The Doublesex/Mab-3 domain transcription factor DMD-10 regulates ASH-dependent behavioral responses
The Doublesex/Mab-3 Domain transcription factor DMD-10 is expressed in several cell types in C. elegans, including in the nervous system. We sought to investigate whether DMD-10 is required for normal neuronal function using behavioral assays. We found that mutation of dmd-10 did not broadly affect...
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PeerJ Inc.
2021-02-01
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| Online Access: | https://peerj.com/articles/10892.pdf |
| _version_ | 1797418894131986432 |
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| author | Julia Durbeck Celine Breton Michael Suter Eric S. Luth Annette M. McGehee |
| author_facet | Julia Durbeck Celine Breton Michael Suter Eric S. Luth Annette M. McGehee |
| author_sort | Julia Durbeck |
| collection | DOAJ |
| description | The Doublesex/Mab-3 Domain transcription factor DMD-10 is expressed in several cell types in C. elegans, including in the nervous system. We sought to investigate whether DMD-10 is required for normal neuronal function using behavioral assays. We found that mutation of dmd-10 did not broadly affect behavior. dmd-10 mutants were normal in several behavioral assays including a body bends assay for locomotion, egg laying, chemotaxis and response to gentle touch to the body. dmd-10 mutants did have defects in nose-touch responsiveness, which requires the glutamate receptor GLR-1. However, using quantitative fluorescence microscopy to measure levels of a GLR-1::GFP fusion protein in the ventral nerve cord, we found no evidence supporting a difference in the number of GLR-1 synapses or in the amount of GLR-1 present in dmd-10 mutants. dmd-10 mutants did have decreased responsiveness to high osmolarity, which, along with nose-touch, is sensed by the polymodal sensory neuron ASH. Furthermore, mutation of dmd-10 impaired behavioral response to optogenetic activation of ASH, suggesting that dmd-10 promotes neuronal signaling in ASH downstream of sensory receptor activation. Together our results suggest that DMD-10 is important in regulating the frequency of multiple ASH-dependent behavioral responses. |
| first_indexed | 2024-03-09T06:39:34Z |
| format | Article |
| id | doaj.art-bd70e83d53124c80ab4e3c963e063367 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:39:34Z |
| publishDate | 2021-02-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-bd70e83d53124c80ab4e3c963e0633672023-12-03T10:53:34ZengPeerJ Inc.PeerJ2167-83592021-02-019e1089210.7717/peerj.10892The Doublesex/Mab-3 domain transcription factor DMD-10 regulates ASH-dependent behavioral responsesJulia Durbeck0Celine Breton1Michael Suter2Eric S. Luth3Annette M. McGehee4Biology Department, Suffolk University, Boston, MA, USADepartment of Biology, Simmons University, Boston, MA, USABiology Department, Suffolk University, Boston, MA, USADepartment of Biology, Simmons University, Boston, MA, USABiology Department, Suffolk University, Boston, MA, USAThe Doublesex/Mab-3 Domain transcription factor DMD-10 is expressed in several cell types in C. elegans, including in the nervous system. We sought to investigate whether DMD-10 is required for normal neuronal function using behavioral assays. We found that mutation of dmd-10 did not broadly affect behavior. dmd-10 mutants were normal in several behavioral assays including a body bends assay for locomotion, egg laying, chemotaxis and response to gentle touch to the body. dmd-10 mutants did have defects in nose-touch responsiveness, which requires the glutamate receptor GLR-1. However, using quantitative fluorescence microscopy to measure levels of a GLR-1::GFP fusion protein in the ventral nerve cord, we found no evidence supporting a difference in the number of GLR-1 synapses or in the amount of GLR-1 present in dmd-10 mutants. dmd-10 mutants did have decreased responsiveness to high osmolarity, which, along with nose-touch, is sensed by the polymodal sensory neuron ASH. Furthermore, mutation of dmd-10 impaired behavioral response to optogenetic activation of ASH, suggesting that dmd-10 promotes neuronal signaling in ASH downstream of sensory receptor activation. Together our results suggest that DMD-10 is important in regulating the frequency of multiple ASH-dependent behavioral responses.https://peerj.com/articles/10892.pdfDMD-10C. elegansBehaviorASH |
| spellingShingle | Julia Durbeck Celine Breton Michael Suter Eric S. Luth Annette M. McGehee The Doublesex/Mab-3 domain transcription factor DMD-10 regulates ASH-dependent behavioral responses PeerJ DMD-10 C. elegans Behavior ASH |
| title | The Doublesex/Mab-3 domain transcription factor DMD-10 regulates ASH-dependent behavioral responses |
| title_full | The Doublesex/Mab-3 domain transcription factor DMD-10 regulates ASH-dependent behavioral responses |
| title_fullStr | The Doublesex/Mab-3 domain transcription factor DMD-10 regulates ASH-dependent behavioral responses |
| title_full_unstemmed | The Doublesex/Mab-3 domain transcription factor DMD-10 regulates ASH-dependent behavioral responses |
| title_short | The Doublesex/Mab-3 domain transcription factor DMD-10 regulates ASH-dependent behavioral responses |
| title_sort | doublesex mab 3 domain transcription factor dmd 10 regulates ash dependent behavioral responses |
| topic | DMD-10 C. elegans Behavior ASH |
| url | https://peerj.com/articles/10892.pdf |
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