Effect of metformin on sepsis-associated acute lung injury and gut microbiota in aged rats with sepsis
BackgroundRecent studies reported the association between the changes in gut microbiota and sepsis, but there is unclear for the gut microbes on aged sepsis is associated acute lung injury (SALI), and metformin treatment for the change in gut microbiota. This study aimed to investigate the effect of...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1139436/full |
| _version_ | 1811157648413818880 |
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| author | Youdong Wan Shuya Wang Yifan Niu Boyang Duo Yinshuang Liu Zhenzhen Lu Ruixue Zhu |
| author_facet | Youdong Wan Shuya Wang Yifan Niu Boyang Duo Yinshuang Liu Zhenzhen Lu Ruixue Zhu |
| author_sort | Youdong Wan |
| collection | DOAJ |
| description | BackgroundRecent studies reported the association between the changes in gut microbiota and sepsis, but there is unclear for the gut microbes on aged sepsis is associated acute lung injury (SALI), and metformin treatment for the change in gut microbiota. This study aimed to investigate the effect of metformin on gut microbiota and SALI in aged rats with sepsis. It also explored the therapeutic mechanism and the effect of metformin on aged rats with SALI.MethodsAged 20-21 months SD rats were categorized into three groups: sham-operated rats (AgS group), rats with cecal ligation and puncture (CLP)-induced sepsis (AgCLP group), and rats treated with metformin (100 mg/kg) orally 1 h after CLP treatment (AgMET group). We collected feces from rats and analyzed them by 16S rRNA sequencing. Further, the lung samples were collected for histological analysis and quantitative real-time PCR (qPCR) assay and so on.ResultsThis study showed that some pathological changes occurring in the lungs of aged rats, such as hemorrhage, edema, and inflammation, improved after metformin treatment; the number of hepatocyte death increased in the AgCLP group, and decreased in the AgMET group. Moreover, metformin relieved SALI inflammation and damage. Importantly, the gut microbiota composition among the three groups in aged SALI rats was different. In particular, the proportion of E. coli and K. pneumoniae was higher in AgCLP group rats than AgS group rats and AgMET group rats; while metformin could increase the proportion of Firmicutes, Lactobacillus, Ruminococcus_1 and Lactobacillus_johnsonii in aged SALI rats. Moreover, Prevotella_9, Klebsiella and Escherichia_Shigella were correlated positively with the inflammatory factor IL-1 in the lung tissues; Firmicutes was correlated negatively with the inflammatory factor IL-1 and IL-6 in the lung tissues.ConclusionsOur findings suggested that metformin could improve SALI and gut microbiota in aged rats, which could provide a potential therapeutic treatment for SALI in aged sepsis. |
| first_indexed | 2024-04-10T05:09:44Z |
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| id | doaj.art-bd9cb79f5f494124940ffb4cf465ad78 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-10T05:09:44Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-bd9cb79f5f494124940ffb4cf465ad782023-03-09T10:28:39ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-03-011310.3389/fcimb.2023.11394361139436Effect of metformin on sepsis-associated acute lung injury and gut microbiota in aged rats with sepsisYoudong Wan0Shuya Wang1Yifan Niu2Boyang Duo3Yinshuang Liu4Zhenzhen Lu5Ruixue Zhu6Department of Emergency Intensive Care Unit, The Affiliated Hospital of Qingdao University, Qingdao, ChinaClinical Medicine of Zhengzhou University, Zhengzhou, ChinaClinical Medicine of Zhengzhou University, Zhengzhou, ChinaClinical Medicine of Zhengzhou University, Zhengzhou, ChinaClinical Medicine of Zhengzhou University, Zhengzhou, ChinaClinical Medicine of Zhengzhou University, Zhengzhou, ChinaDepartment of Health Management, The Affiliated Hospital of Qingdao University, Qingdao, ChinaBackgroundRecent studies reported the association between the changes in gut microbiota and sepsis, but there is unclear for the gut microbes on aged sepsis is associated acute lung injury (SALI), and metformin treatment for the change in gut microbiota. This study aimed to investigate the effect of metformin on gut microbiota and SALI in aged rats with sepsis. It also explored the therapeutic mechanism and the effect of metformin on aged rats with SALI.MethodsAged 20-21 months SD rats were categorized into three groups: sham-operated rats (AgS group), rats with cecal ligation and puncture (CLP)-induced sepsis (AgCLP group), and rats treated with metformin (100 mg/kg) orally 1 h after CLP treatment (AgMET group). We collected feces from rats and analyzed them by 16S rRNA sequencing. Further, the lung samples were collected for histological analysis and quantitative real-time PCR (qPCR) assay and so on.ResultsThis study showed that some pathological changes occurring in the lungs of aged rats, such as hemorrhage, edema, and inflammation, improved after metformin treatment; the number of hepatocyte death increased in the AgCLP group, and decreased in the AgMET group. Moreover, metformin relieved SALI inflammation and damage. Importantly, the gut microbiota composition among the three groups in aged SALI rats was different. In particular, the proportion of E. coli and K. pneumoniae was higher in AgCLP group rats than AgS group rats and AgMET group rats; while metformin could increase the proportion of Firmicutes, Lactobacillus, Ruminococcus_1 and Lactobacillus_johnsonii in aged SALI rats. Moreover, Prevotella_9, Klebsiella and Escherichia_Shigella were correlated positively with the inflammatory factor IL-1 in the lung tissues; Firmicutes was correlated negatively with the inflammatory factor IL-1 and IL-6 in the lung tissues.ConclusionsOur findings suggested that metformin could improve SALI and gut microbiota in aged rats, which could provide a potential therapeutic treatment for SALI in aged sepsis.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1139436/fullgut microbiotametforminsepsissepsis-associated acute lung injury (SALI)aged rats |
| spellingShingle | Youdong Wan Shuya Wang Yifan Niu Boyang Duo Yinshuang Liu Zhenzhen Lu Ruixue Zhu Effect of metformin on sepsis-associated acute lung injury and gut microbiota in aged rats with sepsis Frontiers in Cellular and Infection Microbiology gut microbiota metformin sepsis sepsis-associated acute lung injury (SALI) aged rats |
| title | Effect of metformin on sepsis-associated acute lung injury and gut microbiota in aged rats with sepsis |
| title_full | Effect of metformin on sepsis-associated acute lung injury and gut microbiota in aged rats with sepsis |
| title_fullStr | Effect of metformin on sepsis-associated acute lung injury and gut microbiota in aged rats with sepsis |
| title_full_unstemmed | Effect of metformin on sepsis-associated acute lung injury and gut microbiota in aged rats with sepsis |
| title_short | Effect of metformin on sepsis-associated acute lung injury and gut microbiota in aged rats with sepsis |
| title_sort | effect of metformin on sepsis associated acute lung injury and gut microbiota in aged rats with sepsis |
| topic | gut microbiota metformin sepsis sepsis-associated acute lung injury (SALI) aged rats |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1139436/full |
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