Molecular characterization of heat shock protein 70 gene in Iraqi buffalo
The heat shock protein 70 (HSP 70) has important roles in protecting cells and keeping them alive when exposed to different stress conditions. The polymorphism of the <em>hsp 70 </em>gene could be linked with the ability of stress tolerance. This study aimed to determine the polymorphism...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2020-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://vetmedmosul.com/article_164343_a17ee9e70a7bb4209cbb609b19ea71a2.pdf |
| _version_ | 1828524912871997440 |
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| author | Hassan N. Habib |
| author_facet | Hassan N. Habib |
| author_sort | Hassan N. Habib |
| collection | DOAJ |
| description | The heat shock protein 70 (HSP 70) has important roles in protecting cells and keeping them alive when exposed to different stress conditions. The polymorphism of the <em>hsp 70 </em>gene could be linked with the ability of stress tolerance. This study aimed to determine the polymorphism of the <em>hsp 70 </em>gene in Iraqi buffaloes and study bits effects on the resistance to stress. This study was conducted during from November 2018 to February 2019. The number of buffalo females used was 35 at the age of 4 - 6 years, which belonged to the local farmers from Basra city, Iraq. The DNA was extraction from the blood samples then the polymerase chain reaction (PCR) amplification was performed. The DNA sequences were analyzed by using bioinformatics analysis. The results of the molecular analysis showed that there were two groups of the <em>hsp 70 </em>gene as a compare with the same genes in GenBank due to silent and missense mutations. Based on these findings, it can be concluded that the Iraqi buffaloes have adapted to the surrounding environmental conditions as a result of the action of HSP 70 proliferation. The <em>hsp 70 </em>gene was a useful biomarker of stress tolerance in buffaloes. |
| first_indexed | 2024-12-11T20:57:44Z |
| format | Article |
| id | doaj.art-bd83195f3a924aaf999a57540ce7bdc4 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-12-11T20:57:44Z |
| publishDate | 2020-01-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-bd83195f3a924aaf999a57540ce7bdc42022-12-22T00:51:05ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552020-01-0134113914310.33899/ijvs.2019.125633.1116164343Molecular characterization of heat shock protein 70 gene in Iraqi buffaloHassan N. Habib0Department of Animal Production, College of Agriculture, University of Basra, IraqThe heat shock protein 70 (HSP 70) has important roles in protecting cells and keeping them alive when exposed to different stress conditions. The polymorphism of the <em>hsp 70 </em>gene could be linked with the ability of stress tolerance. This study aimed to determine the polymorphism of the <em>hsp 70 </em>gene in Iraqi buffaloes and study bits effects on the resistance to stress. This study was conducted during from November 2018 to February 2019. The number of buffalo females used was 35 at the age of 4 - 6 years, which belonged to the local farmers from Basra city, Iraq. The DNA was extraction from the blood samples then the polymerase chain reaction (PCR) amplification was performed. The DNA sequences were analyzed by using bioinformatics analysis. The results of the molecular analysis showed that there were two groups of the <em>hsp 70 </em>gene as a compare with the same genes in GenBank due to silent and missense mutations. Based on these findings, it can be concluded that the Iraqi buffaloes have adapted to the surrounding environmental conditions as a result of the action of HSP 70 proliferation. The <em>hsp 70 </em>gene was a useful biomarker of stress tolerance in buffaloes.https://vetmedmosul.com/article_164343_a17ee9e70a7bb4209cbb609b19ea71a2.pdfhsp 70 genebuffaloenvironmental stresspolymorphism |
| spellingShingle | Hassan N. Habib Molecular characterization of heat shock protein 70 gene in Iraqi buffalo Iraqi Journal of Veterinary Sciences hsp 70 gene buffalo environmental stress polymorphism |
| title | Molecular characterization of heat shock protein 70 gene in Iraqi buffalo |
| title_full | Molecular characterization of heat shock protein 70 gene in Iraqi buffalo |
| title_fullStr | Molecular characterization of heat shock protein 70 gene in Iraqi buffalo |
| title_full_unstemmed | Molecular characterization of heat shock protein 70 gene in Iraqi buffalo |
| title_short | Molecular characterization of heat shock protein 70 gene in Iraqi buffalo |
| title_sort | molecular characterization of heat shock protein 70 gene in iraqi buffalo |
| topic | hsp 70 gene buffalo environmental stress polymorphism |
| url | https://vetmedmosul.com/article_164343_a17ee9e70a7bb4209cbb609b19ea71a2.pdf |
| work_keys_str_mv | AT hassannhabib molecularcharacterizationofheatshockprotein70geneiniraqibuffalo |