Co-expression analysis of Dehydrin upstream regulatory genes on sugarcane (Saccharum officinarum L.) under drought stress condition
Sugarcane plantations in Indonesia have been expanded and shifted to the marginal land characterized by long drought period, therefore, an attempt has been initiated to generate drought tolerance varieties through genetic engineering. It could be conducted by inserting the gene that involve in...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
indonesian research institute for biotechnology and bioindustry
2020-10-01
|
| Series: | Menara Perkebunan |
| Subjects: | |
| Online Access: | http://mp.iribb.org/index.php/mpjurnal/article/view/396/pdf |
| _version_ | 1818302133007548416 |
|---|---|
| author | Hayati Minarsih Jembar Pambudi Riza A Putranto |
| author_facet | Hayati Minarsih Jembar Pambudi Riza A Putranto |
| author_sort | Hayati Minarsih |
| collection | DOAJ |
| description | Sugarcane plantations in Indonesia have been
expanded and shifted to the marginal land
characterized by long drought period, therefore,
an attempt has been initiated to generate drought
tolerance varieties through genetic engineering. It
could be conducted by inserting the gene that
involve in plant adaptation response to drought
stress such as dehydrin (DHN) into sugarcane
genome. The promoter of sugarcane DHN gene
was isolated and transformed into sugarcane in the
previous research. This study aimed to
demonstrate the functionality of sugarcane DHN
promoter through expression analysis of DHN
regulatory genes that play a role in response to
drought stress. Expression analyses using RTqPCR
were also conducted on regulatory genes of
sugarcane that inserted by Pr-1DHNSo construct
treated with drought stress. The results showed
that the expressions of SoMYB, SoWRKY,
SoNAC, and SoDHN genes were escalated on
sugarcane 16 days after stress treatment ranging
from 353 to 4067 folds relatively to untreated
samples in which SoNAC gene showed the highest
expression. On the other hand, the analysis on
transgenic sugarcane carrying DHN promoter
construct showed SoNAC and SoDREB
expression increased after 72 hours under drought
stress. The expression values of SoNAC in
transgenic and non-transgenic plants under
drought condition were 4.79 and 4.99,
respectively. Meanwhile, the expression values of
SoDREB in transgenic and non-transgenic plants
under drought condition were 13.2 and 13.3,
respectively. The results of these experiments
showed that the promoter construct of Pr-1DHNSo
was induced by drought stress treatments
highlighting the regulation of several upstream
genes of SoDHN. |
| first_indexed | 2024-12-13T05:34:03Z |
| format | Article |
| id | doaj.art-bd8e5b821ab748eea379754bc4dcd21a |
| institution | Directory Open Access Journal |
| issn | 0125-9318 1858-3768 |
| language | English |
| last_indexed | 2024-12-13T05:34:03Z |
| publishDate | 2020-10-01 |
| publisher | indonesian research institute for biotechnology and bioindustry |
| record_format | Article |
| series | Menara Perkebunan |
| spelling | doaj.art-bd8e5b821ab748eea379754bc4dcd21a2022-12-21T23:57:59Zengindonesian research institute for biotechnology and bioindustryMenara Perkebunan0125-93181858-37682020-10-0188214115010.22302/iribb.jur.mp.v88i2.396Co-expression analysis of Dehydrin upstream regulatory genes on sugarcane (Saccharum officinarum L.) under drought stress conditionHayati Minarsih 0Jembar Pambudi1Riza A Putranto2Indonesian Research Institute for Biotechnology and BioindustryIPB UniversityIndonesian Research Institute for Biotechnology and BioindustrySugarcane plantations in Indonesia have been expanded and shifted to the marginal land characterized by long drought period, therefore, an attempt has been initiated to generate drought tolerance varieties through genetic engineering. It could be conducted by inserting the gene that involve in plant adaptation response to drought stress such as dehydrin (DHN) into sugarcane genome. The promoter of sugarcane DHN gene was isolated and transformed into sugarcane in the previous research. This study aimed to demonstrate the functionality of sugarcane DHN promoter through expression analysis of DHN regulatory genes that play a role in response to drought stress. Expression analyses using RTqPCR were also conducted on regulatory genes of sugarcane that inserted by Pr-1DHNSo construct treated with drought stress. The results showed that the expressions of SoMYB, SoWRKY, SoNAC, and SoDHN genes were escalated on sugarcane 16 days after stress treatment ranging from 353 to 4067 folds relatively to untreated samples in which SoNAC gene showed the highest expression. On the other hand, the analysis on transgenic sugarcane carrying DHN promoter construct showed SoNAC and SoDREB expression increased after 72 hours under drought stress. The expression values of SoNAC in transgenic and non-transgenic plants under drought condition were 4.79 and 4.99, respectively. Meanwhile, the expression values of SoDREB in transgenic and non-transgenic plants under drought condition were 13.2 and 13.3, respectively. The results of these experiments showed that the promoter construct of Pr-1DHNSo was induced by drought stress treatments highlighting the regulation of several upstream genes of SoDHN.http://mp.iribb.org/index.php/mpjurnal/article/view/396/pdfexpression analysistranscription factorsdhn promoter |
| spellingShingle | Hayati Minarsih Jembar Pambudi Riza A Putranto Co-expression analysis of Dehydrin upstream regulatory genes on sugarcane (Saccharum officinarum L.) under drought stress condition Menara Perkebunan expression analysis transcription factors dhn promoter |
| title | Co-expression analysis of Dehydrin upstream regulatory genes on sugarcane (Saccharum officinarum L.) under drought stress condition |
| title_full | Co-expression analysis of Dehydrin upstream regulatory genes on sugarcane (Saccharum officinarum L.) under drought stress condition |
| title_fullStr | Co-expression analysis of Dehydrin upstream regulatory genes on sugarcane (Saccharum officinarum L.) under drought stress condition |
| title_full_unstemmed | Co-expression analysis of Dehydrin upstream regulatory genes on sugarcane (Saccharum officinarum L.) under drought stress condition |
| title_short | Co-expression analysis of Dehydrin upstream regulatory genes on sugarcane (Saccharum officinarum L.) under drought stress condition |
| title_sort | co expression analysis of dehydrin upstream regulatory genes on sugarcane saccharum officinarum l under drought stress condition |
| topic | expression analysis transcription factors dhn promoter |
| url | http://mp.iribb.org/index.php/mpjurnal/article/view/396/pdf |
| work_keys_str_mv | AT hayatiminarsih coexpressionanalysisofdehydrinupstreamregulatorygenesonsugarcanesaccharumofficinarumlunderdroughtstresscondition AT jembarpambudi coexpressionanalysisofdehydrinupstreamregulatorygenesonsugarcanesaccharumofficinarumlunderdroughtstresscondition AT rizaaputranto coexpressionanalysisofdehydrinupstreamregulatorygenesonsugarcanesaccharumofficinarumlunderdroughtstresscondition |