Generation of Mutants from the 57B Region of <i>Drosophila melanogaster</i>
The 57B region of <i>Drosophila melanogaster</i> includes a cluster of the three homeobox genes <i>orthopedia</i> (<i>otp</i>), <i>Drosophila Retinal homeobox</i> (<i>DRx</i>), and <i>homeobrain</i> (<i>hbn</i>). In...
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MDPI AG
2023-11-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/11/2047 |
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| author | Eva Louise Steinmetz Sandra Noh Christine Klöppel Martin F. Fuhr Nicole Bach Mona Evelyn Raffael Kirsten Hildebrandt Fabienne Wittling Doris Jann Uwe Walldorf |
| author_facet | Eva Louise Steinmetz Sandra Noh Christine Klöppel Martin F. Fuhr Nicole Bach Mona Evelyn Raffael Kirsten Hildebrandt Fabienne Wittling Doris Jann Uwe Walldorf |
| author_sort | Eva Louise Steinmetz |
| collection | DOAJ |
| description | The 57B region of <i>Drosophila melanogaster</i> includes a cluster of the three homeobox genes <i>orthopedia</i> (<i>otp</i>), <i>Drosophila Retinal homeobox</i> (<i>DRx</i>), and <i>homeobrain</i> (<i>hbn</i>). In an attempt to isolate mutants for these genes, we performed an EMS mutagenesis and isolated lethal mutants from the 57B region, among them mutants for <i>otp</i>, <i>DRx</i>, and <i>hbn</i>. With the help of two newly generated deletions from the 57B region, we mapped additional mutants to specific chromosomal intervals and identified several of these mutants from the 57B region molecularly. In addition, we generated mutants for CG15651 and RIC-3 by gene targeting and mutants for the genes CG9344, CG15649, CG15650, and ND-B14.7 using the CRISPR/Cas9 system. We determined the lethality period during development for most isolated mutants. In total, we analysed alleles from nine different genes from the 57B region of <i>Drosophila</i>, which could now be used to further explore the functions of the corresponding genes in the future. |
| first_indexed | 2024-03-09T16:48:13Z |
| format | Article |
| id | doaj.art-bd93dce61f744c63a7da38c5cc2dd8ff |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T16:48:13Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-bd93dce61f744c63a7da38c5cc2dd8ff2023-11-24T14:43:56ZengMDPI AGGenes2073-44252023-11-011411204710.3390/genes14112047Generation of Mutants from the 57B Region of <i>Drosophila melanogaster</i>Eva Louise Steinmetz0Sandra Noh1Christine Klöppel2Martin F. Fuhr3Nicole Bach4Mona Evelyn Raffael5Kirsten Hildebrandt6Fabienne Wittling7Doris Jann8Uwe Walldorf9Developmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, GermanyDevelopmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, GermanyDevelopmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, GermanyDevelopmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, GermanyDevelopmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, GermanyDevelopmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, GermanyDevelopmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, GermanyDevelopmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, GermanyDevelopmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, GermanyDevelopmental Biology, ZHMB (Center of Human and Molecular Biology), Saarland University, Building 61, D-66421 Homburg, GermanyThe 57B region of <i>Drosophila melanogaster</i> includes a cluster of the three homeobox genes <i>orthopedia</i> (<i>otp</i>), <i>Drosophila Retinal homeobox</i> (<i>DRx</i>), and <i>homeobrain</i> (<i>hbn</i>). In an attempt to isolate mutants for these genes, we performed an EMS mutagenesis and isolated lethal mutants from the 57B region, among them mutants for <i>otp</i>, <i>DRx</i>, and <i>hbn</i>. With the help of two newly generated deletions from the 57B region, we mapped additional mutants to specific chromosomal intervals and identified several of these mutants from the 57B region molecularly. In addition, we generated mutants for CG15651 and RIC-3 by gene targeting and mutants for the genes CG9344, CG15649, CG15650, and ND-B14.7 using the CRISPR/Cas9 system. We determined the lethality period during development for most isolated mutants. In total, we analysed alleles from nine different genes from the 57B region of <i>Drosophila</i>, which could now be used to further explore the functions of the corresponding genes in the future.https://www.mdpi.com/2073-4425/14/11/204757B mutants57B deletionsgene targetingCRISPR/Cas9 |
| spellingShingle | Eva Louise Steinmetz Sandra Noh Christine Klöppel Martin F. Fuhr Nicole Bach Mona Evelyn Raffael Kirsten Hildebrandt Fabienne Wittling Doris Jann Uwe Walldorf Generation of Mutants from the 57B Region of <i>Drosophila melanogaster</i> Genes 57B mutants 57B deletions gene targeting CRISPR/Cas9 |
| title | Generation of Mutants from the 57B Region of <i>Drosophila melanogaster</i> |
| title_full | Generation of Mutants from the 57B Region of <i>Drosophila melanogaster</i> |
| title_fullStr | Generation of Mutants from the 57B Region of <i>Drosophila melanogaster</i> |
| title_full_unstemmed | Generation of Mutants from the 57B Region of <i>Drosophila melanogaster</i> |
| title_short | Generation of Mutants from the 57B Region of <i>Drosophila melanogaster</i> |
| title_sort | generation of mutants from the 57b region of i drosophila melanogaster i |
| topic | 57B mutants 57B deletions gene targeting CRISPR/Cas9 |
| url | https://www.mdpi.com/2073-4425/14/11/2047 |
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