Tracking Short-Range Attraction and Oviposition of European Grapevine Moths Affected by Volatile Organic Compounds in a Four-Chamber Olfactometer
The identification of volatile organic compounds (VOCs) leading to short-range attraction and oviposition of the European grapevine moth <i>Lobesia botrana</i> and European grape berry moth <i>Eupoecilia ambiguella</i> (Lepidoptera: Tortricidae) is crucial in order to establi...
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| Format: | Article |
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MDPI AG
2020-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/1/45 |
| _version_ | 1818234301041344512 |
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| author | Anna Markheiser Margit Rid Sandra Biancu Jürgen Gross Christoph Hoffmann |
| author_facet | Anna Markheiser Margit Rid Sandra Biancu Jürgen Gross Christoph Hoffmann |
| author_sort | Anna Markheiser |
| collection | DOAJ |
| description | The identification of volatile organic compounds (VOCs) leading to short-range attraction and oviposition of the European grapevine moth <i>Lobesia botrana</i> and European grape berry moth <i>Eupoecilia ambiguella</i> (Lepidoptera: Tortricidae) is crucial in order to establish bait-based decision support systems for control of these pests. Therefore, we developed a method to measure the real-time behavioral response of female moths to VOCs using a four-chamber olfactometer coupled with a video tracking system. Ten synthetic VOCs were selected for this study: (<i>S</i>)-(−)-perillaldehyde, (<i>E</i>)/(<i>Z</i>)-linalool oxide, (±)-limonene, linalool, (<i>E</i>)-β-caryophyllene, α/β-farnesene, (−)-α-cedrene, methyl salicylate and cumene. The effect of VOCs on egg deposition was determined using a dual-choice oviposition test, whereas perception by female antennae was verified using electroantennography (EAG). During video tracking, females responded to volatile compounds emitted by grapevine with higher antennae and ovipositor activity than to air control. (<i>E</i>)/(<i>Z</i>)-linalool oxide, cumene and (<i>S</i>)-(−)-perillaldehyde released ovipositor activity of <i>L. botrana</i>, while the latter provoked oviposition. (<i>R</i>)/(<i>S</i>)-limonene affected ovipositor activity of <i>E. ambiguella</i>, whereas none of the VOCs tested attracted for oviposition. The results suggest that females have the ability to perceive specific VOCs by the antennae but also by the ovipositor, which could attract or repel for egg deposition. |
| first_indexed | 2024-12-12T11:35:54Z |
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| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-12-12T11:35:54Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-bd822a59542440888716cc571c79fef42022-12-22T00:25:40ZengMDPI AGInsects2075-44502020-01-011114510.3390/insects11010045insects11010045Tracking Short-Range Attraction and Oviposition of European Grapevine Moths Affected by Volatile Organic Compounds in a Four-Chamber OlfactometerAnna Markheiser0Margit Rid1Sandra Biancu2Jürgen Gross3Christoph Hoffmann4Institute for Plant Protection in Fruit Crops and Viticulture—Zoology and Integrated Plant Protection, Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Geilweilerhof, 76833 Siebeldingen, GermanyInstitute for Plant Protection in Fruit Crops and Viticulture—Applied Chemical Ecology, Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Schwabenheimer Str. 101, 69221 Dossenheim, GermanyInstitute for Plant Protection in Fruit Crops and Viticulture—Zoology and Integrated Plant Protection, Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Geilweilerhof, 76833 Siebeldingen, GermanyInstitute for Plant Protection in Fruit Crops and Viticulture—Applied Chemical Ecology, Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Schwabenheimer Str. 101, 69221 Dossenheim, GermanyInstitute for Plant Protection in Fruit Crops and Viticulture—Zoology and Integrated Plant Protection, Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Geilweilerhof, 76833 Siebeldingen, GermanyThe identification of volatile organic compounds (VOCs) leading to short-range attraction and oviposition of the European grapevine moth <i>Lobesia botrana</i> and European grape berry moth <i>Eupoecilia ambiguella</i> (Lepidoptera: Tortricidae) is crucial in order to establish bait-based decision support systems for control of these pests. Therefore, we developed a method to measure the real-time behavioral response of female moths to VOCs using a four-chamber olfactometer coupled with a video tracking system. Ten synthetic VOCs were selected for this study: (<i>S</i>)-(−)-perillaldehyde, (<i>E</i>)/(<i>Z</i>)-linalool oxide, (±)-limonene, linalool, (<i>E</i>)-β-caryophyllene, α/β-farnesene, (−)-α-cedrene, methyl salicylate and cumene. The effect of VOCs on egg deposition was determined using a dual-choice oviposition test, whereas perception by female antennae was verified using electroantennography (EAG). During video tracking, females responded to volatile compounds emitted by grapevine with higher antennae and ovipositor activity than to air control. (<i>E</i>)/(<i>Z</i>)-linalool oxide, cumene and (<i>S</i>)-(−)-perillaldehyde released ovipositor activity of <i>L. botrana</i>, while the latter provoked oviposition. (<i>R</i>)/(<i>S</i>)-limonene affected ovipositor activity of <i>E. ambiguella</i>, whereas none of the VOCs tested attracted for oviposition. The results suggest that females have the ability to perceive specific VOCs by the antennae but also by the ovipositor, which could attract or repel for egg deposition.https://www.mdpi.com/2075-4450/11/1/45<i>l. botrana</i><i>e. ambiguella</i>grape berry mothvochost plant choicebehavioregg depositioneagkairomonespheromones |
| spellingShingle | Anna Markheiser Margit Rid Sandra Biancu Jürgen Gross Christoph Hoffmann Tracking Short-Range Attraction and Oviposition of European Grapevine Moths Affected by Volatile Organic Compounds in a Four-Chamber Olfactometer Insects <i>l. botrana</i> <i>e. ambiguella</i> grape berry moth voc host plant choice behavior egg deposition eag kairomones pheromones |
| title | Tracking Short-Range Attraction and Oviposition of European Grapevine Moths Affected by Volatile Organic Compounds in a Four-Chamber Olfactometer |
| title_full | Tracking Short-Range Attraction and Oviposition of European Grapevine Moths Affected by Volatile Organic Compounds in a Four-Chamber Olfactometer |
| title_fullStr | Tracking Short-Range Attraction and Oviposition of European Grapevine Moths Affected by Volatile Organic Compounds in a Four-Chamber Olfactometer |
| title_full_unstemmed | Tracking Short-Range Attraction and Oviposition of European Grapevine Moths Affected by Volatile Organic Compounds in a Four-Chamber Olfactometer |
| title_short | Tracking Short-Range Attraction and Oviposition of European Grapevine Moths Affected by Volatile Organic Compounds in a Four-Chamber Olfactometer |
| title_sort | tracking short range attraction and oviposition of european grapevine moths affected by volatile organic compounds in a four chamber olfactometer |
| topic | <i>l. botrana</i> <i>e. ambiguella</i> grape berry moth voc host plant choice behavior egg deposition eag kairomones pheromones |
| url | https://www.mdpi.com/2075-4450/11/1/45 |
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