Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains
In 2013 alone, the death rate among the 9.0 million people infected with Mycobacterium tuberculosis (TB) worldwide was around 14%, which is unacceptably high. An empiric treatment of patients infected with TB or drug-resistant Mycobacterium tuberculosis (MDR-TB) strain can also result in the spread...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-03-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/16/3/376 |
| _version_ | 1798039736956223488 |
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| author | Keum-Soo Song Satish Balasaheb Nimse Hee Jin Kim Jeongseong Yang Taisun Kim |
| author_facet | Keum-Soo Song Satish Balasaheb Nimse Hee Jin Kim Jeongseong Yang Taisun Kim |
| author_sort | Keum-Soo Song |
| collection | DOAJ |
| description | In 2013 alone, the death rate among the 9.0 million people infected with Mycobacterium tuberculosis (TB) worldwide was around 14%, which is unacceptably high. An empiric treatment of patients infected with TB or drug-resistant Mycobacterium tuberculosis (MDR-TB) strain can also result in the spread of MDR-TB. The diagnostic tools which are rapid, reliable, and have simple experimental protocols can significantly help in decreasing the prevalence rate of MDR-TB strain. We report the evaluation of the 9G technology based 9G DNAChips that allow accurate detection and discrimination of TB and MDR-TB-RIF. One hundred and thirteen known cultured samples were used to evaluate the ability of 9G DNAChip in the detection and discrimination of TB and MDR-TB-RIF strains. Hybridization of immobilized probes with the PCR products of TB and MDR-TB-RIF strains allow their detection and discrimination. The accuracy of 9G DNAChip was determined by comparing its results with sequencing analysis and drug susceptibility testing. Sequencing analysis showed 100% agreement with the results of 9G DNAChip. The 9G DNAChip showed very high sensitivity (95.4%) and specificity (100%). |
| first_indexed | 2024-04-11T21:57:52Z |
| format | Article |
| id | doaj.art-bd70cf58d7e944cf993c33e276546d29 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T21:57:52Z |
| publishDate | 2016-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-bd70cf58d7e944cf993c33e276546d292022-12-22T04:01:03ZengMDPI AGSensors1424-82202016-03-0116337610.3390/s16030376s16030376Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis StrainsKeum-Soo Song0Satish Balasaheb Nimse1Hee Jin Kim2Jeongseong Yang3Taisun Kim4Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, KoreaInstitute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, KoreaKorea Mycobacterium Resource Center (KMRC), The Korean Institute of Tuberculosis, Cheongju-si 28158, KoreaKorea Mycobacterium Resource Center (KMRC), The Korean Institute of Tuberculosis, Cheongju-si 28158, KoreaInstitute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, KoreaIn 2013 alone, the death rate among the 9.0 million people infected with Mycobacterium tuberculosis (TB) worldwide was around 14%, which is unacceptably high. An empiric treatment of patients infected with TB or drug-resistant Mycobacterium tuberculosis (MDR-TB) strain can also result in the spread of MDR-TB. The diagnostic tools which are rapid, reliable, and have simple experimental protocols can significantly help in decreasing the prevalence rate of MDR-TB strain. We report the evaluation of the 9G technology based 9G DNAChips that allow accurate detection and discrimination of TB and MDR-TB-RIF. One hundred and thirteen known cultured samples were used to evaluate the ability of 9G DNAChip in the detection and discrimination of TB and MDR-TB-RIF strains. Hybridization of immobilized probes with the PCR products of TB and MDR-TB-RIF strains allow their detection and discrimination. The accuracy of 9G DNAChip was determined by comparing its results with sequencing analysis and drug susceptibility testing. Sequencing analysis showed 100% agreement with the results of 9G DNAChip. The 9G DNAChip showed very high sensitivity (95.4%) and specificity (100%).http://www.mdpi.com/1424-8220/16/3/376tuberculosisdrug-resistant tuberculosisdiagnosisaccuracysensitivityspecificity |
| spellingShingle | Keum-Soo Song Satish Balasaheb Nimse Hee Jin Kim Jeongseong Yang Taisun Kim Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains Sensors tuberculosis drug-resistant tuberculosis diagnosis accuracy sensitivity specificity |
| title | Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains |
| title_full | Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains |
| title_fullStr | Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains |
| title_full_unstemmed | Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains |
| title_short | Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains |
| title_sort | accurate detection of rifampicin resistant mycobacterium tuberculosis strains |
| topic | tuberculosis drug-resistant tuberculosis diagnosis accuracy sensitivity specificity |
| url | http://www.mdpi.com/1424-8220/16/3/376 |
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