Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes
Donkeys are an important domesticated animal, providing labor, meat, milk, and medicinal materials for humans. However, the donkey population is continuously declining and even at risk of extinction. The application of modern animal production technology, such as oocyte in vitro maturation, is a pro...
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MDPI AG
2021-10-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/10/1640 |
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| author | Zhipeng Li Xinhui Song Shan Yin Jiageng Yan Peiru Lv Huiquan Shan Kuiqing Cui Hongbo Liu Qingyou Liu |
| author_facet | Zhipeng Li Xinhui Song Shan Yin Jiageng Yan Peiru Lv Huiquan Shan Kuiqing Cui Hongbo Liu Qingyou Liu |
| author_sort | Zhipeng Li |
| collection | DOAJ |
| description | Donkeys are an important domesticated animal, providing labor, meat, milk, and medicinal materials for humans. However, the donkey population is continuously declining and even at risk of extinction. The application of modern animal production technology, such as oocyte in vitro maturation, is a promising method to improve the donkey population. In this study, we explore the gene expression patterns of donkey germinal vesicle (GV) and in vitro matured metaphase II (MII) oocytes using single cell RNA-seq of the candidate genes along with the regulatory mechanisms that affect donkey oocyte maturation. We identified a total of 24,164 oocyte genes of which 9073 were significant differentially expressed in the GV and MII oocytes. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that these genes were associated with the meiotic cell cycle, mitochondrion activity, and N-glycan biosynthesis, which might be the key genes and regulatory mechanisms affecting the maturation of donkey oocytes. Our study provides considerable understanding regarding the maturation of donkey oocytes and serves as a theoretical basis for improving the development of donkey oocytes, which could ultimately benefit the expansion of the donkey population and conservation of biodiversity and genetic resources. |
| first_indexed | 2024-03-10T06:33:03Z |
| format | Article |
| id | doaj.art-bd888b976f7342b0babcca7663f2adc0 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T06:33:03Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-bd888b976f7342b0babcca7663f2adc02023-11-22T18:23:11ZengMDPI AGGenes2073-44252021-10-011210164010.3390/genes12101640Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey OocytesZhipeng Li0Xinhui Song1Shan Yin2Jiageng Yan3Peiru Lv4Huiquan Shan5Kuiqing Cui6Hongbo Liu7Qingyou Liu8Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Univesity, Nanning 530005, ChinaLaboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Univesity, Nanning 530005, ChinaHenan Chuangyuan Biotechnology Co. Ltd.; Zhengzhou 451100, ChinaLaboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Univesity, Nanning 530005, ChinaHenan Chuangyuan Biotechnology Co. Ltd.; Zhengzhou 451100, ChinaLaboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Univesity, Nanning 530005, ChinaLaboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Univesity, Nanning 530005, ChinaHenan Chuangyuan Biotechnology Co. Ltd.; Zhengzhou 451100, ChinaLaboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Univesity, Nanning 530005, ChinaDonkeys are an important domesticated animal, providing labor, meat, milk, and medicinal materials for humans. However, the donkey population is continuously declining and even at risk of extinction. The application of modern animal production technology, such as oocyte in vitro maturation, is a promising method to improve the donkey population. In this study, we explore the gene expression patterns of donkey germinal vesicle (GV) and in vitro matured metaphase II (MII) oocytes using single cell RNA-seq of the candidate genes along with the regulatory mechanisms that affect donkey oocyte maturation. We identified a total of 24,164 oocyte genes of which 9073 were significant differentially expressed in the GV and MII oocytes. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that these genes were associated with the meiotic cell cycle, mitochondrion activity, and N-glycan biosynthesis, which might be the key genes and regulatory mechanisms affecting the maturation of donkey oocytes. Our study provides considerable understanding regarding the maturation of donkey oocytes and serves as a theoretical basis for improving the development of donkey oocytes, which could ultimately benefit the expansion of the donkey population and conservation of biodiversity and genetic resources.https://www.mdpi.com/2073-4425/12/10/1640single-cell RNA-seqdonkeyoocytesin vitro maturationgene expression |
| spellingShingle | Zhipeng Li Xinhui Song Shan Yin Jiageng Yan Peiru Lv Huiquan Shan Kuiqing Cui Hongbo Liu Qingyou Liu Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes Genes single-cell RNA-seq donkey oocytes in vitro maturation gene expression |
| title | Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes |
| title_full | Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes |
| title_fullStr | Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes |
| title_full_unstemmed | Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes |
| title_short | Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes |
| title_sort | single cell rna seq revealed the gene expression pattern during the in vitro maturation of donkey oocytes |
| topic | single-cell RNA-seq donkey oocytes in vitro maturation gene expression |
| url | https://www.mdpi.com/2073-4425/12/10/1640 |
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