Intergeneric Hybrid from <i>Jatropha curcas</i> L. and <i>Ricinus communis</i> L.: Characterization and Polyploid Induction
<i>Jatropha curcas</i> L. (2n = 2× = 22) is increasingly attracting attention in the biodiesel industry for its oil. However, the cultivation of <i>J. curcas</i> L. is faced with numerous challenges unlike the cultivation of <i>Ricinus communis</i> L. (2n...
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MDPI AG
2019-06-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/8/2/50 |
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| author | Duangporn Premjet Abraham Kusi Obeng Anupan Kongbangkerd Siripong Premjet |
| author_facet | Duangporn Premjet Abraham Kusi Obeng Anupan Kongbangkerd Siripong Premjet |
| author_sort | Duangporn Premjet |
| collection | DOAJ |
| description | <i>Jatropha curcas</i> L. (2n = 2× = 22) is increasingly attracting attention in the biodiesel industry for its oil. However, the cultivation of <i>J. curcas</i> L. is faced with numerous challenges unlike the cultivation of <i>Ricinus communis</i> L. (2n = 2× = 20), a closely related species. The generation of an intergeneric hybrid between <i>J</i>. <i>curcas</i> L. and <i>R. communis</i> L. was investigated. Intergeneric hybrids were produced by hand crossing. Immature embryos were rescued, in vitro, from the hybrid seeds and cultured on an enriched Murashige and Skoog (MS) medium for a month. The plantlets produced were grown in sterile peat moss in plastic pots and covered with polyethylene for 30 days, after which they were transferred into cement potted soil. The hybridity and the genuineness of the hybrids were successfully confirmed using randomly amplified polymorphic DNA (RAPD) markers. The number of branches, stem diameter, and leaf size of the F<sub>1</sub> hybrids were similar to those of <i>J</i>. <i>curcas</i> L. while the plant height was similar to that of <i>R. communis</i> L. Young hybrids were treated with various concentrations (0%, 0.3%, 0.4%, and 0.5%) of colchicine to induce polyploids. The calli (JR6) treated with 0.3% colchicine recorded the highest tetraploid cell percentage (38.89%). A high tetraploid cell percentage (>50%) is significant in overcoming the problem of sterility after hybridization. |
| first_indexed | 2024-03-12T07:13:05Z |
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| id | doaj.art-bd9233368b23482995deb6bd972b2d46 |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-12T07:13:05Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj.art-bd9233368b23482995deb6bd972b2d462023-09-02T22:59:57ZengMDPI AGBiology2079-77372019-06-01825010.3390/biology8020050biology8020050Intergeneric Hybrid from <i>Jatropha curcas</i> L. and <i>Ricinus communis</i> L.: Characterization and Polyploid InductionDuangporn Premjet0Abraham Kusi Obeng1Anupan Kongbangkerd2Siripong Premjet3Center of Excellent in Research for Agricultural Biotechnology, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Muang, Phitsanulok 65000, ThailandDepartment of Biology, Faculty of Science, Naresuan University, Muang, Phitsanulok 65000, ThailandCenter of Excellent in Research for Agricultural Biotechnology, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Muang, Phitsanulok 65000, ThailandCenter of Excellent in Research for Agricultural Biotechnology, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Muang, Phitsanulok 65000, Thailand<i>Jatropha curcas</i> L. (2n = 2× = 22) is increasingly attracting attention in the biodiesel industry for its oil. However, the cultivation of <i>J. curcas</i> L. is faced with numerous challenges unlike the cultivation of <i>Ricinus communis</i> L. (2n = 2× = 20), a closely related species. The generation of an intergeneric hybrid between <i>J</i>. <i>curcas</i> L. and <i>R. communis</i> L. was investigated. Intergeneric hybrids were produced by hand crossing. Immature embryos were rescued, in vitro, from the hybrid seeds and cultured on an enriched Murashige and Skoog (MS) medium for a month. The plantlets produced were grown in sterile peat moss in plastic pots and covered with polyethylene for 30 days, after which they were transferred into cement potted soil. The hybridity and the genuineness of the hybrids were successfully confirmed using randomly amplified polymorphic DNA (RAPD) markers. The number of branches, stem diameter, and leaf size of the F<sub>1</sub> hybrids were similar to those of <i>J</i>. <i>curcas</i> L. while the plant height was similar to that of <i>R. communis</i> L. Young hybrids were treated with various concentrations (0%, 0.3%, 0.4%, and 0.5%) of colchicine to induce polyploids. The calli (JR6) treated with 0.3% colchicine recorded the highest tetraploid cell percentage (38.89%). A high tetraploid cell percentage (>50%) is significant in overcoming the problem of sterility after hybridization.https://www.mdpi.com/2079-7737/8/2/50embryo rescuegene introgression<i>Jatropha curcas</i> L.polyploidizationRAPD marker<i>Ricinus communis</i> L. |
| spellingShingle | Duangporn Premjet Abraham Kusi Obeng Anupan Kongbangkerd Siripong Premjet Intergeneric Hybrid from <i>Jatropha curcas</i> L. and <i>Ricinus communis</i> L.: Characterization and Polyploid Induction Biology embryo rescue gene introgression <i>Jatropha curcas</i> L. polyploidization RAPD marker <i>Ricinus communis</i> L. |
| title | Intergeneric Hybrid from <i>Jatropha curcas</i> L. and <i>Ricinus communis</i> L.: Characterization and Polyploid Induction |
| title_full | Intergeneric Hybrid from <i>Jatropha curcas</i> L. and <i>Ricinus communis</i> L.: Characterization and Polyploid Induction |
| title_fullStr | Intergeneric Hybrid from <i>Jatropha curcas</i> L. and <i>Ricinus communis</i> L.: Characterization and Polyploid Induction |
| title_full_unstemmed | Intergeneric Hybrid from <i>Jatropha curcas</i> L. and <i>Ricinus communis</i> L.: Characterization and Polyploid Induction |
| title_short | Intergeneric Hybrid from <i>Jatropha curcas</i> L. and <i>Ricinus communis</i> L.: Characterization and Polyploid Induction |
| title_sort | intergeneric hybrid from i jatropha curcas i l and i ricinus communis i l characterization and polyploid induction |
| topic | embryo rescue gene introgression <i>Jatropha curcas</i> L. polyploidization RAPD marker <i>Ricinus communis</i> L. |
| url | https://www.mdpi.com/2079-7737/8/2/50 |
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