Ovine relaxin family peptide receptor 2 (RXFP2) gene polymorphism – no association with cryptorchidism
The polymorphism in ovine relaxin family peptide receptor 2 gene and the relevance of earlier established human and mice cryptorchidism associated SNP’s in Mandya and Hassan Sheep was studied. Genomic DNA was extracted from 60 cryptorchid and 80 normal unrelated sheep. Two sets of primers were des...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-03-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/88035 |
| _version_ | 1797896701627858944 |
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| author | G S NAVEEN KUMAR C S NAGARAJA R NAGARAJA M R JAYASHANKAR M A SUNIL KUMAR |
| author_facet | G S NAVEEN KUMAR C S NAGARAJA R NAGARAJA M R JAYASHANKAR M A SUNIL KUMAR |
| author_sort | G S NAVEEN KUMAR |
| collection | DOAJ |
| description | The polymorphism in ovine relaxin family peptide receptor 2 gene and the relevance of earlier established human and mice cryptorchidism associated SNP’s in Mandya and Hassan Sheep was studied. Genomic DNA was extracted from 60 cryptorchid and 80 normal unrelated sheep. Two sets of primers were designed to amplify exon 8 and exon 12–13 regions of ovine RXFP2 gene. SSCP revealed no polymorphism at exon 8, exon 12 and exon 13 of ovine RXFP2 indicating absence of T222P in exon 8 and D294G in exon 12 as reported in human and mice cryptorchids respectively. A novel SNP (KF527573.1, 171T>A) in intron12 of ovine RXFP2 was observed. The sheep population studied was in Hardy Weinberg equilibrium for the genotypes of the SNP. The distribution of genotypes was significantly different for Hassan and Mandya sheep breeds. However, the SNP in both the breeds studied was not associated with the cryptorchid phenotype. |
| first_indexed | 2024-04-10T07:46:57Z |
| format | Article |
| id | doaj.art-bd9719645272426ca8aaecf8ca1d4c28 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:46:57Z |
| publishDate | 2019-03-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-bd9719645272426ca8aaecf8ca1d4c282023-02-23T10:34:53ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272019-03-0189310.56093/ijans.v89i3.88035Ovine relaxin family peptide receptor 2 (RXFP2) gene polymorphism – no association with cryptorchidismG S NAVEEN KUMAR0C S NAGARAJA1R NAGARAJA2M R JAYASHANKAR3M A SUNIL KUMAR4Associate Professor, Veterinary College, Hassan, Karnataka 573 202 IndiaProfessor, Veterinary College, Hassan, Karnataka 573 202 IndiaProfessor, Veterinary College, Hassan, Karnataka 573 202 IndiaRtd. Professor, Department of Animal Genetics and Breeding, Veterinary College, Bengaluru.Assistant Professor, Veterinary College, Hassan, Karnataka 573 202 IndiaThe polymorphism in ovine relaxin family peptide receptor 2 gene and the relevance of earlier established human and mice cryptorchidism associated SNP’s in Mandya and Hassan Sheep was studied. Genomic DNA was extracted from 60 cryptorchid and 80 normal unrelated sheep. Two sets of primers were designed to amplify exon 8 and exon 12–13 regions of ovine RXFP2 gene. SSCP revealed no polymorphism at exon 8, exon 12 and exon 13 of ovine RXFP2 indicating absence of T222P in exon 8 and D294G in exon 12 as reported in human and mice cryptorchids respectively. A novel SNP (KF527573.1, 171T>A) in intron12 of ovine RXFP2 was observed. The sheep population studied was in Hardy Weinberg equilibrium for the genotypes of the SNP. The distribution of genotypes was significantly different for Hassan and Mandya sheep breeds. However, the SNP in both the breeds studied was not associated with the cryptorchid phenotype.https://epubs.icar.org.in/index.php/IJAnS/article/view/88035CryptorchidismMandyaOvinePolymorphismRXFP2 |
| spellingShingle | G S NAVEEN KUMAR C S NAGARAJA R NAGARAJA M R JAYASHANKAR M A SUNIL KUMAR Ovine relaxin family peptide receptor 2 (RXFP2) gene polymorphism – no association with cryptorchidism Indian Journal of Animal Sciences Cryptorchidism Mandya Ovine Polymorphism RXFP2 |
| title | Ovine relaxin family peptide receptor 2 (RXFP2) gene polymorphism – no association with cryptorchidism |
| title_full | Ovine relaxin family peptide receptor 2 (RXFP2) gene polymorphism – no association with cryptorchidism |
| title_fullStr | Ovine relaxin family peptide receptor 2 (RXFP2) gene polymorphism – no association with cryptorchidism |
| title_full_unstemmed | Ovine relaxin family peptide receptor 2 (RXFP2) gene polymorphism – no association with cryptorchidism |
| title_short | Ovine relaxin family peptide receptor 2 (RXFP2) gene polymorphism – no association with cryptorchidism |
| title_sort | ovine relaxin family peptide receptor 2 rxfp2 gene polymorphism a€ no association with cryptorchidism |
| topic | Cryptorchidism Mandya Ovine Polymorphism RXFP2 |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/88035 |
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