Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching

Non-photochemical quenching (NPQ) is an important photoprotective mechanism in plants and algae. Although the process is extensively studied, little is known about its relationship with ultrastructural changes of the thylakoid membranes. In order to better understand this relationship, we studied th...

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Main Authors: Renáta Ünnep, Suman Paul, Ottó Zsiros, László Kovács, Noémi K. Székely, Gábor Steinbach, Marie-Sousai Appavou, Lionel Porcar, Alfred R. Holzwarth, Győző Garab, Gergely Nagy
Format: Article
Language:English
Published: The Royal Society 2020-09-01
Series:Open Biology
Subjects:
Online Access:https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.200144
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author Renáta Ünnep
Suman Paul
Ottó Zsiros
László Kovács
Noémi K. Székely
Gábor Steinbach
Marie-Sousai Appavou
Lionel Porcar
Alfred R. Holzwarth
Győző Garab
Gergely Nagy
author_facet Renáta Ünnep
Suman Paul
Ottó Zsiros
László Kovács
Noémi K. Székely
Gábor Steinbach
Marie-Sousai Appavou
Lionel Porcar
Alfred R. Holzwarth
Győző Garab
Gergely Nagy
author_sort Renáta Ünnep
collection DOAJ
description Non-photochemical quenching (NPQ) is an important photoprotective mechanism in plants and algae. Although the process is extensively studied, little is known about its relationship with ultrastructural changes of the thylakoid membranes. In order to better understand this relationship, we studied the effects of illumination on the organization of thylakoid membranes in Monstera deliciosa leaves. This evergreen species is known to exhibit very large NPQ and to possess giant grana with dozens of stacked thylakoids. It is thus ideally suited for small-angle neutron scattering measurements (SANS)—a non-invasive technique, which is capable of providing spatially and statistically averaged information on the periodicity of the thylakoid membranes and their rapid reorganizations in vivo. We show that NPQ-inducing illumination causes a strong decrease in the periodic order of granum thylakoid membranes. Development of NPQ and light-induced ultrastructural changes, as well as the relaxation processes, follow similar kinetic patterns. Surprisingly, whereas NPQ is suppressed by diuron, it impedes only the relaxation of the structural changes and not its formation, suggesting that structural changes do not cause but enable NPQ. We also demonstrate that the diminishment of SANS peak does not originate from light-induced redistribution and reorientation of chloroplasts inside the cells.
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spelling doaj.art-bd9b3516d0b54e9fb2ee41a99db2b1992022-12-21T17:32:53ZengThe Royal SocietyOpen Biology2046-24412020-09-0110910.1098/rsob.200144200144Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenchingRenáta ÜnnepSuman PaulOttó ZsirosLászló KovácsNoémi K. SzékelyGábor SteinbachMarie-Sousai AppavouLionel PorcarAlfred R. HolzwarthGyőző GarabGergely NagyNon-photochemical quenching (NPQ) is an important photoprotective mechanism in plants and algae. Although the process is extensively studied, little is known about its relationship with ultrastructural changes of the thylakoid membranes. In order to better understand this relationship, we studied the effects of illumination on the organization of thylakoid membranes in Monstera deliciosa leaves. This evergreen species is known to exhibit very large NPQ and to possess giant grana with dozens of stacked thylakoids. It is thus ideally suited for small-angle neutron scattering measurements (SANS)—a non-invasive technique, which is capable of providing spatially and statistically averaged information on the periodicity of the thylakoid membranes and their rapid reorganizations in vivo. We show that NPQ-inducing illumination causes a strong decrease in the periodic order of granum thylakoid membranes. Development of NPQ and light-induced ultrastructural changes, as well as the relaxation processes, follow similar kinetic patterns. Surprisingly, whereas NPQ is suppressed by diuron, it impedes only the relaxation of the structural changes and not its formation, suggesting that structural changes do not cause but enable NPQ. We also demonstrate that the diminishment of SANS peak does not originate from light-induced redistribution and reorientation of chloroplasts inside the cells.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.200144chloroplast thylakoid membranesgranalamellar repeat distancenon-photochemical quenchingsmall-angle neutron scattering
spellingShingle Renáta Ünnep
Suman Paul
Ottó Zsiros
László Kovács
Noémi K. Székely
Gábor Steinbach
Marie-Sousai Appavou
Lionel Porcar
Alfred R. Holzwarth
Győző Garab
Gergely Nagy
Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching
Open Biology
chloroplast thylakoid membranes
grana
lamellar repeat distance
non-photochemical quenching
small-angle neutron scattering
title Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching
title_full Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching
title_fullStr Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching
title_full_unstemmed Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching
title_short Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching
title_sort thylakoid membrane reorganizations revealed by small angle neutron scattering of monstera deliciosa leaves associated with non photochemical quenching
topic chloroplast thylakoid membranes
grana
lamellar repeat distance
non-photochemical quenching
small-angle neutron scattering
url https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.200144
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