Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice
Abstract Ultrasound is an acoustic wave which can noninvasively penetrate the skull to deep brain regions, enabling neuromodulation. However, conventional ultrasound’s spatial resolution is diffraction-limited and low-precision. Here, we report acoustic nanobubble-mediated ultrasound stimulation cap...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-46461-y |
| _version_ | 1797259174621478912 |
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| author | Xuandi Hou Jianing Jing Yizhou Jiang Xiaohui Huang Quanxiang Xian Ting Lei Jiejun Zhu Kin Fung Wong Xinyi Zhao Min Su Danni Li Langzhou Liu Zhihai Qiu Lei Sun |
| author_facet | Xuandi Hou Jianing Jing Yizhou Jiang Xiaohui Huang Quanxiang Xian Ting Lei Jiejun Zhu Kin Fung Wong Xinyi Zhao Min Su Danni Li Langzhou Liu Zhihai Qiu Lei Sun |
| author_sort | Xuandi Hou |
| collection | DOAJ |
| description | Abstract Ultrasound is an acoustic wave which can noninvasively penetrate the skull to deep brain regions, enabling neuromodulation. However, conventional ultrasound’s spatial resolution is diffraction-limited and low-precision. Here, we report acoustic nanobubble-mediated ultrasound stimulation capable of localizing ultrasound’s effects to only the desired brain region in male mice. By varying the delivery site of nanobubbles, ultrasound could activate specific regions of the mouse motor cortex, evoking EMG signaling and limb movement, and could also, separately, activate one of two nearby deep brain regions to elicit distinct behaviors (freezing or rotation). Sonicated neurons displayed reversible, low-latency calcium responses and increased c-Fos expression in the sub-millimeter-scale region with nanobubbles present. Ultrasound stimulation of the relevant region also modified depression-like behavior in a mouse model. We also provide evidence of a role for mechanosensitive ion channels. Altogether, our treatment scheme allows spatially-targetable, repeatable and temporally-precise activation of deep brain circuits for neuromodulation without needing genetic modification. |
| first_indexed | 2024-04-24T23:05:14Z |
| format | Article |
| id | doaj.art-bd8873157a9743d2b422bb48371f4024 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T23:05:14Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-bd8873157a9743d2b422bb48371f40242024-03-17T12:31:57ZengNature PortfolioNature Communications2041-17232024-03-0115111810.1038/s41467-024-46461-yNanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in miceXuandi Hou0Jianing Jing1Yizhou Jiang2Xiaohui Huang3Quanxiang Xian4Ting Lei5Jiejun Zhu6Kin Fung Wong7Xinyi Zhao8Min Su9Danni Li10Langzhou Liu11Zhihai Qiu12Lei Sun13Department of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityGuangdong Institute of Intelligence Science and Technology, HengqinDepartment of Biomedical Engineering, The Hong Kong Polytechnic UniversityAbstract Ultrasound is an acoustic wave which can noninvasively penetrate the skull to deep brain regions, enabling neuromodulation. However, conventional ultrasound’s spatial resolution is diffraction-limited and low-precision. Here, we report acoustic nanobubble-mediated ultrasound stimulation capable of localizing ultrasound’s effects to only the desired brain region in male mice. By varying the delivery site of nanobubbles, ultrasound could activate specific regions of the mouse motor cortex, evoking EMG signaling and limb movement, and could also, separately, activate one of two nearby deep brain regions to elicit distinct behaviors (freezing or rotation). Sonicated neurons displayed reversible, low-latency calcium responses and increased c-Fos expression in the sub-millimeter-scale region with nanobubbles present. Ultrasound stimulation of the relevant region also modified depression-like behavior in a mouse model. We also provide evidence of a role for mechanosensitive ion channels. Altogether, our treatment scheme allows spatially-targetable, repeatable and temporally-precise activation of deep brain circuits for neuromodulation without needing genetic modification.https://doi.org/10.1038/s41467-024-46461-y |
| spellingShingle | Xuandi Hou Jianing Jing Yizhou Jiang Xiaohui Huang Quanxiang Xian Ting Lei Jiejun Zhu Kin Fung Wong Xinyi Zhao Min Su Danni Li Langzhou Liu Zhihai Qiu Lei Sun Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice Nature Communications |
| title | Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice |
| title_full | Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice |
| title_fullStr | Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice |
| title_full_unstemmed | Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice |
| title_short | Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice |
| title_sort | nanobubble actuated ultrasound neuromodulation for selectively shaping behavior in mice |
| url | https://doi.org/10.1038/s41467-024-46461-y |
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