A Novel Variant of Avian Reovirus Is Pathogenic to Vaccinated Chickens
Avian reovirus (ARV) infections, characterized by severe arthritis, tenosynovitis, pericarditis, and poor weight gain, have become increasingly serious in recent years. The economic impact is significant as it causes growth inhibition and immunosuppression. Some commercial poultry in China have been...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/9/1800 |
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| author | Rui Liu Dan Luo Jinhui Gao Kai Li Changjun Liu Xiaole Qi Hongyu Cui Yanping Zhang Suyan Wang Xiaomei Wang Yulong Gao Li Gao |
| author_facet | Rui Liu Dan Luo Jinhui Gao Kai Li Changjun Liu Xiaole Qi Hongyu Cui Yanping Zhang Suyan Wang Xiaomei Wang Yulong Gao Li Gao |
| author_sort | Rui Liu |
| collection | DOAJ |
| description | Avian reovirus (ARV) infections, characterized by severe arthritis, tenosynovitis, pericarditis, and poor weight gain, have become increasingly serious in recent years. The economic impact is significant as it causes growth inhibition and immunosuppression. Some commercial poultry in China have been widely vaccinated with available ARV vaccines; however, infections continue to occur even after vaccination. This study aimed to isolate a novel variant, ARV-SD19/11103, from the joint tissues of infected broiler chickens vaccinated with ARV vaccines in Shandong Province. Genetic evolution analysis of the major protective antigen σC gene in ARVs showed that ARV-SD19/11103 was located in the genotype cluster I but not in the same sub-cluster as the S1133 vaccine strain. The amino acid sequence similarity between SD19/11103 and vaccine strains S1133, 1733, and 2408 was <80%. After analyzing the amino acid sequences of the σC protein, 33 amino acid differences were found between the new variant isolate and the vaccine strains. This novel variant showed obvious pathogenicity in specific pathogen-free chicken embryos and chicks and could cause serious disease in chickens vaccinated with commercially available ARV vaccines. Cross-neutralization experiments further demonstrated a significant antigenic difference between the novel variant and genotype cluster I ARV strains. The novel variant strain isolated in this study provides an important theoretical basis for understanding the prevalence and genetic evolutionary characteristics of ARV variant strains in our country. This study identified the causes of ARVs circulating and emphasizes the needs for developing new vaccines against novel ARV variants. |
| first_indexed | 2024-03-10T21:52:52Z |
| format | Article |
| id | doaj.art-bd857f75b57c4d14a792f51bb3f47cfc |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T21:52:52Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-bd857f75b57c4d14a792f51bb3f47cfc2023-11-19T13:21:49ZengMDPI AGViruses1999-49152023-08-01159180010.3390/v15091800A Novel Variant of Avian Reovirus Is Pathogenic to Vaccinated ChickensRui Liu0Dan Luo1Jinhui Gao2Kai Li3Changjun Liu4Xiaole Qi5Hongyu Cui6Yanping Zhang7Suyan Wang8Xiaomei Wang9Yulong Gao10Li Gao11Division of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDivision of Avian Immunosuppressive Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaAvian reovirus (ARV) infections, characterized by severe arthritis, tenosynovitis, pericarditis, and poor weight gain, have become increasingly serious in recent years. The economic impact is significant as it causes growth inhibition and immunosuppression. Some commercial poultry in China have been widely vaccinated with available ARV vaccines; however, infections continue to occur even after vaccination. This study aimed to isolate a novel variant, ARV-SD19/11103, from the joint tissues of infected broiler chickens vaccinated with ARV vaccines in Shandong Province. Genetic evolution analysis of the major protective antigen σC gene in ARVs showed that ARV-SD19/11103 was located in the genotype cluster I but not in the same sub-cluster as the S1133 vaccine strain. The amino acid sequence similarity between SD19/11103 and vaccine strains S1133, 1733, and 2408 was <80%. After analyzing the amino acid sequences of the σC protein, 33 amino acid differences were found between the new variant isolate and the vaccine strains. This novel variant showed obvious pathogenicity in specific pathogen-free chicken embryos and chicks and could cause serious disease in chickens vaccinated with commercially available ARV vaccines. Cross-neutralization experiments further demonstrated a significant antigenic difference between the novel variant and genotype cluster I ARV strains. The novel variant strain isolated in this study provides an important theoretical basis for understanding the prevalence and genetic evolutionary characteristics of ARV variant strains in our country. This study identified the causes of ARVs circulating and emphasizes the needs for developing new vaccines against novel ARV variants.https://www.mdpi.com/1999-4915/15/9/1800avian reovirusvariantpathogenicityimmunized chickens |
| spellingShingle | Rui Liu Dan Luo Jinhui Gao Kai Li Changjun Liu Xiaole Qi Hongyu Cui Yanping Zhang Suyan Wang Xiaomei Wang Yulong Gao Li Gao A Novel Variant of Avian Reovirus Is Pathogenic to Vaccinated Chickens Viruses avian reovirus variant pathogenicity immunized chickens |
| title | A Novel Variant of Avian Reovirus Is Pathogenic to Vaccinated Chickens |
| title_full | A Novel Variant of Avian Reovirus Is Pathogenic to Vaccinated Chickens |
| title_fullStr | A Novel Variant of Avian Reovirus Is Pathogenic to Vaccinated Chickens |
| title_full_unstemmed | A Novel Variant of Avian Reovirus Is Pathogenic to Vaccinated Chickens |
| title_short | A Novel Variant of Avian Reovirus Is Pathogenic to Vaccinated Chickens |
| title_sort | novel variant of avian reovirus is pathogenic to vaccinated chickens |
| topic | avian reovirus variant pathogenicity immunized chickens |
| url | https://www.mdpi.com/1999-4915/15/9/1800 |
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