An ribonuclease T2 family protein modulates Acinetobacter baumannii abiotic surface colonization.
Acinetobacter baumannii is an emerging bacterial pathogen of considerable medical concern. The organism's transmission and ability to cause disease has been associated with its propensity to colonize and form biofilms on abiotic surfaces in health care settings. To better understand the genetic...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3904860?pdf=render |
| _version_ | 1811211926550609920 |
|---|---|
| author | Anna C Jacobs Catlyn E Blanchard Seana C Catherman Paul M Dunman Yoshihiko Murata |
| author_facet | Anna C Jacobs Catlyn E Blanchard Seana C Catherman Paul M Dunman Yoshihiko Murata |
| author_sort | Anna C Jacobs |
| collection | DOAJ |
| description | Acinetobacter baumannii is an emerging bacterial pathogen of considerable medical concern. The organism's transmission and ability to cause disease has been associated with its propensity to colonize and form biofilms on abiotic surfaces in health care settings. To better understand the genetic determinants that affect biomaterial attachment, we performed a transposon mutagenesis analysis of abiotic surface-colonization using A. baumannii strain 98-37-09. Disruption of an RNase T2 family gene was found to limit the organism's ability to colonize polystyrene, polypropylene, glass, and stainless steel surfaces. DNA microarray analyses revealed that in comparison to wild type and complemented cells, the RNase T2 family mutant exhibited reduced expression of 29 genes, 15 of which are predicted to be associated with bacterial attachment and surface-associated motility. Motility assays confirmed that RNase T2 mutant displays a severe motility defect. Taken together, our results indicate that the RNase T2 family protein identified in this study is a positive regulator of A. baumannii's ability to colonize inanimate surfaces and motility. Moreover, the enzyme may be an effective target for the intervention of biomaterial colonization, and consequently limit the organism's transmission within the hospital setting. |
| first_indexed | 2024-04-12T05:21:40Z |
| format | Article |
| id | doaj.art-bd860a06e0884355b7882ca7388751ec |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T05:21:40Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bd860a06e0884355b7882ca7388751ec2022-12-22T03:46:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8572910.1371/journal.pone.0085729An ribonuclease T2 family protein modulates Acinetobacter baumannii abiotic surface colonization.Anna C JacobsCatlyn E BlanchardSeana C CathermanPaul M DunmanYoshihiko MurataAcinetobacter baumannii is an emerging bacterial pathogen of considerable medical concern. The organism's transmission and ability to cause disease has been associated with its propensity to colonize and form biofilms on abiotic surfaces in health care settings. To better understand the genetic determinants that affect biomaterial attachment, we performed a transposon mutagenesis analysis of abiotic surface-colonization using A. baumannii strain 98-37-09. Disruption of an RNase T2 family gene was found to limit the organism's ability to colonize polystyrene, polypropylene, glass, and stainless steel surfaces. DNA microarray analyses revealed that in comparison to wild type and complemented cells, the RNase T2 family mutant exhibited reduced expression of 29 genes, 15 of which are predicted to be associated with bacterial attachment and surface-associated motility. Motility assays confirmed that RNase T2 mutant displays a severe motility defect. Taken together, our results indicate that the RNase T2 family protein identified in this study is a positive regulator of A. baumannii's ability to colonize inanimate surfaces and motility. Moreover, the enzyme may be an effective target for the intervention of biomaterial colonization, and consequently limit the organism's transmission within the hospital setting.http://europepmc.org/articles/PMC3904860?pdf=render |
| spellingShingle | Anna C Jacobs Catlyn E Blanchard Seana C Catherman Paul M Dunman Yoshihiko Murata An ribonuclease T2 family protein modulates Acinetobacter baumannii abiotic surface colonization. PLoS ONE |
| title | An ribonuclease T2 family protein modulates Acinetobacter baumannii abiotic surface colonization. |
| title_full | An ribonuclease T2 family protein modulates Acinetobacter baumannii abiotic surface colonization. |
| title_fullStr | An ribonuclease T2 family protein modulates Acinetobacter baumannii abiotic surface colonization. |
| title_full_unstemmed | An ribonuclease T2 family protein modulates Acinetobacter baumannii abiotic surface colonization. |
| title_short | An ribonuclease T2 family protein modulates Acinetobacter baumannii abiotic surface colonization. |
| title_sort | ribonuclease t2 family protein modulates acinetobacter baumannii abiotic surface colonization |
| url | http://europepmc.org/articles/PMC3904860?pdf=render |
| work_keys_str_mv | AT annacjacobs anribonucleaset2familyproteinmodulatesacinetobacterbaumanniiabioticsurfacecolonization AT catlyneblanchard anribonucleaset2familyproteinmodulatesacinetobacterbaumanniiabioticsurfacecolonization AT seanaccatherman anribonucleaset2familyproteinmodulatesacinetobacterbaumanniiabioticsurfacecolonization AT paulmdunman anribonucleaset2familyproteinmodulatesacinetobacterbaumanniiabioticsurfacecolonization AT yoshihikomurata anribonucleaset2familyproteinmodulatesacinetobacterbaumanniiabioticsurfacecolonization AT annacjacobs ribonucleaset2familyproteinmodulatesacinetobacterbaumanniiabioticsurfacecolonization AT catlyneblanchard ribonucleaset2familyproteinmodulatesacinetobacterbaumanniiabioticsurfacecolonization AT seanaccatherman ribonucleaset2familyproteinmodulatesacinetobacterbaumanniiabioticsurfacecolonization AT paulmdunman ribonucleaset2familyproteinmodulatesacinetobacterbaumanniiabioticsurfacecolonization AT yoshihikomurata ribonucleaset2familyproteinmodulatesacinetobacterbaumanniiabioticsurfacecolonization |