Correlation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in Tehran
Background: Multidrug resistant K. pneumoniae, particularly the extended-spectrum β-lactamase (ESBL) producing strains, are often responsible for the failure of antibiotic treatment in nosocomial infections. Employing molecular methods to distinguish between ESBL and non-ESBL producing isolates...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5373/10651_CE(Ra)_F(Sh)_PF1(AJ_Sh)_PFA(AK)_PF2(PAG).pdf |
| _version_ | 1818448524804620288 |
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| author | Fereshteh Eftekhar Parvaneh Nouri |
| author_facet | Fereshteh Eftekhar Parvaneh Nouri |
| author_sort | Fereshteh Eftekhar |
| collection | DOAJ |
| description | Background: Multidrug resistant K. pneumoniae, particularly
the extended-spectrum β-lactamase (ESBL) producing strains,
are often responsible for the failure of antibiotic treatment
in nosocomial infections. Employing molecular methods to
distinguish between ESBL and non-ESBL producing isolates
can help quick identification of these multidrug resistant
pathogens and thereby initiating appropriate antibiotic therapy.
The aim of this study was to employ RAPD-PCR to distinguish
the genetic fingerprints of ESBL producing clinical isolates of K.
pneumoniae from ESBL negative strains.
Materials and Methods: Antibacterial susceptibility of 104 K.
pneumoniae clinical isolates was determined to 13 antibacterial
agents by disc diffusion. ESBL production was measured by the
double disc synergy test followed by phenotypic confirmatory
tests. Genetic fingerprinting was carried out by RAPD-PCR.
Results: All isolates were susceptible to imipenem. Antibiotic
resistance rates were: piperacillin (100%), ceftazidime (62.5%),
cefotaxime (57.6%), aztreonam (52.8%), cefepime (51.9%),
kanamycin (50.9%), gentamicin (41.3%), ciprofloxacin (37.5%),
nitrofurantoin (30.6%), nalidixic acid (22.1%), piperacillin/
tazobactam (21.1%) and amikacin (9.6%). ESBL production
was observed in 14 isolates (13.4%). Genetic fingerprinting
performed on 43 isolates (14 ESBL positive and 29 ESBL
negative) by RAPD-PCR, showed that 46.5% of the isolates
belonged to a single profile (genotype 1), of which, the majority
(62.1%) were non-ESBL producers.
Conclusion: RAPD-PCR results showed heterogeneity among
the isolates. There was no association between ESBL production
with any specific genetic fingerprint. |
| first_indexed | 2024-12-14T20:20:53Z |
| format | Article |
| id | doaj.art-bd9b66dfae914d2da45f7ffd39af8b16 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-14T20:20:53Z |
| publishDate | 2015-01-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-bd9b66dfae914d2da45f7ffd39af8b162022-12-21T22:48:44ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-01-0191DC01DC0310.7860/JCDR/2015/10651.5373Correlation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in TehranFereshteh Eftekhar0Parvaneh Nouri1Associate Professor, Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Chamran Highway, Tehran, Iran.Associate Professor, Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Chamran Highway, Tehran, Iran.Background: Multidrug resistant K. pneumoniae, particularly the extended-spectrum β-lactamase (ESBL) producing strains, are often responsible for the failure of antibiotic treatment in nosocomial infections. Employing molecular methods to distinguish between ESBL and non-ESBL producing isolates can help quick identification of these multidrug resistant pathogens and thereby initiating appropriate antibiotic therapy. The aim of this study was to employ RAPD-PCR to distinguish the genetic fingerprints of ESBL producing clinical isolates of K. pneumoniae from ESBL negative strains. Materials and Methods: Antibacterial susceptibility of 104 K. pneumoniae clinical isolates was determined to 13 antibacterial agents by disc diffusion. ESBL production was measured by the double disc synergy test followed by phenotypic confirmatory tests. Genetic fingerprinting was carried out by RAPD-PCR. Results: All isolates were susceptible to imipenem. Antibiotic resistance rates were: piperacillin (100%), ceftazidime (62.5%), cefotaxime (57.6%), aztreonam (52.8%), cefepime (51.9%), kanamycin (50.9%), gentamicin (41.3%), ciprofloxacin (37.5%), nitrofurantoin (30.6%), nalidixic acid (22.1%), piperacillin/ tazobactam (21.1%) and amikacin (9.6%). ESBL production was observed in 14 isolates (13.4%). Genetic fingerprinting performed on 43 isolates (14 ESBL positive and 29 ESBL negative) by RAPD-PCR, showed that 46.5% of the isolates belonged to a single profile (genotype 1), of which, the majority (62.1%) were non-ESBL producers. Conclusion: RAPD-PCR results showed heterogeneity among the isolates. There was no association between ESBL production with any specific genetic fingerprint.https://jcdr.net/articles/PDF/5373/10651_CE(Ra)_F(Sh)_PF1(AJ_Sh)_PFA(AK)_PF2(PAG).pdfesblgenetic fingerprintingklebsiella pneumoniaemultidrug resistancenosocomial isolatesrapd-pcr |
| spellingShingle | Fereshteh Eftekhar Parvaneh Nouri Correlation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in Tehran Journal of Clinical and Diagnostic Research esbl genetic fingerprinting klebsiella pneumoniae multidrug resistance nosocomial isolates rapd-pcr |
| title | Correlation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in Tehran |
| title_full | Correlation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in Tehran |
| title_fullStr | Correlation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in Tehran |
| title_full_unstemmed | Correlation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in Tehran |
| title_short | Correlation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in Tehran |
| title_sort | correlation of rapd pcr profiles with esbl production in clinical isolates of klebsiella pneumoniae in tehran |
| topic | esbl genetic fingerprinting klebsiella pneumoniae multidrug resistance nosocomial isolates rapd-pcr |
| url | https://jcdr.net/articles/PDF/5373/10651_CE(Ra)_F(Sh)_PF1(AJ_Sh)_PFA(AK)_PF2(PAG).pdf |
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