Heat Shock Improves Random Spore Analysis in Diverse Strains of Saccharomyces cerevisiae

Heat Shock Improves Random Spore Analysis in Diverse Strains of Saccharomyces cerevisiae

Random spore analysis (RSA) is a classic method in yeast genetics that allows high-throughput purification of recombinant haploid spores following specific crosses. RSA typically involves a number of steps to induce sporulation, purge vegetative cells that fail to sporulate, and disrupt the ascus wa...

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Bibliographic Details
Main Authors: Molly K. Burke, Kaitlin M. McHugh, Ian C. Kutch
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Genetics
Subjects:
random spore analysis
heat shock
sporulation
genomics
multiparent mapping population
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2020.597482/full

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