Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications
The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local de...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-06-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/6/761 |
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| author | Marius Rădulescu Alina Maria Holban Laurențiu Mogoantă Tudor-Adrian Bălşeanu George Dan Mogoșanu Diana Savu Roxana Cristina Popescu Oana Fufă Alexandru Mihai Grumezescu Eugenia Bezirtzoglou Veronica Lazar Mariana Carmen Chifiriuc |
| author_facet | Marius Rădulescu Alina Maria Holban Laurențiu Mogoantă Tudor-Adrian Bălşeanu George Dan Mogoșanu Diana Savu Roxana Cristina Popescu Oana Fufă Alexandru Mihai Grumezescu Eugenia Bezirtzoglou Veronica Lazar Mariana Carmen Chifiriuc |
| author_sort | Marius Rădulescu |
| collection | DOAJ |
| description | The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic. Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques. In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line. The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus). |
| first_indexed | 2024-12-20T12:48:49Z |
| format | Article |
| id | doaj.art-bd77aa397e8947b88a6fd13af989d588 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T12:48:49Z |
| publishDate | 2016-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-bd77aa397e8947b88a6fd13af989d5882022-12-21T19:40:14ZengMDPI AGMolecules1420-30492016-06-0121676110.3390/molecules21060761molecules21060761Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing ApplicationsMarius Rădulescu0Alina Maria Holban1Laurențiu Mogoantă2Tudor-Adrian Bălşeanu3George Dan Mogoșanu4Diana Savu5Roxana Cristina Popescu6Oana Fufă7Alexandru Mihai Grumezescu8Eugenia Bezirtzoglou9Veronica Lazar10Mariana Carmen Chifiriuc11Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, RomaniaDepartment of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, RomaniaResearch Center for Microscopic Morphology and Immunology, University of Medicine and Pharmacy of Craiova, PetruRares Street, No. 2, 200349 Craiova, RomaniaResearch Center for Clinical and Experimental Medicine, University of Medicine and Pharmacy of Craiova 2 PetruRareş Street, 200349 Craiova, RomaniaDepartment of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, PetruRares Street, No. 2, 200349 Craiova, RomaniaDepartment of Life and Environmental Physics, “HoriaHulubei” National Institute of Physics and Nuclear Engineering, Magurele, 077125 Bucharest, RomaniaDepartment of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, RomaniaDepartment of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, RomaniaDepartment of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, RomaniaLaboratory of Microbiology, Biotechnology and Hygiene, Department of Food Science and Technology, Faculty of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, GreeceMicrobiology Immunology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 5, 77206 Bucharest, RomaniaMicrobiology Immunology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 5, 77206 Bucharest, RomaniaThe aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic. Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques. In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line. The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).http://www.mdpi.com/1420-3049/21/6/761chitinsodium alginatedrug deliverycefepimecefuroximeL929 cell lineratsanti-biofilm |
| spellingShingle | Marius Rădulescu Alina Maria Holban Laurențiu Mogoantă Tudor-Adrian Bălşeanu George Dan Mogoșanu Diana Savu Roxana Cristina Popescu Oana Fufă Alexandru Mihai Grumezescu Eugenia Bezirtzoglou Veronica Lazar Mariana Carmen Chifiriuc Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications Molecules chitin sodium alginate drug delivery cefepime cefuroxime L929 cell line rats anti-biofilm |
| title | Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications |
| title_full | Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications |
| title_fullStr | Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications |
| title_full_unstemmed | Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications |
| title_short | Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications |
| title_sort | fabrication characterization and evaluation of bionanocomposites based on natural polymers and antibiotics for wound healing applications |
| topic | chitin sodium alginate drug delivery cefepime cefuroxime L929 cell line rats anti-biofilm |
| url | http://www.mdpi.com/1420-3049/21/6/761 |
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