Study of In Vitro and In Vivo Carbamazepine Release from Coarse and Nanometric Pharmaceutical Emulsions Obtained via Ultra-High-Pressure Homogenization
In the past decade, pharmaceutical nanotechnology has proven to be a promising alternative for improving the physicochemical and biopharmaceutical features for conventional pharmaceutical drug formulations. The goal of this study was to develop, characterize, and evaluate the in vitro and in vivo re...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-03-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/13/4/53 |
| _version_ | 1818317031268679680 |
|---|---|
| author | Juan D. Echeverri Maria J. Alhajj Nicolle Montero Cristhian J. Yarce Alvaro Barrera-Ocampo Constain H. Salamanca |
| author_facet | Juan D. Echeverri Maria J. Alhajj Nicolle Montero Cristhian J. Yarce Alvaro Barrera-Ocampo Constain H. Salamanca |
| author_sort | Juan D. Echeverri |
| collection | DOAJ |
| description | In the past decade, pharmaceutical nanotechnology has proven to be a promising alternative for improving the physicochemical and biopharmaceutical features for conventional pharmaceutical drug formulations. The goal of this study was to develop, characterize, and evaluate the in vitro and in vivo release of the model drug carbamazepine (CBZ) from two emulsified formulations with different droplet sizes (coarse and nanometric). Briefly, oil-in-water emulsions were developed using (i) Sacha inchi oil, ultrapure water, Tween<sup>TM</sup> 80, and Span<sup>TM</sup> 80 as surfactants, (ii) methyl-paraben and propyl-paraben as preservatives, and (iii) CBZ as a nonpolar model drug. The coarse and nanometric emulsions were prepared by rotor−stator dispersion and ultra-high-pressure homogenization (UHPH), respectively. The in vitro drug release studies were conducted by dialysis, whereas the in vivo drug release was evaluated in New Zealand breed rabbits. The results showed that nanoemulsions were physically more stable than coarse emulsions, and that CBZ had a very low release for in vitro determination (<2%), and a release of 20% in the in vivo study. However, it was found that nanoemulsions could significantly increase drug absorption time from 12 h to 45 min. |
| first_indexed | 2024-12-13T09:30:51Z |
| format | Article |
| id | doaj.art-bd7aace3ed8942f58cf810b412f7b4ad |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-12-13T09:30:51Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-bd7aace3ed8942f58cf810b412f7b4ad2022-12-21T23:52:30ZengMDPI AGPharmaceuticals1424-82472020-03-011345310.3390/ph13040053ph13040053Study of In Vitro and In Vivo Carbamazepine Release from Coarse and Nanometric Pharmaceutical Emulsions Obtained via Ultra-High-Pressure HomogenizationJuan D. Echeverri0Maria J. Alhajj1Nicolle Montero2Cristhian J. Yarce3Alvaro Barrera-Ocampo4Constain H. Salamanca5Programa de Maestría en Formulación de Productos Químicos y Derivados, Facultad de Ciencias Naturales, Universidad Icesi, Calle 18 No. 122-135, Cali 76003, ColombiaLaboratorio de Diseño y Formulación de Productos Químicos y Derivados, Facultad de Ciencias Naturales. Universidad Icesi, Calle 18 No. 122-135, Cali 76003, ColombiaLaboratorio de Diseño y Formulación de Productos Químicos y Derivados, Facultad de Ciencias Naturales. Universidad Icesi, Calle 18 No. 122-135, Cali 76003, ColombiaLaboratorio de Diseño y Formulación de Productos Químicos y Derivados, Facultad de Ciencias Naturales. Universidad Icesi, Calle 18 No. 122-135, Cali 76003, ColombiaDepartamento de Ciencias Farmacéuticas, Facultad de Ciencias Naturales, Universidad Icesi, Calle 18 No. 122-135, Cali 76003, ColombiaPrograma de Maestría en Formulación de Productos Químicos y Derivados, Facultad de Ciencias Naturales, Universidad Icesi, Calle 18 No. 122-135, Cali 76003, ColombiaIn the past decade, pharmaceutical nanotechnology has proven to be a