Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 <it>Campylobacter </it>isolates from seven species including atypical campylobacters
<p>Abstract</p> <p>Background</p> <p>Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from <it>Campylobacter </it>organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to ge...
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| Format: | Article |
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BMC
2009-12-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/256 |
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| author | Millar Beverley C Moore John E Misawa Naoaki Kakinuma Yuki Tazumi Akihiro Matsuda Motoo |
| author_facet | Millar Beverley C Moore John E Misawa Naoaki Kakinuma Yuki Tazumi Akihiro Matsuda Motoo |
| author_sort | Millar Beverley C |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from <it>Campylobacter </it>organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions.</p> <p>Results</p> <p>Only <it>C. sputorum </it>biovar sputorum LMG7975 and fecalis LMG8531, LMG8534 and LMG6728 of a total of 204 <it>Campylobacter </it>isolates (n = 56 <it>C. jejuni</it>; n = 11 <it>C. coli</it>; n = 33 <it>C. fetus</it>; n = 43 <it>C. upsaliensis</it>; n = 30 <it>C. hyointestinalis</it>; n = 4 <it>C. sputorum </it>biovar sputorum; n = 5 <it>C. sputorum </it>biovar fecalis; n = 5 <it>C. sputorum </it>biovar paraureolyticus; n = 10 <it>C. concisus</it>; n = 7 <it>C. curvus</it>) were shown to carry IVSs in helix 25 region. <it>C. sputorum </it>biovar fecalis LMG8531 and LMG8534, interestingly, carried two different kinds of the 23S rRNA genes with and without the IVS, respectively. Consequently, in a total of 265 isolates of 269, including 65 <it>C. lari </it>isolates examined previously, the absence of IVSs was identified in the helix 25 region. In the helix 45 region, all the <it>C. hyointestinalis</it>, <it>C. sputorum </it>and <it>C. concisus </it>isolates were shown not to carry any IVSs. However, the 30 of 56 <it>C. jejuni </it>isolates (54%), 5 of 11 <it>C. coli </it>(45%), 25 of 33 <it>C. fetus </it>(76%), 30 of 43 <it>C. upsaliensis </it>(70%) and 6 of 7 <it>C. curvus </it>(90%) were shown to carry IVSs. In <it>C. jejuni </it>and <it>C. upsaliensis </it>isolates, two different kinds of the 23S rRNA genes were also identified to occur with and without IVSs in the helix 45 region, respectively.</p> <p>Conclusions</p> <p>Secondary structure models were also constructed with all the IVSs identified in the present study. In the purified RNA fractions from the isolates which carried the 16S or 23S rRNA genes with the IVSs, no 16S or 23S rRNA was evident, respectively.</p> |
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| spelling | doaj.art-bd8f5b846a734b93b0f06ea12cbf0e7e2022-12-21T22:09:55ZengBMCBMC Microbiology1471-21802009-12-019125610.1186/1471-2180-9-256Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 <it>Campylobacter </it>isolates from seven species including atypical campylobactersMillar Beverley CMoore John EMisawa NaoakiKakinuma YukiTazumi AkihiroMatsuda Motoo<p>Abstract</p> <p>Background</p> <p>Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from <it>Campylobacter </it>organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions.</p> <p>Results</p> <p>Only <it>C. sputorum </it>biovar sputorum LMG7975 and fecalis LMG8531, LMG8534 and LMG6728 of a total of 204 <it>Campylobacter </it>isolates (n = 56 <it>C. jejuni</it>; n = 11 <it>C. coli</it>; n = 33 <it>C. fetus</it>; n = 43 <it>C. upsaliensis</it>; n = 30 <it>C. hyointestinalis</it>; n = 4 <it>C. sputorum </it>biovar sputorum; n = 5 <it>C. sputorum </it>biovar fecalis; n = 5 <it>C. sputorum </it>biovar paraureolyticus; n = 10 <it>C. concisus</it>; n = 7 <it>C. curvus</it>) were shown to carry IVSs in helix 25 region. <it>C. sputorum </it>biovar fecalis LMG8531 and LMG8534, interestingly, carried two different kinds of the 23S rRNA genes with and without the IVS, respectively. Consequently, in a total of 265 isolates of 269, including 65 <it>C. lari </it>isolates examined previously, the absence of IVSs was identified in the helix 25 region. In the helix 45 region, all the <it>C. hyointestinalis</it>, <it>C. sputorum </it>and <it>C. concisus </it>isolates were shown not to carry any IVSs. However, the 30 of 56 <it>C. jejuni </it>isolates (54%), 5 of 11 <it>C. coli </it>(45%), 25 of 33 <it>C. fetus </it>(76%), 30 of 43 <it>C. upsaliensis </it>(70%) and 6 of 7 <it>C. curvus </it>(90%) were shown to carry IVSs. In <it>C. jejuni </it>and <it>C. upsaliensis </it>isolates, two different kinds of the 23S rRNA genes were also identified to occur with and without IVSs in the helix 45 region, respectively.</p> <p>Conclusions</p> <p>Secondary structure models were also constructed with all the IVSs identified in the present study. In the purified RNA fractions from the isolates which carried the 16S or 23S rRNA genes with the IVSs, no 16S or 23S rRNA was evident, respectively.</p>http://www.biomedcentral.com/1471-2180/9/256 |
| spellingShingle | Millar Beverley C Moore John E Misawa Naoaki Kakinuma Yuki Tazumi Akihiro Matsuda Motoo Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 <it>Campylobacter </it>isolates from seven species including atypical campylobacters BMC Microbiology |
| title | Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 <it>Campylobacter </it>isolates from seven species including atypical campylobacters |
| title_full | Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 <it>Campylobacter </it>isolates from seven species including atypical campylobacters |
| title_fullStr | Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 <it>Campylobacter </it>isolates from seven species including atypical campylobacters |
| title_full_unstemmed | Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 <it>Campylobacter </it>isolates from seven species including atypical campylobacters |
| title_short | Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 <it>Campylobacter </it>isolates from seven species including atypical campylobacters |
| title_sort | identification and characterization of intervening sequences within 23s rrna genes from more than 200 it campylobacter it isolates from seven species including atypical campylobacters |
| url | http://www.biomedcentral.com/1471-2180/9/256 |
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