First Report of Tripartite Symbiosis Potential among Soybean, <i>Bradyrhizobium japonicum</i>, and Dark Septate Endophytes
Successful soybean and rhizobium interaction is characterized by the formation of root nodules. This symbiosis significantly affects the growth and yield of soybeans and has long been recognized as a key factor in sustainable agricultural systems. Naturally, soybeans could form a tripartite symbioti...
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MDPI AG
2023-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/7/1788 |
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| author | Ni Luh Putu Citra Innosensia I Putu Wirya Suputra Gusti Ngurah Alit Susanta Wirya Kazuhiko Narisawa |
| author_facet | Ni Luh Putu Citra Innosensia I Putu Wirya Suputra Gusti Ngurah Alit Susanta Wirya Kazuhiko Narisawa |
| author_sort | Ni Luh Putu Citra Innosensia |
| collection | DOAJ |
| description | Successful soybean and rhizobium interaction is characterized by the formation of root nodules. This symbiosis significantly affects the growth and yield of soybeans and has long been recognized as a key factor in sustainable agricultural systems. Naturally, soybeans could form a tripartite symbiotic relationship with rhizobium and arbuscular mycorrhizal fungi (AMF). However, there is no information regarding the symbiotic potential of soybean, nodulating bacteria, and dark septate endophytic (DSE) fungi. This study aims to delve into new tripartite symbiosis potential, shedding light on its efficacy in improving soybean growth while reducing environmental impacts. We examined the effect of incorporating two DSEs: <i>Cladophialophora chaetospira</i> SK51 (Cc) and <i>Veronaeopsis simplex</i> Y34 (Vs), into the classic soybean-rhizobium symbiosis. Under sterile conditions, the co-inoculation of DSEs with <i>Bradyrhizobium japonicum</i> IncB6 (Bj) significantly increased the nodule number and dry weight, leading to the acceleration of soybean vegetative growth. Soybean nodule numbers under co-inoculation treatments-CcBj and VsBj-were significantly increased by 77.6 and 43.6%, respectively, compared to the Bj treatment. Furthermore, under the CcBj and VsBj treatments, the soybean nodule dry mass was significantly increased by 455 and 363%, respectively, compared to Bj. This finding represents the first report of new beneficial tripartite symbiosis potential for soybean nodulation and vegetative growth. |
| first_indexed | 2024-03-11T01:23:50Z |
| format | Article |
| id | doaj.art-bd84390943dd4d7487a47a1503de4fe9 |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T01:23:50Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-bd84390943dd4d7487a47a1503de4fe92023-11-18T17:56:11ZengMDPI AGAgronomy2073-43952023-07-01137178810.3390/agronomy13071788First Report of Tripartite Symbiosis Potential among Soybean, <i>Bradyrhizobium japonicum</i>, and Dark Septate EndophytesNi Luh Putu Citra Innosensia0I Putu Wirya Suputra1Gusti Ngurah Alit Susanta Wirya2Kazuhiko Narisawa3Department of Symbiotic Science of Environment and Natural Resources, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu 183-0054, JapanFaculty of Agriculture, Udayana University, Denpasar 80234, IndonesiaFaculty of Agriculture, Udayana University, Denpasar 80234, IndonesiaDepartment of Symbiotic Science of Environment and Natural Resources, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu 183-0054, JapanSuccessful soybean and rhizobium interaction is characterized by the formation of root nodules. This symbiosis significantly affects the growth and yield of soybeans and has long been recognized as a key factor in sustainable agricultural systems. Naturally, soybeans could form a tripartite symbiotic relationship with rhizobium and arbuscular mycorrhizal fungi (AMF). However, there is no information regarding the symbiotic potential of soybean, nodulating bacteria, and dark septate endophytic (DSE) fungi. This study aims to delve into new tripartite symbiosis potential, shedding light on its efficacy in improving soybean growth while reducing environmental impacts. We examined the effect of incorporating two DSEs: <i>Cladophialophora chaetospira</i> SK51 (Cc) and <i>Veronaeopsis simplex</i> Y34 (Vs), into the classic soybean-rhizobium symbiosis. Under sterile conditions, the co-inoculation of DSEs with <i>Bradyrhizobium japonicum</i> IncB6 (Bj) significantly increased the nodule number and dry weight, leading to the acceleration of soybean vegetative growth. Soybean nodule numbers under co-inoculation treatments-CcBj and VsBj-were significantly increased by 77.6 and 43.6%, respectively, compared to the Bj treatment. Furthermore, under the CcBj and VsBj treatments, the soybean nodule dry mass was significantly increased by 455 and 363%, respectively, compared to Bj. This finding represents the first report of new beneficial tripartite symbiosis potential for soybean nodulation and vegetative growth.https://www.mdpi.com/2073-4395/13/7/1788soybeandark septate endophytesnodulationco-inoculationplant-microbial interactions |
| spellingShingle | Ni Luh Putu Citra Innosensia I Putu Wirya Suputra Gusti Ngurah Alit Susanta Wirya Kazuhiko Narisawa First Report of Tripartite Symbiosis Potential among Soybean, <i>Bradyrhizobium japonicum</i>, and Dark Septate Endophytes Agronomy soybean dark septate endophytes nodulation co-inoculation plant-microbial interactions |
| title | First Report of Tripartite Symbiosis Potential among Soybean, <i>Bradyrhizobium japonicum</i>, and Dark Septate Endophytes |
| title_full | First Report of Tripartite Symbiosis Potential among Soybean, <i>Bradyrhizobium japonicum</i>, and Dark Septate Endophytes |
| title_fullStr | First Report of Tripartite Symbiosis Potential among Soybean, <i>Bradyrhizobium japonicum</i>, and Dark Septate Endophytes |
| title_full_unstemmed | First Report of Tripartite Symbiosis Potential among Soybean, <i>Bradyrhizobium japonicum</i>, and Dark Septate Endophytes |
| title_short | First Report of Tripartite Symbiosis Potential among Soybean, <i>Bradyrhizobium japonicum</i>, and Dark Septate Endophytes |
| title_sort | first report of tripartite symbiosis potential among soybean i bradyrhizobium japonicum i and dark septate endophytes |
| topic | soybean dark septate endophytes nodulation co-inoculation plant-microbial interactions |
| url | https://www.mdpi.com/2073-4395/13/7/1788 |
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