Antiproliferative activity of standardized herbal phytopreparation from Asclepias subulata [version 2; peer review: 2 approved, 1 not approved]
Background: Several studies have shown that active compounds of Asclepias subulata (cardenolides) have antiproliferative effect on human cancer cells. Cardenolides isolated from A. subulata can be used as active chemical markers to elaborate phytopharmaceutical preparations. The aim of this work was...
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F1000 Research Ltd
2022-10-01
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| Online Access: | https://f1000research.com/articles/11-527/v2 |
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| author | Wagner Vilegas Carlos Velazquez Ramón Enrique Robles Zepeda Salvador Enrique Meneses Sagrero Luisa Alondra Rascón Valenzuela Olivia Valenzuela Antelo Francisco Humberto González Gutiérrez Marcelo J. Dias-Silva |
| author_facet | Wagner Vilegas Carlos Velazquez Ramón Enrique Robles Zepeda Salvador Enrique Meneses Sagrero Luisa Alondra Rascón Valenzuela Olivia Valenzuela Antelo Francisco Humberto González Gutiérrez Marcelo J. Dias-Silva |
| author_sort | Wagner Vilegas |
| collection | DOAJ |
| description | Background: Several studies have shown that active compounds of Asclepias subulata (cardenolides) have antiproliferative effect on human cancer cells. Cardenolides isolated from A. subulata can be used as active chemical markers to elaborate phytopharmaceutical preparations. The aim of this work was to evaluate the antiproliferative effect of a standardized extract of the aerial parts, based on Asclepias subulata cardenolides. Methods: Four standardized extracts were prepared by HPLC-DAD depending on the concentration of calotropin and the antiproliferative activity was measured for the MTT assay, on the A549, MCF-7, HeLa, PC3 and ARPE cell lines. The concentrations of calotropin used for the standardization of the extracts were 10, 7.6, 5 and 1 mg/dL. Results: Standardization of the A. subulata extract based on calotropin at 7.6 mg/g dry weight was achieved and the antiproliferative activity was evaluated over A549, HeLa and MCF-7 cell lines, obtaining proliferation percentages of 3.8 to 13.4%. Conclusions: The standardized extracts of A. subulata at different concentrations of calotropin showed antiproliferative activity against all the cell lines evaluated. The greatest effect was observed against the HeLa cell line. |
| first_indexed | 2024-04-09T19:43:33Z |
| format | Article |
| id | doaj.art-bd971c989fd0491b8a89dae983ed8927 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-04-09T19:43:33Z |
| publishDate | 2022-10-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-bd971c989fd0491b8a89dae983ed89272023-04-04T00:00:00ZengF1000 Research LtdF1000Research2046-14022022-10-0111139179Antiproliferative activity of standardized herbal phytopreparation from Asclepias subulata [version 2; peer review: 2 approved, 1 not approved]Wagner Vilegas0Carlos Velazquez1Ramón Enrique Robles Zepeda2https://orcid.org/0000-0002-5279-4020Salvador Enrique Meneses Sagrero3https://orcid.org/0000-0003-2846-3333Luisa Alondra Rascón Valenzuela4Olivia Valenzuela Antelo5Francisco Humberto González Gutiérrez6Marcelo J. Dias-Silva7https://orcid.org/0000-0002-8988-5532Universidade Estadual Paulista (UNESP), Sao Vicente, Sao Paulo, BrazilCiencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, 83000, MexicoCiencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, 83000, MexicoCiencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, 83000, MexicoCiencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, 83000, MexicoCiencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, 83000, MexicoCiencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, 83000, MexicoUniversidade Estadual Paulista (UNESP), Sao Vicente, Sao Paulo, BrazilBackground: Several studies have shown that active compounds of Asclepias subulata (cardenolides) have antiproliferative effect on human cancer cells. Cardenolides isolated from A. subulata can be used as active chemical markers to elaborate phytopharmaceutical preparations. The aim of this work was to evaluate the antiproliferative effect of a standardized extract of the aerial parts, based on Asclepias subulata cardenolides. Methods: Four standardized extracts were prepared by HPLC-DAD depending on the concentration of calotropin and the antiproliferative activity was measured for the MTT assay, on the A549, MCF-7, HeLa, PC3 and ARPE cell lines. The concentrations of calotropin used for the standardization of the extracts were 10, 7.6, 5 and 1 mg/dL. Results: Standardization of the A. subulata extract based on calotropin at 7.6 mg/g dry weight was achieved and the antiproliferative activity was evaluated over A549, HeLa and MCF-7 cell lines, obtaining proliferation percentages of 3.8 to 13.4%. Conclusions: The standardized extracts of A. subulata at different concentrations of calotropin showed antiproliferative activity against all the cell lines evaluated. The greatest effect was observed against the HeLa cell line.https://f1000research.com/articles/11-527/v2Asclepias subulate Calotropin Cardenolides Standardized extract Antiproliferative activityeng |
| spellingShingle | Wagner Vilegas Carlos Velazquez Ramón Enrique Robles Zepeda Salvador Enrique Meneses Sagrero Luisa Alondra Rascón Valenzuela Olivia Valenzuela Antelo Francisco Humberto González Gutiérrez Marcelo J. Dias-Silva Antiproliferative activity of standardized herbal phytopreparation from Asclepias subulata [version 2; peer review: 2 approved, 1 not approved] F1000Research Asclepias subulate Calotropin Cardenolides Standardized extract Antiproliferative activity eng |
| title | Antiproliferative activity of standardized herbal phytopreparation from Asclepias subulata [version 2; peer review: 2 approved, 1 not approved] |
| title_full | Antiproliferative activity of standardized herbal phytopreparation from Asclepias subulata [version 2; peer review: 2 approved, 1 not approved] |
| title_fullStr | Antiproliferative activity of standardized herbal phytopreparation from Asclepias subulata [version 2; peer review: 2 approved, 1 not approved] |
| title_full_unstemmed | Antiproliferative activity of standardized herbal phytopreparation from Asclepias subulata [version 2; peer review: 2 approved, 1 not approved] |
| title_short | Antiproliferative activity of standardized herbal phytopreparation from Asclepias subulata [version 2; peer review: 2 approved, 1 not approved] |
| title_sort | antiproliferative activity of standardized herbal phytopreparation from asclepias subulata version 2 peer review 2 approved 1 not approved |
| topic | Asclepias subulate Calotropin Cardenolides Standardized extract Antiproliferative activity eng |
| url | https://f1000research.com/articles/11-527/v2 |
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