Egg Development After In Vitro Insemination in Japanese Quail (Coturnix japonica)
In vitro fertilization has been widely used to produce offspring in several mammalian species. We previously successfully produced Japanese quail chicks using intracytoplasmic sperm injection (ICSI), whereas in vitro insemination was not successful. This may be due to the difficulties associated wit...
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| Format: | Article |
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Japan Poultry Science Association
2023-01-01
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| Series: | The Journal of Poultry Science |
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| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/60/1/60_2023001/_article/-char/en |
| _version_ | 1827939445456764928 |
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| author | Yoshinobu Ichikawa Shusei Mizushima Noritaka Hirohashi Tomohiro Sasanami |
| author_facet | Yoshinobu Ichikawa Shusei Mizushima Noritaka Hirohashi Tomohiro Sasanami |
| author_sort | Yoshinobu Ichikawa |
| collection | DOAJ |
| description | In vitro fertilization has been widely used to produce offspring in several mammalian species. We previously successfully produced Japanese quail chicks using intracytoplasmic sperm injection (ICSI), whereas in vitro insemination was not successful. This may be due to the difficulties associated with mimicking the sperm-egg fusion process and subsequent events in physiological polyspermic fertilization in vitro. In the present study, we observed egg development after in vitro insemination and investigated the inactivation of metaphase-promoting factor (MPF) and cytostatic factor (CSF), which are downstream of the Ca2+ signaling pathway in the egg, due to fertilizing sperm. We found a sperm number-dependent increase in hole formation caused by sperm penetration of the perivitelline membrane, the extracellular coat surrounding the egg. Egg development was observed following in vitro insemination; however, the developmental rate and stages after 24-h culture were inferior to those of ICSI eggs, even when insemination was performed with a high number of sperm (2 × 104). We also noted the downregulation of inositol 1,4,5-trisphosphate receptor-1, ryanodine receptor-3, cyclin B1, and c-MOS, which are important regulatory components of MPF and CSF in the egg, which was dependent on the number of sperm used for insemination. However, the decreases observed in these components did not reach the levels observed in the ICSI eggs. Collectively, the present results suggest that a sperm number higher than 2 × 104 is required for the progression of the Ca2+ signaling pathway, which initiates subsequent egg development in Japanese quail. |
| first_indexed | 2024-03-13T08:54:38Z |
| format | Article |
| id | doaj.art-bd8286b92045454d9dd87991f6f82a06 |
| institution | Directory Open Access Journal |
| issn | 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:54:38Z |
| publishDate | 2023-01-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-bd8286b92045454d9dd87991f6f82a062023-05-29T05:46:26ZengJapan Poultry Science AssociationThe Journal of Poultry Science1349-04862023-01-0160117https://doi.org/10.2141/jpsa.2023001Egg Development After In Vitro Insemination in Japanese Quail (Coturnix japonica)Yoshinobu Ichikawa0Shusei Mizushima1Noritaka Hirohashi2Tomohiro Sasanami3Department of Applied Life Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka City, Shizuka 422-8529, JapanDepartment of Biological Sciences, Faculty of Science, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, Hokkaido 060-0810, JapanOki Marine Biological Station, Education and Research Center for Biological Resources, Faculty of Life and Environmental Science, Shimane University, Oki, Shimane 685-0024, JapanDepartment of Applied Life Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka City, Shizuka 422-8529, JapanIn vitro fertilization has been widely used to produce offspring in several mammalian species. We previously successfully produced Japanese quail chicks using intracytoplasmic sperm injection (ICSI), whereas in vitro insemination was not successful. This may be due to the difficulties associated with mimicking the sperm-egg fusion process and subsequent events in physiological polyspermic fertilization in vitro. In the present study, we observed egg development after in vitro insemination and investigated the inactivation of metaphase-promoting factor (MPF) and cytostatic factor (CSF), which are downstream of the Ca2+ signaling pathway in the egg, due to fertilizing sperm. We found a sperm number-dependent increase in hole formation caused by sperm penetration of the perivitelline membrane, the extracellular coat surrounding the egg. Egg development was observed following in vitro insemination; however, the developmental rate and stages after 24-h culture were inferior to those of ICSI eggs, even when insemination was performed with a high number of sperm (2 × 104). We also noted the downregulation of inositol 1,4,5-trisphosphate receptor-1, ryanodine receptor-3, cyclin B1, and c-MOS, which are important regulatory components of MPF and CSF in the egg, which was dependent on the number of sperm used for insemination. However, the decreases observed in these components did not reach the levels observed in the ICSI eggs. Collectively, the present results suggest that a sperm number higher than 2 × 104 is required for the progression of the Ca2+ signaling pathway, which initiates subsequent egg development in Japanese quail.https://www.jstage.jst.go.jp/article/jpsa/60/1/60_2023001/_article/-char/enblastodermcytostatic factorin vitro inseminationjapanese quailmetaphase-promoting factor |
| spellingShingle | Yoshinobu Ichikawa Shusei Mizushima Noritaka Hirohashi Tomohiro Sasanami Egg Development After In Vitro Insemination in Japanese Quail (Coturnix japonica) The Journal of Poultry Science blastoderm cytostatic factor in vitro insemination japanese quail metaphase-promoting factor |
| title | Egg Development After In Vitro Insemination in Japanese Quail (Coturnix japonica) |
| title_full | Egg Development After In Vitro Insemination in Japanese Quail (Coturnix japonica) |
| title_fullStr | Egg Development After In Vitro Insemination in Japanese Quail (Coturnix japonica) |
| title_full_unstemmed | Egg Development After In Vitro Insemination in Japanese Quail (Coturnix japonica) |
| title_short | Egg Development After In Vitro Insemination in Japanese Quail (Coturnix japonica) |
| title_sort | egg development after in vitro insemination in japanese quail coturnix japonica |
| topic | blastoderm cytostatic factor in vitro insemination japanese quail metaphase-promoting factor |
| url | https://www.jstage.jst.go.jp/article/jpsa/60/1/60_2023001/_article/-char/en |
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