Gamma globulin binding of hinokiflavone with anti-osteoporosis effects: A mechanistic study
Oxidative stress can trigger apoptosis and associated reduction of osteoblast activity. Hinokiflavone (HNK) with a biflavonoid-based structure might show potent antioxidant activity. However, before the application of this compound as a promising therapeutic bioactive material, its interaction with...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535224001497 |
| _version_ | 1827256636683583488 |
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| author | Zhen Zhang Jialang Zhang Baorong He Dingjun Hao |
| author_facet | Zhen Zhang Jialang Zhang Baorong He Dingjun Hao |
| author_sort | Zhen Zhang |
| collection | DOAJ |
| description | Oxidative stress can trigger apoptosis and associated reduction of osteoblast activity. Hinokiflavone (HNK) with a biflavonoid-based structure might show potent antioxidant activity. However, before the application of this compound as a promising therapeutic bioactive material, its interaction with blood proteins should be investigated to further reveal its pharmacokinetic and pharmacodynamic properties. Therefore, in this paper, the interaction of HNK with γ-globulin, one of the main blood proteins, was assessed. Then, the antioxidant properties of HNK against H2O2-induced osteoblast cytotoxicity in MC3T3-E1 cells were assessed. It was shown that HNK can potentially bind to γ-globulin mostly with the aid of hydrophilic forces, with a slight effect on the protein structure. It was then determined that HNK significantly recovered cell survival and alkaline phosphatase (ALP) activity and collagen-I content in MC3T3-E1 cells exposed to H2O2. In addition, HNK decreased the production of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) in MC3T3-E1 cells triggered by H2O2. Finally, HNK was shown to control the apoptosis induction in MC3T3-E1 cells triggered by H2O2 mediated by regulation of Bax, Bcl-2 and caspase-3. Taken together, these data demonstrated that HNK with promising blood protein binding properties can show significant protective effects in MC3T3-E1 cells mediated by inhibition of osteoblast dysfunction, oxidative stress, and apoptosis. |
| first_indexed | 2024-04-24T14:29:17Z |
| format | Article |
| id | doaj.art-bd90adfb2db94c738e72612f0d7da519 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2025-03-22T01:42:03Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-bd90adfb2db94c738e72612f0d7da5192024-05-09T04:35:40ZengElsevierArabian Journal of Chemistry1878-53522024-06-01176105747Gamma globulin binding of hinokiflavone with anti-osteoporosis effects: A mechanistic studyZhen Zhang0Jialang Zhang1Baorong He2Dingjun Hao3Department of Spine Surgery, Xi’an Honghui Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710000, ChinaDepartment of Spine Surgery, Xi’an Honghui Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710000, ChinaDepartment of Spine Surgery, Xi’an Honghui Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710000, ChinaCorresponding author at: Department of Spine Surgery, Xi’an Honghui Hospital, School of Medicine, Xi’an Jiaotong University, 555 Youyidonglu, Xi’an 710000, Shaanxi Province, China.; Department of Spine Surgery, Xi’an Honghui Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710000, ChinaOxidative stress can trigger apoptosis and associated reduction of osteoblast activity. Hinokiflavone (HNK) with a biflavonoid-based structure might show potent antioxidant activity. However, before the application of this compound as a promising therapeutic bioactive material, its interaction with blood proteins should be investigated to further reveal its pharmacokinetic and pharmacodynamic properties. Therefore, in this paper, the interaction of HNK with γ-globulin, one of the main blood proteins, was assessed. Then, the antioxidant properties of HNK against H2O2-induced osteoblast cytotoxicity in MC3T3-E1 cells were assessed. It was shown that HNK can potentially bind to γ-globulin mostly with the aid of hydrophilic forces, with a slight effect on the protein structure. It was then determined that HNK significantly recovered cell survival and alkaline phosphatase (ALP) activity and collagen-I content in MC3T3-E1 cells exposed to H2O2. In addition, HNK decreased the production of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) in MC3T3-E1 cells triggered by H2O2. Finally, HNK was shown to control the apoptosis induction in MC3T3-E1 cells triggered by H2O2 mediated by regulation of Bax, Bcl-2 and caspase-3. Taken together, these data demonstrated that HNK with promising blood protein binding properties can show significant protective effects in MC3T3-E1 cells mediated by inhibition of osteoblast dysfunction, oxidative stress, and apoptosis.http://www.sciencedirect.com/science/article/pii/S1878535224001497Hinokiflavoneγ-GlobulinInteractionOsteoblastOxidative stressApoptosis |
| spellingShingle | Zhen Zhang Jialang Zhang Baorong He Dingjun Hao Gamma globulin binding of hinokiflavone with anti-osteoporosis effects: A mechanistic study Arabian Journal of Chemistry Hinokiflavone γ-Globulin Interaction Osteoblast Oxidative stress Apoptosis |
| title | Gamma globulin binding of hinokiflavone with anti-osteoporosis effects: A mechanistic study |
| title_full | Gamma globulin binding of hinokiflavone with anti-osteoporosis effects: A mechanistic study |
| title_fullStr | Gamma globulin binding of hinokiflavone with anti-osteoporosis effects: A mechanistic study |
| title_full_unstemmed | Gamma globulin binding of hinokiflavone with anti-osteoporosis effects: A mechanistic study |
| title_short | Gamma globulin binding of hinokiflavone with anti-osteoporosis effects: A mechanistic study |
| title_sort | gamma globulin binding of hinokiflavone with anti osteoporosis effects a mechanistic study |
| topic | Hinokiflavone γ-Globulin Interaction Osteoblast Oxidative stress Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S1878535224001497 |
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