Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons
Peripheral nerve fibroblasts play a critical role in nerve development and regeneration. Our previous study found that peripheral nerve fibroblasts have different sensory and motor phenotypes. Fibroblasts of different phenotypes can guide the migration of Schwann cells to the same sensory or motor p...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Neural Regeneration Research |
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| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=8;spage=1833;epage=1840;aulast=He |
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| author | Qian-Ru He Meng Cong Fan-Hui Yu Yu-Hua Ji Shu Yu Hai-Yan Shi Fei Ding |
| author_facet | Qian-Ru He Meng Cong Fan-Hui Yu Yu-Hua Ji Shu Yu Hai-Yan Shi Fei Ding |
| author_sort | Qian-Ru He |
| collection | DOAJ |
| description | Peripheral nerve fibroblasts play a critical role in nerve development and regeneration. Our previous study found that peripheral nerve fibroblasts have different sensory and motor phenotypes. Fibroblasts of different phenotypes can guide the migration of Schwann cells to the same sensory or motor phenotype. In this study, we analyzed the different effects of peripheral nerve-derived fibroblasts and cardiac fibroblasts on motoneurons. Compared with cardiac fibroblasts, peripheral nerve fibroblasts greatly promoted motoneuron neurite outgrowth. Transcriptome analysis results identified 491 genes that were differentially expressed in peripheral nerve fibroblasts and cardiac fibroblasts. Among these, 130 were significantly upregulated in peripheral nerve fibroblasts compared with cardiac fibroblasts. These genes may be involved in axon guidance and neuron projection. Three days after sciatic nerve transection in rats, peripheral nerve fibroblasts accumulated in the proximal and distal nerve stumps, and most expressed brain-derived neurotrophic factor. In vitro, brain-derived neurotrophic factor secreted from peripheral nerve fibroblasts increased the expression of β-actin and F-actin through the extracellular regulated protein kinase and serine/threonine kinase pathways, and enhanced motoneuron neurite outgrowth. These findings suggest that peripheral nerve fibroblasts and cardiac fibroblasts exhibit different patterns of gene expression. Peripheral nerve fibroblasts can promote motoneuron neurite outgrowth. |
| first_indexed | 2024-04-11T17:23:49Z |
| format | Article |
| id | doaj.art-bda3059432fb4b55b66808910b2e370f |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-04-11T17:23:49Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-bda3059432fb4b55b66808910b2e370f2022-12-22T04:12:26ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742022-01-011781833184010.4103/1673-5374.332159Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneuronsQian-Ru HeMeng CongFan-Hui YuYu-Hua JiShu YuHai-Yan ShiFei DingPeripheral nerve fibroblasts play a critical role in nerve development and regeneration. Our previous study found that peripheral nerve fibroblasts have different sensory and motor phenotypes. Fibroblasts of different phenotypes can guide the migration of Schwann cells to the same sensory or motor phenotype. In this study, we analyzed the different effects of peripheral nerve-derived fibroblasts and cardiac fibroblasts on motoneurons. Compared with cardiac fibroblasts, peripheral nerve fibroblasts greatly promoted motoneuron neurite outgrowth. Transcriptome analysis results identified 491 genes that were differentially expressed in peripheral nerve fibroblasts and cardiac fibroblasts. Among these, 130 were significantly upregulated in peripheral nerve fibroblasts compared with cardiac fibroblasts. These genes may be involved in axon guidance and neuron projection. Three days after sciatic nerve transection in rats, peripheral nerve fibroblasts accumulated in the proximal and distal nerve stumps, and most expressed brain-derived neurotrophic factor. In vitro, brain-derived neurotrophic factor secreted from peripheral nerve fibroblasts increased the expression of β-actin and F-actin through the extracellular regulated protein kinase and serine/threonine kinase pathways, and enhanced motoneuron neurite outgrowth. These findings suggest that peripheral nerve fibroblasts and cardiac fibroblasts exhibit different patterns of gene expression. Peripheral nerve fibroblasts can promote motoneuron neurite outgrowth.http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=8;spage=1833;epage=1840;aulast=Hebrain-derived neurotrophic factor; differential gene expression; f-actin; fibroblasts; motoneurons; mrna sequencing; neurite outgrowth; peripheral nervous system; β-actin |
| spellingShingle | Qian-Ru He Meng Cong Fan-Hui Yu Yu-Hua Ji Shu Yu Hai-Yan Shi Fei Ding Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons Neural Regeneration Research brain-derived neurotrophic factor; differential gene expression; f-actin; fibroblasts; motoneurons; mrna sequencing; neurite outgrowth; peripheral nervous system; β-actin |
| title | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
| title_full | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
| title_fullStr | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
| title_full_unstemmed | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
| title_short | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
| title_sort | peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons |
| topic | brain-derived neurotrophic factor; differential gene expression; f-actin; fibroblasts; motoneurons; mrna sequencing; neurite outgrowth; peripheral nervous system; β-actin |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=8;spage=1833;epage=1840;aulast=He |
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