Сomparison of Au, Au-Pt, and Au-Ag nanoparticles as markers for immunochromatographic determination of nonylphenol
Gold spherical nanoparticles, gold-platinum nanoflowers, and gold-silver nanostars were obtained and compared as labels for immunochromatographic analysis. The nanoparticles were synthesized by chemical reduction from various precursors and then conjugated with staphylococcal protein A to be used in...
Κύριοι συγγραφείς: | , , , , , |
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Μορφή: | Άρθρο |
Γλώσσα: | English |
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Uralʹskij federalʹnyj universitet imeni pervogo Prezidenta Rossii B.N. Elʹcina
2022-12-01
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Σειρά: | Chimica Techno Acta |
Θέματα: | |
Διαθέσιμο Online: | https://chimicatechnoacta.ru/article/view/6369 |
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author | Anna N. Berlina Maria Y. Ragozina Nadezhda S. Komova Kseniya V. Serebrennikova Anatoly V. Zherdev Boris B. Dzantiev |
author_facet | Anna N. Berlina Maria Y. Ragozina Nadezhda S. Komova Kseniya V. Serebrennikova Anatoly V. Zherdev Boris B. Dzantiev |
author_sort | Anna N. Berlina |
collection | DOAJ |
description | Gold spherical nanoparticles, gold-platinum nanoflowers, and gold-silver nanostars were obtained and compared as labels for immunochromatographic analysis. The nanoparticles were synthesized by chemical reduction from various precursors and then conjugated with staphylococcal protein A to be used in indirect immunochromatographic determination of nonylphenol. The results obtained were evaluated in terms of analytical characteristics and R2 value, as well as the color intensity of the test band. According to the comparison results, it was revealed that the R2 value varied from 0.82 for the gold-silver nanostars to 0.96 for the spherical gold nanoparticles. The working range of determined concentrations was from 2 to 100 μg/mL for unspherical and from 2 to 50 μg/mL – for spherical markers used; the analysis time was 20 min. |
first_indexed | 2024-04-09T17:13:03Z |
format | Article |
id | doaj.art-bd7693486c244dd1ac880a222a4b845e |
institution | Directory Open Access Journal |
issn | 2411-1414 |
language | English |
last_indexed | 2025-02-17T13:52:20Z |
publishDate | 2022-12-01 |
publisher | Uralʹskij federalʹnyj universitet imeni pervogo Prezidenta Rossii B.N. Elʹcina |
record_format | Article |
series | Chimica Techno Acta |
spelling | doaj.art-bd7693486c244dd1ac880a222a4b845e2024-12-25T09:36:55ZengUralʹskij federalʹnyj universitet imeni pervogo Prezidenta Rossii B.N. ElʹcinaChimica Techno Acta2411-14142022-12-0110110.15826/chimtech.2023.10.1.034735Сomparison of Au, Au-Pt, and Au-Ag nanoparticles as markers for immunochromatographic determination of nonylphenolAnna N. Berlina0Maria Y. Ragozina1Nadezhda S. Komova2Kseniya V. Serebrennikova3Anatoly V. Zherdev4Boris B. Dzantiev5A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, RussiaGold spherical nanoparticles, gold-platinum nanoflowers, and gold-silver nanostars were obtained and compared as labels for immunochromatographic analysis. The nanoparticles were synthesized by chemical reduction from various precursors and then conjugated with staphylococcal protein A to be used in indirect immunochromatographic determination of nonylphenol. The results obtained were evaluated in terms of analytical characteristics and R2 value, as well as the color intensity of the test band. According to the comparison results, it was revealed that the R2 value varied from 0.82 for the gold-silver nanostars to 0.96 for the spherical gold nanoparticles. The working range of determined concentrations was from 2 to 100 μg/mL for unspherical and from 2 to 50 μg/mL – for spherical markers used; the analysis time was 20 min.https://chimicatechnoacta.ru/article/view/6369gold nanoparticlesgold-platinum nanoflowersgold-silver nanostarsimmunochromatographic analysisnonylphenol |
spellingShingle | Anna N. Berlina Maria Y. Ragozina Nadezhda S. Komova Kseniya V. Serebrennikova Anatoly V. Zherdev Boris B. Dzantiev Сomparison of Au, Au-Pt, and Au-Ag nanoparticles as markers for immunochromatographic determination of nonylphenol Chimica Techno Acta gold nanoparticles gold-platinum nanoflowers gold-silver nanostars immunochromatographic analysis nonylphenol |
title | Сomparison of Au, Au-Pt, and Au-Ag nanoparticles as markers for immunochromatographic determination of nonylphenol |
title_full | Сomparison of Au, Au-Pt, and Au-Ag nanoparticles as markers for immunochromatographic determination of nonylphenol |
title_fullStr | Сomparison of Au, Au-Pt, and Au-Ag nanoparticles as markers for immunochromatographic determination of nonylphenol |
title_full_unstemmed | Сomparison of Au, Au-Pt, and Au-Ag nanoparticles as markers for immunochromatographic determination of nonylphenol |
title_short | Сomparison of Au, Au-Pt, and Au-Ag nanoparticles as markers for immunochromatographic determination of nonylphenol |
title_sort | сomparison of au au pt and au ag nanoparticles as markers for immunochromatographic determination of nonylphenol |
topic | gold nanoparticles gold-platinum nanoflowers gold-silver nanostars immunochromatographic analysis nonylphenol |
url | https://chimicatechnoacta.ru/article/view/6369 |
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