Effect of Epigallocatechin gallate (EGCG) on Production of Dendritic Cells from Peripheral Blood Monocytes
Background and purpose: Dendritic cells are professional antigen presenting cells that initiate and modulate immune responses. Epigallocatechin gallate (EGCG) is identified as a prophylactic agent that can suppress tumor formation. This research aimed at investigating the effect of EGCG on different...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2020-11-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-15190-en.html |
| _version_ | 1797958907953414144 |
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| author | Mohammad Ali Mofid Nakhaei Nabiallah Mohammadi Saeid Abediankenari |
| author_facet | Mohammad Ali Mofid Nakhaei Nabiallah Mohammadi Saeid Abediankenari |
| author_sort | Mohammad Ali Mofid Nakhaei |
| collection | DOAJ |
| description | Background and purpose: Dendritic cells are professional antigen presenting cells that initiate and modulate immune responses. Epigallocatechin gallate (EGCG) is identified as a prophylactic agent that can suppress tumor formation. This research aimed at investigating the effect of EGCG on differentiation of dendritic cells from monocytes and as a potential substitute for IL-4 in this process.
Materials and methods: In this experimental study, blood samples were drawn and peripheral blood mononuclear cells were isolated using FICOL. The monocytes CD14 + were purified by MACS. EGCG was added in 5 μmol / l and 10 μmol / l. On days six and eight, the cells were analyzed and differentiation factors such as CD83, HLA-DR, and CD-b were evaluated using flow cytometry. In addition, IL-23 and IL-35 levels were measured.
Results: CD-11 and CD11b levels in mature and immature dendritic cells were significantly different between the two paths (P<0.05), HLA-DR levels were also significantly different in dendritic cells (P<0.05). IL-23 and IL-35 levels in mature and immature dendritic cells were not significantly different, whereas these levels in mature dendritic cells showed significant differences between the two paths (P<0.05).
Conclusion: This study showed that EGCG compared to IL-4 can initiate monocyte differentiation to dendritic cells and this molecule can turn monocytes to dendritic cells with tolerogenic qualities compared to the classic path. |
| first_indexed | 2024-04-11T00:25:26Z |
| format | Article |
| id | doaj.art-bd89302783b74c18a07ea97de6de677f |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-11T00:25:26Z |
| publishDate | 2020-11-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-bd89302783b74c18a07ea97de6de677f2023-01-08T08:47:39ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792020-11-0130190110Effect of Epigallocatechin gallate (EGCG) on Production of Dendritic Cells from Peripheral Blood MonocytesMohammad Ali Mofid Nakhaei0Nabiallah Mohammadi1Saeid Abediankenari2 MSc Student in Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran PhD Student in Immunology, Immunogenetics Research center, Department of Immunology, Mazandaran University of Medical Sciences, Sari, Iran Professor, Immunogenetics Research center, Department of Immunology, Mazandaran University of Medical Sciences, Sari, Iran Background and purpose: Dendritic cells are professional antigen presenting cells that initiate and modulate immune responses. Epigallocatechin gallate (EGCG) is identified as a prophylactic agent that can suppress tumor formation. This research aimed at investigating the effect of EGCG on differentiation of dendritic cells from monocytes and as a potential substitute for IL-4 in this process. Materials and methods: In this experimental study, blood samples were drawn and peripheral blood mononuclear cells were isolated using FICOL. The monocytes CD14 + were purified by MACS. EGCG was added in 5 μmol / l and 10 μmol / l. On days six and eight, the cells were analyzed and differentiation factors such as CD83, HLA-DR, and CD-b were evaluated using flow cytometry. In addition, IL-23 and IL-35 levels were measured. Results: CD-11 and CD11b levels in mature and immature dendritic cells were significantly different between the two paths (P<0.05), HLA-DR levels were also significantly different in dendritic cells (P<0.05). IL-23 and IL-35 levels in mature and immature dendritic cells were not significantly different, whereas these levels in mature dendritic cells showed significant differences between the two paths (P<0.05). Conclusion: This study showed that EGCG compared to IL-4 can initiate monocyte differentiation to dendritic cells and this molecule can turn monocytes to dendritic cells with tolerogenic qualities compared to the classic path.http://jmums.mazums.ac.ir/article-1-15190-en.htmlegcgil-35il-23 |
| spellingShingle | Mohammad Ali Mofid Nakhaei Nabiallah Mohammadi Saeid Abediankenari Effect of Epigallocatechin gallate (EGCG) on Production of Dendritic Cells from Peripheral Blood Monocytes Journal of Mazandaran University of Medical Sciences egcg il-35 il-23 |
| title | Effect of Epigallocatechin gallate (EGCG) on Production of Dendritic Cells from Peripheral Blood Monocytes |
| title_full | Effect of Epigallocatechin gallate (EGCG) on Production of Dendritic Cells from Peripheral Blood Monocytes |
| title_fullStr | Effect of Epigallocatechin gallate (EGCG) on Production of Dendritic Cells from Peripheral Blood Monocytes |
| title_full_unstemmed | Effect of Epigallocatechin gallate (EGCG) on Production of Dendritic Cells from Peripheral Blood Monocytes |
| title_short | Effect of Epigallocatechin gallate (EGCG) on Production of Dendritic Cells from Peripheral Blood Monocytes |
| title_sort | effect of epigallocatechin gallate egcg on production of dendritic cells from peripheral blood monocytes |
| topic | egcg il-35 il-23 |
| url | http://jmums.mazums.ac.ir/article-1-15190-en.html |
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