Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil
Pongamia pinnata (L.) seed oil is effective for its insecticidal and larvicidal activities. However, its low aqueous solubility, high photosensitivity, and high volatility restrict its application for the control of agricultural pests. Encapsulation can be an effective technique to overcome such hin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021006605 |
| _version_ | 1818927408014688256 |
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| author | Aloke Purkait Ayan Mukherjee Dipak Kumar Hazra Kusal Roy Pabitra Kumar Biswas Ramen Kumar Kole |
| author_facet | Aloke Purkait Ayan Mukherjee Dipak Kumar Hazra Kusal Roy Pabitra Kumar Biswas Ramen Kumar Kole |
| author_sort | Aloke Purkait |
| collection | DOAJ |
| description | Pongamia pinnata (L.) seed oil is effective for its insecticidal and larvicidal activities. However, its low aqueous solubility, high photosensitivity, and high volatility restrict its application for the control of agricultural pests. Encapsulation can be an effective technique to overcome such hindrances. Therefore, P. pinnata oil (PO) was extracted from its seeds and analyzed for karanjin content (3.18%) by GC-MS/MS as the marker compound. Micro-encapsulation (MC) of PO was prepared by interfacial polymerization between isocyanates and polyamine and tested for insecticidal and larvicidal activities. Bioassay of the developed formulations was tested in-vitro against 2nd instar larvae of Bombyx mori (Bivoltine hybrid) and in-vivo insecticidal bio-efficacy was tested against aubergine aphid (Aphis gossypii G.) and whitefly (Bemisia tabaci G.). Various properties of micro-capsules viz., stability, size, oil content and release kinetics were examined. Average diameter of capsules (1 μm) with Zeta potential (-16 mV) was indicated by the Dynamic Light Scattering (DLS) instrument. Existence of PO in the microcapsules was confirmed by an optical microscopic study. Spectroscopic analysis revealed 87.4% of PO was encapsulated in polyurea shell. The shelf-life (T10), half-life (T50), and expiry-life (T90) of polyurea coated capsules were 11.4, 75.3 and 250.0 h, respectively. Polyurea coated PO capsule formulation showed evidence of in-vitro toxicity against 2nd instar larvae of B. mori (LC50 = 1.1%; LC90 = 5.9%). The PO formulation also exhibited 67.0–71.8% and 62.4–74.8% control of aphid and whitefly population in aubergine at 4.0% dose following 7–14 days after application. The study unveiled its significance in developing controlled release herbal formulations of P. pinnata as an alternative to harmful conventional synthetic insecticides for crop protection. |
| first_indexed | 2024-12-20T03:12:32Z |
| format | Article |
| id | doaj.art-bd6ccd4fe8cc44fd9ef60d2152ab3aea |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-20T03:12:32Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-bd6ccd4fe8cc44fd9ef60d2152ab3aea2022-12-21T19:55:27ZengElsevierHeliyon2405-84402021-03-0173e06557Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oilAloke Purkait0Ayan Mukherjee1Dipak Kumar Hazra2Kusal Roy3Pabitra Kumar Biswas4Ramen Kumar Kole5Department of Soil Science and Agricultural Chemistry, Palli Siksha Bhavana (Institute of Agriculture), Visva - Bharati, Sriniketan, 731 236, Birbhum, West Bengal, IndiaDepartment of Soil Science and Agricultural Chemistry, Palli Siksha Bhavana (Institute of Agriculture), Visva - Bharati, Sriniketan, 731 236, Birbhum, West Bengal, IndiaDepartment of Agricultural Chemicals, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, 741 252, Nadia, West Bengal, IndiaDepartment of Agricultural Entomology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, 741 252, Nadia, West Bengal, IndiaDepartment of Soil Science and Agricultural Chemistry, Palli Siksha Bhavana (Institute of Agriculture), Visva - Bharati, Sriniketan, 731 236, Birbhum, West Bengal, IndiaDepartment of Agricultural Chemicals, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, 741 252, Nadia, West Bengal, India; Corresponding author.Pongamia pinnata (L.) seed oil is effective for its insecticidal and larvicidal activities. However, its low aqueous solubility, high photosensitivity, and high volatility restrict its application for the control of agricultural pests. Encapsulation can be an effective technique to overcome such hindrances. Therefore, P. pinnata oil (PO) was extracted from its seeds and analyzed for karanjin content (3.18%) by GC-MS/MS as the marker compound. Micro-encapsulation (MC) of PO was prepared by interfacial polymerization between isocyanates and polyamine and tested for insecticidal and larvicidal activities. Bioassay of the developed formulations was tested in-vitro against 2nd instar larvae of Bombyx mori (Bivoltine hybrid) and in-vivo insecticidal bio-efficacy was tested against aubergine aphid (Aphis gossypii G.) and whitefly (Bemisia tabaci G.). Various properties of micro-capsules viz., stability, size, oil content and release kinetics were examined. Average diameter of capsules (1 μm) with Zeta potential (-16 mV) was indicated by the Dynamic Light Scattering (DLS) instrument. Existence of PO in the microcapsules was confirmed by an optical microscopic study. Spectroscopic analysis revealed 87.4% of PO was encapsulated in polyurea shell. The shelf-life (T10), half-life (T50), and expiry-life (T90) of polyurea coated capsules were 11.4, 75.3 and 250.0 h, respectively. Polyurea coated PO capsule formulation showed evidence of in-vitro toxicity against 2nd instar larvae of B. mori (LC50 = 1.1%; LC90 = 5.9%). The PO formulation also exhibited 67.0–71.8% and 62.4–74.8% control of aphid and whitefly population in aubergine at 4.0% dose following 7–14 days after application. The study unveiled its significance in developing controlled release herbal formulations of P. pinnata as an alternative to harmful conventional synthetic insecticides for crop protection.http://www.sciencedirect.com/science/article/pii/S2405844021006605Pongamia seed oilInterfacial polymerizationMicro-encapsulationGC-MS/MS quantificationRelease kineticsInsecticidal bio-assay |
| spellingShingle | Aloke Purkait Ayan Mukherjee Dipak Kumar Hazra Kusal Roy Pabitra Kumar Biswas Ramen Kumar Kole Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil Heliyon Pongamia seed oil Interfacial polymerization Micro-encapsulation GC-MS/MS quantification Release kinetics Insecticidal bio-assay |
| title | Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
| title_full | Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
| title_fullStr | Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
| title_full_unstemmed | Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
| title_short | Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
| title_sort | encapsulation release and insecticidal activity of pongamia pinnata l seed oil |
| topic | Pongamia seed oil Interfacial polymerization Micro-encapsulation GC-MS/MS quantification Release kinetics Insecticidal bio-assay |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021006605 |
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