Protective effect of GLP-1 analog liraglutide on podocytes in mice with diabetic nephropathy
Protection of podocytes is one of the important means to delay the progression of diabetic nephropathy (DN), and glucagon-like peptide-1 (GLP-1) has been shown to have a protective effect on the kidney in DN models, but whether it h as a protective effect on podocytes and the potential mechanisms of...
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| Format: | Article |
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Bioscientifica
2023-09-01
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| Series: | Endocrine Connections |
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| Online Access: | https://ec.bioscientifica.com/view/journals/ec/12/10/EC-23-0284.xml |
| _version_ | 1797693098814341120 |
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| author | Shaomin Shi Xinghua Chen Wen Yu Xiaolan Ke Tean Ma |
| author_facet | Shaomin Shi Xinghua Chen Wen Yu Xiaolan Ke Tean Ma |
| author_sort | Shaomin Shi |
| collection | DOAJ |
| description | Protection of podocytes is one of the important means to delay the progression of diabetic nephropathy (DN), and glucagon-like peptide-1 (GLP-1) has been shown to have a protective effect on the kidney in DN models, but whether it h as a protective effect on podocytes and the potential mechanisms of action remain largely unknown. In the present study, we established a type 2 diabetes mellitus (T2DM) mouse model by high-fat diet feeding combined with streptozotocin (STZ) induction and admini stered the intervention for 14 weeks. We found that liraglutide significantly ameliorate d podocyte injury in DN mice. Mechanistically, we detected glucagon-like peptide-1 receptor (GLP-1R) protein expression levels in kidney tissues by immunohistochemical stai ning, immunofluorescence staining, and western blotting and found that podocytes could express GLP-1R and liraglutide treatment could restore GLP-1R expression in the kidney tissues of DN mice. Furthermore, we found that NLRP3-induced inflammation and pyropt osis were positively correlated with podocyte injury in DN mice, and liraglutide inh ibited the expression of NLRP3-induced inflammation and pyroptosis-related proteins. Our results suggest that liraglutide protects DN mouse podocytes by regulating GLP-1R in renal tissues and by regulating NLRP3-induced inflammation and pyroptosis. |
| first_indexed | 2024-03-12T02:37:38Z |
| format | Article |
| id | doaj.art-bda45bc0f8254949a85565ac0c12433e |
| institution | Directory Open Access Journal |
| issn | 2049-3614 |
| language | English |
| last_indexed | 2024-03-12T02:37:38Z |
| publishDate | 2023-09-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Connections |
| spelling | doaj.art-bda45bc0f8254949a85565ac0c12433e2023-09-04T11:58:16ZengBioscientificaEndocrine Connections2049-36142023-09-011210111https://doi.org/10.1530/EC-23-0284Protective effect of GLP-1 analog liraglutide on podocytes in mice with diabetic nephropathyShaomin Shi0Xinghua Chen1Wen Yu2Xiaolan Ke3Tean Ma4Division of Nephrology, The First Affiliated Hospital of Yangtze University, Jingzhou, ChinaDivision of Nephrology, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Immunology, School of Medicine, Yangtze University, Jingzhou, ChinaDivision of Nephrology, The First Affiliated Hospital of Yangtze University, Jingzhou, ChinaDivision of Nephrology, The First Affiliated Hospital of Yangtze University, Jingzhou, ChinaProtection of podocytes is one of the important means to delay the progression of diabetic nephropathy (DN), and glucagon-like peptide-1 (GLP-1) has been shown to have a protective effect on the kidney in DN models, but whether it h as a protective effect on podocytes and the potential mechanisms of action remain largely unknown. In the present study, we established a type 2 diabetes mellitus (T2DM) mouse model by high-fat diet feeding combined with streptozotocin (STZ) induction and admini stered the intervention for 14 weeks. We found that liraglutide significantly ameliorate d podocyte injury in DN mice. Mechanistically, we detected glucagon-like peptide-1 receptor (GLP-1R) protein expression levels in kidney tissues by immunohistochemical stai ning, immunofluorescence staining, and western blotting and found that podocytes could express GLP-1R and liraglutide treatment could restore GLP-1R expression in the kidney tissues of DN mice. Furthermore, we found that NLRP3-induced inflammation and pyropt osis were positively correlated with podocyte injury in DN mice, and liraglutide inh ibited the expression of NLRP3-induced inflammation and pyroptosis-related proteins. Our results suggest that liraglutide protects DN mouse podocytes by regulating GLP-1R in renal tissues and by regulating NLRP3-induced inflammation and pyroptosis.https://ec.bioscientifica.com/view/journals/ec/12/10/EC-23-0284.xmlliraglutidediabetic nephropathypodocytesglucagon-like peptide-1 receptorpyroptosis |
| spellingShingle | Shaomin Shi Xinghua Chen Wen Yu Xiaolan Ke Tean Ma Protective effect of GLP-1 analog liraglutide on podocytes in mice with diabetic nephropathy Endocrine Connections liraglutide diabetic nephropathy podocytes glucagon-like peptide-1 receptor pyroptosis |
| title | Protective effect of GLP-1 analog liraglutide on podocytes in mice with diabetic nephropathy |
| title_full | Protective effect of GLP-1 analog liraglutide on podocytes in mice with diabetic nephropathy |
| title_fullStr | Protective effect of GLP-1 analog liraglutide on podocytes in mice with diabetic nephropathy |
| title_full_unstemmed | Protective effect of GLP-1 analog liraglutide on podocytes in mice with diabetic nephropathy |
| title_short | Protective effect of GLP-1 analog liraglutide on podocytes in mice with diabetic nephropathy |
| title_sort | protective effect of glp 1 analog liraglutide on podocytes in mice with diabetic nephropathy |
| topic | liraglutide diabetic nephropathy podocytes glucagon-like peptide-1 receptor pyroptosis |
| url | https://ec.bioscientifica.com/view/journals/ec/12/10/EC-23-0284.xml |
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