Correlation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in Tehran
Background: Multidrug resistant K. pneumoniae, particularly the extended-spectrum β-lactamase (ESBL) producing strains, are often responsible for the failure of antibiotic treatment in nosocomial infections. Employing molecular methods to distinguish between ESBL and non-ESBL producing isolates...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2015-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5373/10651_CE(Ra)_F(Sh)_PF1(AJ_Sh)_PFA(AK)_PF2(PAG).pdf |
| Summary: | Background: Multidrug resistant K. pneumoniae, particularly
the extended-spectrum β-lactamase (ESBL) producing strains,
are often responsible for the failure of antibiotic treatment
in nosocomial infections. Employing molecular methods to
distinguish between ESBL and non-ESBL producing isolates
can help quick identification of these multidrug resistant
pathogens and thereby initiating appropriate antibiotic therapy.
The aim of this study was to employ RAPD-PCR to distinguish
the genetic fingerprints of ESBL producing clinical isolates of K.
pneumoniae from ESBL negative strains.
Materials and Methods: Antibacterial susceptibility of 104 K.
pneumoniae clinical isolates was determined to 13 antibacterial
agents by disc diffusion. ESBL production was measured by the
double disc synergy test followed by phenotypic confirmatory
tests. Genetic fingerprinting was carried out by RAPD-PCR.
Results: All isolates were susceptible to imipenem. Antibiotic
resistance rates were: piperacillin (100%), ceftazidime (62.5%),
cefotaxime (57.6%), aztreonam (52.8%), cefepime (51.9%),
kanamycin (50.9%), gentamicin (41.3%), ciprofloxacin (37.5%),
nitrofurantoin (30.6%), nalidixic acid (22.1%), piperacillin/
tazobactam (21.1%) and amikacin (9.6%). ESBL production
was observed in 14 isolates (13.4%). Genetic fingerprinting
performed on 43 isolates (14 ESBL positive and 29 ESBL
negative) by RAPD-PCR, showed that 46.5% of the isolates
belonged to a single profile (genotype 1), of which, the majority
(62.1%) were non-ESBL producers.
Conclusion: RAPD-PCR results showed heterogeneity among
the isolates. There was no association between ESBL production
with any specific genetic fingerprint. |
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| ISSN: | 2249-782X 0973-709X |