Saccharification of agricultural residues by Streptomyces sp. and ethanol production from agro-waste mixture hydrolysate
The present work demonstrated the potential of different agro-waste mixtures to produce ethanol. The forest soil bacterium (Streptomyces sp.) was exploited for the saccharification of the agro-waste mixture formulated by extreme vertices mixture design and the hydrolysate produced by saccharificatio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | Waste Management Bulletin |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949750723000147 |
| _version_ | 1797237847532503040 |
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| author | Sarita Shrestha Chonlong Chio Janak Raj Khatiwada Ou Li Wensheng Qin |
| author_facet | Sarita Shrestha Chonlong Chio Janak Raj Khatiwada Ou Li Wensheng Qin |
| author_sort | Sarita Shrestha |
| collection | DOAJ |
| description | The present work demonstrated the potential of different agro-waste mixtures to produce ethanol. The forest soil bacterium (Streptomyces sp.) was exploited for the saccharification of the agro-waste mixture formulated by extreme vertices mixture design and the hydrolysate produced by saccharification was used for fermentation. The best formulation contained 43.33% orange peel, 33.33 % pumpkin pulp + seeds, and 23.33% pomegranate peel which exhibited significantly high reducing sugar (22.36 ± 0.54 mg/g dry weight) among all other mixtures. The hydrolysate of this mixture when supplemented with 2% w/v fructose produced a maximum of 7.86 ± 0.08% v/v ethanol by the yeast isolated from the brewer’s spent grains. Thus, easily available waste could be a promising source for yeast isolation and feedstock for ethanol production. Further, this study aids to reduce the risk of health, and environmental pollution, and developing the economy. |
| first_indexed | 2024-04-24T17:26:15Z |
| format | Article |
| id | doaj.art-bd8cf75f1c35405d9467e6bf287dc370 |
| institution | Directory Open Access Journal |
| issn | 2949-7507 |
| language | English |
| last_indexed | 2024-04-24T17:26:15Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Waste Management Bulletin |
| spelling | doaj.art-bd8cf75f1c35405d9467e6bf287dc3702024-03-28T06:40:20ZengElsevierWaste Management Bulletin2949-75072023-12-01132733Saccharification of agricultural residues by Streptomyces sp. and ethanol production from agro-waste mixture hydrolysateSarita Shrestha0Chonlong Chio1Janak Raj Khatiwada2Ou Li3Wensheng Qin4Department of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, Ontario P7B 5E1, CanadaDepartment of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, Ontario P7B 5E1, CanadaDepartment of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, Ontario P7B 5E1, CanadaDepartment of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, Ontario P7B 5E1, Canada; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaDepartment of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, Ontario P7B 5E1, Canada; Corresponding author.The present work demonstrated the potential of different agro-waste mixtures to produce ethanol. The forest soil bacterium (Streptomyces sp.) was exploited for the saccharification of the agro-waste mixture formulated by extreme vertices mixture design and the hydrolysate produced by saccharification was used for fermentation. The best formulation contained 43.33% orange peel, 33.33 % pumpkin pulp + seeds, and 23.33% pomegranate peel which exhibited significantly high reducing sugar (22.36 ± 0.54 mg/g dry weight) among all other mixtures. The hydrolysate of this mixture when supplemented with 2% w/v fructose produced a maximum of 7.86 ± 0.08% v/v ethanol by the yeast isolated from the brewer’s spent grains. Thus, easily available waste could be a promising source for yeast isolation and feedstock for ethanol production. Further, this study aids to reduce the risk of health, and environmental pollution, and developing the economy.http://www.sciencedirect.com/science/article/pii/S2949750723000147Streptomyces sp.Mixture formulationAgro-wasteSaccharification |
| spellingShingle | Sarita Shrestha Chonlong Chio Janak Raj Khatiwada Ou Li Wensheng Qin Saccharification of agricultural residues by Streptomyces sp. and ethanol production from agro-waste mixture hydrolysate Waste Management Bulletin Streptomyces sp. Mixture formulation Agro-waste Saccharification |
| title | Saccharification of agricultural residues by Streptomyces sp. and ethanol production from agro-waste mixture hydrolysate |
| title_full | Saccharification of agricultural residues by Streptomyces sp. and ethanol production from agro-waste mixture hydrolysate |
| title_fullStr | Saccharification of agricultural residues by Streptomyces sp. and ethanol production from agro-waste mixture hydrolysate |
| title_full_unstemmed | Saccharification of agricultural residues by Streptomyces sp. and ethanol production from agro-waste mixture hydrolysate |
| title_short | Saccharification of agricultural residues by Streptomyces sp. and ethanol production from agro-waste mixture hydrolysate |
| title_sort | saccharification of agricultural residues by streptomyces sp and ethanol production from agro waste mixture hydrolysate |
| topic | Streptomyces sp. Mixture formulation Agro-waste Saccharification |
| url | http://www.sciencedirect.com/science/article/pii/S2949750723000147 |
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