Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control
Abstract Chemical control of disease vectoring mosquitoes Aedes albopictus and Aedes aegypti is costly, unsustainable, and increasingly ineffective due to the spread of insecticide resistance. The Sterile Insect Technique is a valuable alternative but is limited by slow, error-prone, and wasteful se...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-06-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-05030-7 |
| _version_ | 1827922858617077760 |
|---|---|
| author | Célia Lutrat Myriam Burckbuchler Roenick Proveti Olmo Rémy Beugnon Albin Fontaine Omar S. Akbari Rafael Argilés-Herrero Thierry Baldet Jérémy Bouyer Eric Marois |
| author_facet | Célia Lutrat Myriam Burckbuchler Roenick Proveti Olmo Rémy Beugnon Albin Fontaine Omar S. Akbari Rafael Argilés-Herrero Thierry Baldet Jérémy Bouyer Eric Marois |
| author_sort | Célia Lutrat |
| collection | DOAJ |
| description | Abstract Chemical control of disease vectoring mosquitoes Aedes albopictus and Aedes aegypti is costly, unsustainable, and increasingly ineffective due to the spread of insecticide resistance. The Sterile Insect Technique is a valuable alternative but is limited by slow, error-prone, and wasteful sex-separation methods. Here, we present four Genetic Sexing Strains (two for each Aedes species) based on fluorescence markers linked to the m and M sex loci, allowing for the isolation of transgenic males. Furthermore, we demonstrate how combining these sexing strains enables the production of non-transgenic males. In a mass-rearing facility, 100,000 first instar male larvae could be sorted in under 1.5 h with an estimated 0.01–0.1% female contamination on a single machine. Cost-efficiency analyses revealed that using these strains could result in important savings while setting up and running a mass-rearing facility. Altogether, these Genetic Sexing Strains should enable a major upscaling in control programmes against these important vectors. |
| first_indexed | 2024-03-13T04:48:46Z |
| format | Article |
| id | doaj.art-bd9eed7ffac44712987c4c5d9fe897b7 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-13T04:48:46Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-bd9eed7ffac44712987c4c5d9fe897b72023-06-18T11:22:16ZengNature PortfolioCommunications Biology2399-36422023-06-016111210.1038/s42003-023-05030-7Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic controlCélia Lutrat0Myriam Burckbuchler1Roenick Proveti Olmo2Rémy Beugnon3Albin Fontaine4Omar S. Akbari5Rafael Argilés-Herrero6Thierry Baldet7Jérémy Bouyer8Eric Marois9CIRAD, UMR ASTRECNRS UPR9022, INSERM U1257, Université de StrasbourgCNRS UPR9022, INSERM U1257, Université de StrasbourgGerman Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-LeipzigUnité Parasitologie et Entomologie, Département Microbiologie et maladies infectieuses, Institut de Recherche Biomédicale des Armées (IRBA)School of Biological Sciences, Department of Cell and Developmental Biology, University of CaliforniaCicindela LtdCIRAD, UMR ASTRECIRAD, UMR ASTRECNRS UPR9022, INSERM U1257, Université de StrasbourgAbstract Chemical control of disease vectoring mosquitoes Aedes albopictus and Aedes aegypti is costly, unsustainable, and increasingly ineffective due to the spread of insecticide resistance. The Sterile Insect Technique is a valuable alternative but is limited by slow, error-prone, and wasteful sex-separation methods. Here, we present four Genetic Sexing Strains (two for each Aedes species) based on fluorescence markers linked to the m and M sex loci, allowing for the isolation of transgenic males. Furthermore, we demonstrate how combining these sexing strains enables the production of non-transgenic males. In a mass-rearing facility, 100,000 first instar male larvae could be sorted in under 1.5 h with an estimated 0.01–0.1% female contamination on a single machine. Cost-efficiency analyses revealed that using these strains could result in important savings while setting up and running a mass-rearing facility. Altogether, these Genetic Sexing Strains should enable a major upscaling in control programmes against these important vectors.https://doi.org/10.1038/s42003-023-05030-7 |
| spellingShingle | Célia Lutrat Myriam Burckbuchler Roenick Proveti Olmo Rémy Beugnon Albin Fontaine Omar S. Akbari Rafael Argilés-Herrero Thierry Baldet Jérémy Bouyer Eric Marois Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control Communications Biology |
| title | Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control |
| title_full | Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control |
| title_fullStr | Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control |
| title_full_unstemmed | Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control |
| title_short | Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control |
| title_sort | combining two genetic sexing strains allows sorting of non transgenic males for aedes genetic control |
| url | https://doi.org/10.1038/s42003-023-05030-7 |
| work_keys_str_mv | AT celialutrat combiningtwogeneticsexingstrainsallowssortingofnontransgenicmalesforaedesgeneticcontrol AT myriamburckbuchler combiningtwogeneticsexingstrainsallowssortingofnontransgenicmalesforaedesgeneticcontrol AT roenickprovetiolmo combiningtwogeneticsexingstrainsallowssortingofnontransgenicmalesforaedesgeneticcontrol AT remybeugnon combiningtwogeneticsexingstrainsallowssortingofnontransgenicmalesforaedesgeneticcontrol AT albinfontaine combiningtwogeneticsexingstrainsallowssortingofnontransgenicmalesforaedesgeneticcontrol AT omarsakbari combiningtwogeneticsexingstrainsallowssortingofnontransgenicmalesforaedesgeneticcontrol AT rafaelargilesherrero combiningtwogeneticsexingstrainsallowssortingofnontransgenicmalesforaedesgeneticcontrol AT thierrybaldet combiningtwogeneticsexingstrainsallowssortingofnontransgenicmalesforaedesgeneticcontrol AT jeremybouyer combiningtwogeneticsexingstrainsallowssortingofnontransgenicmalesforaedesgeneticcontrol AT ericmarois combiningtwogeneticsexingstrainsallowssortingofnontransgenicmalesforaedesgeneticcontrol |