Scalable downstream method for the cyclic lipopetide jagaricin
Abstract Cyclic lipopeptides are substances with a high potential to act as antimicrobial agents. Jagaricin, produced by Janthinobacterium agaricidamnosum DSM 9628 and discovered in 2012, is a new member of this class with promising antifungal properties. However, further experiments to investigate...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2022-12-01
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| Series: | Engineering in Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsc.202100079 |
| _version_ | 1811186249882402816 |
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| author | Jakob Stein Nicolas Schlosser Bettina Bardl Gundela Peschel Florian Meyer Florian Kloss Miriam A. Rosenbaum Lars Regestein |
| author_facet | Jakob Stein Nicolas Schlosser Bettina Bardl Gundela Peschel Florian Meyer Florian Kloss Miriam A. Rosenbaum Lars Regestein |
| author_sort | Jakob Stein |
| collection | DOAJ |
| description | Abstract Cyclic lipopeptides are substances with a high potential to act as antimicrobial agents. Jagaricin, produced by Janthinobacterium agaricidamnosum DSM 9628 and discovered in 2012, is a new member of this class with promising antifungal properties. However, further experiments to investigate future applications and/or conduct chemical derivatization to change properties and toxicity are impossible due to the limited access to jagaricin. Besides a high jagaricin concentration at the end of the fermentation process, a suitable downstream process is essential to generate appropriate amounts with the desired purity. In contrast to other amphiphilic molecules, jagaricin cannot be separated by foam fractionation since it is mainly attached to the surface of the microbial biomass. This technical report presents an overall process chain consisting of 11 individual steps to generate jagaricin in gram scale with a purity of over 95%. |
| first_indexed | 2024-04-11T13:42:18Z |
| format | Article |
| id | doaj.art-bd74ce99bd2b492ba1cc11672fc762af |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-04-11T13:42:18Z |
| publishDate | 2022-12-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-bd74ce99bd2b492ba1cc11672fc762af2022-12-22T04:21:12ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632022-12-01221281181710.1002/elsc.202100079Scalable downstream method for the cyclic lipopetide jagaricinJakob Stein0Nicolas Schlosser1Bettina Bardl2Gundela Peschel3Florian Meyer4Florian Kloss5Miriam A. Rosenbaum6Lars Regestein7Bio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyBio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyBio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyBio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyTransfer Group Anti‐Infectives Leibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyTransfer Group Anti‐Infectives Leibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyBio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyBio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyAbstract Cyclic lipopeptides are substances with a high potential to act as antimicrobial agents. Jagaricin, produced by Janthinobacterium agaricidamnosum DSM 9628 and discovered in 2012, is a new member of this class with promising antifungal properties. However, further experiments to investigate future applications and/or conduct chemical derivatization to change properties and toxicity are impossible due to the limited access to jagaricin. Besides a high jagaricin concentration at the end of the fermentation process, a suitable downstream process is essential to generate appropriate amounts with the desired purity. In contrast to other amphiphilic molecules, jagaricin cannot be separated by foam fractionation since it is mainly attached to the surface of the microbial biomass. This technical report presents an overall process chain consisting of 11 individual steps to generate jagaricin in gram scale with a purity of over 95%.https://doi.org/10.1002/elsc.202100079antifungalantimicrobialbiosurfactantJanthinobacterium agaricidamnosumlipopeptides |
| spellingShingle | Jakob Stein Nicolas Schlosser Bettina Bardl Gundela Peschel Florian Meyer Florian Kloss Miriam A. Rosenbaum Lars Regestein Scalable downstream method for the cyclic lipopetide jagaricin Engineering in Life Sciences antifungal antimicrobial biosurfactant Janthinobacterium agaricidamnosum lipopeptides |
| title | Scalable downstream method for the cyclic lipopetide jagaricin |
| title_full | Scalable downstream method for the cyclic lipopetide jagaricin |
| title_fullStr | Scalable downstream method for the cyclic lipopetide jagaricin |
| title_full_unstemmed | Scalable downstream method for the cyclic lipopetide jagaricin |
| title_short | Scalable downstream method for the cyclic lipopetide jagaricin |
| title_sort | scalable downstream method for the cyclic lipopetide jagaricin |
| topic | antifungal antimicrobial biosurfactant Janthinobacterium agaricidamnosum lipopeptides |
| url | https://doi.org/10.1002/elsc.202100079 |
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