promising alternative for improving the physicochemical and biopharmaceutical features for conventional pharmaceutical drug formulations. The goal of this study was to develop, characterize, and evaluate the in vitro and in vivo release of the model drug carbamazepine (CBZ) from two emulsified formulations with different droplet sizes (coarse and nanometric). Briefly, oil-in-water emulsions were developed using (i) Sacha inchi oil, ultrapure water, Tween<sup>TM</sup> 80, and Span<sup>TM</sup> 80 as surfactants, (ii) methyl-paraben and propyl-paraben as preservatives, and (iii) CBZ as a nonpolar model drug. The coarse and nanometric emulsions were prepared by rotor−stator dispersion and ultra-high-pressure homogenization (UHPH), respectively. The in vitro drug release studies were conducted by dialysis, whereas the in vivo drug release was evaluated in New Zealand breed rabbits. The results showed that nanoemulsions were physically more stable than coarse emulsions, and that CBZ had a very low release for in vitro determination (<2%), and a release of 20% in the in vivo study. However, it was found that nanoemulsions could significantly increase drug absorption time from 12 h to 45 min.https://www.mdpi.com/1424-8247/13/4/53carbamazepinecoarse emulsionin vitro/in vivo drug releasenanoemulsionultra-high-pressure homogenization |
| spellingShingle | Juan D. Echeverri Maria J. Alhajj Nicolle Montero Cristhian J. Yarce Alvaro Barrera-Ocampo Constain H. Salamanca Study of In Vitro and In Vivo Carbamazepine Release from Coarse and Nanometric Pharmaceutical Emulsions Obtained via Ultra-High-Pressure Homogenization Pharmaceuticals carbamazepine coarse emulsion in vitro/in vivo drug release nanoemulsion ultra-high-pressure homogenization |
| title | Study of In Vitro and In Vivo Carbamazepine Release from Coarse and Nanometric Pharmaceutical Emulsions Obtained via Ultra-High-Pressure Homogenization |
| title_full | Study of In Vitro and In Vivo Carbamazepine Release from Coarse and Nanometric Pharmaceutical Emulsions Obtained via Ultra-High-Pressure Homogenization |
| title_fullStr | Study of In Vitro and In Vivo Carbamazepine Release from Coarse and Nanometric Pharmaceutical Emulsions Obtained via Ultra-High-Pressure Homogenization |
| title_full_unstemmed | Study of In Vitro and In Vivo Carbamazepine Release from Coarse and Nanometric Pharmaceutical Emulsions Obtained via Ultra-High-Pressure Homogenization |
| title_short | Study of In Vitro and In Vivo Carbamazepine Release from Coarse and Nanometric Pharmaceutical Emulsions Obtained via Ultra-High-Pressure Homogenization |
| title_sort | study of in vitro and in vivo carbamazepine release from coarse and nanometric pharmaceutical emulsions obtained via ultra high pressure homogenization |
| topic | carbamazepine coarse emulsion in vitro/in vivo drug release nanoemulsion ultra-high-pressure homogenization |
| url | https://www.mdpi.com/1424-8247/13/4/53 |
| work_keys_str_mv | AT juandecheverri studyofinvitroandinvivocarbamazepinereleasefromcoarseandnanometricpharmaceuticalemulsionsobtainedviaultrahighpressurehomogenization AT mariajalhajj studyofinvitroandinvivocarbamazepinereleasefromcoarseandnanometricpharmaceuticalemulsionsobtainedviaultrahighpressurehomogenization AT nicollemontero studyofinvitroandinvivocarbamazepinereleasefromcoarseandnanometricpharmaceuticalemulsionsobtainedviaultrahighpressurehomogenization AT cristhianjyarce studyofinvitroandinvivocarbamazepinereleasefromcoarseandnanometricpharmaceuticalemulsionsobtainedviaultrahighpressurehomogenization AT alvarobarreraocampo studyofinvitroandinvivocarbamazepinereleasefromcoarseandnanometricpharmaceuticalemulsionsobtainedviaultrahighpressurehomogenization AT constainhsalamanca studyofinvitroandinvivocarbamazepinereleasefromcoarseandnanometricpharmaceuticalemulsionsobtainedviaultrahighpressurehomogenization |