3D cultures provide new accessible platforms for studying activation and modulation of the Notch pathway. In this review, we provide an overview of the recent advances in different 3D culture systems, including spheroids, organoids, and “organ-on-a-chip” models, and their use in analysing the crucial role of Notch signalling in the maintenance of tissue homeostasis, pathology, and regeneration.https://www.mdpi.com/1422-0067/22/22/12473spheroidsorganoidsorgan-on-a-chipmicrofluidics3D culture systemsNotch signalling |
| spellingShingle | Guya Diletta Marconi Cristina Porcheri Oriana Trubiani Thimios A. Mitsiadis Three-Dimensional Culture Systems for Dissecting Notch Signalling in Health and Disease International Journal of Molecular Sciences spheroids organoids organ-on-a-chip microfluidics 3D culture systems Notch signalling |
| title | Three-Dimensional Culture Systems for Dissecting Notch Signalling in Health and Disease |
| title_full | Three-Dimensional Culture Systems for Dissecting Notch Signalling in Health and Disease |
| title_fullStr | Three-Dimensional Culture Systems for Dissecting Notch Signalling in Health and Disease |
| title_full_unstemmed | Three-Dimensional Culture Systems for Dissecting Notch Signalling in Health and Disease |
| title_short | Three-Dimensional Culture Systems for Dissecting Notch Signalling in Health and Disease |
| title_sort | three dimensional culture systems for dissecting notch signalling in health and disease |
| topic | spheroids organoids organ-on-a-chip microfluidics 3D culture systems Notch signalling |
| url | https://www.mdpi.com/1422-0067/22/22/12473 |
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